##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940171_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 183524 Sequences flagged as poor quality 0 Sequence length 125 %GC 30 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.579384712626144 33.0 31.0 34.0 31.0 34.0 2 32.58182036136963 34.0 31.0 34.0 31.0 34.0 3 32.58596695800004 34.0 31.0 34.0 30.0 34.0 4 36.14429175475687 37.0 35.0 37.0 35.0 37.0 5 36.18785553933001 37.0 37.0 37.0 35.0 37.0 6 36.322115908546024 37.0 37.0 37.0 35.0 37.0 7 36.280290316252916 37.0 37.0 37.0 35.0 37.0 8 36.29633726379111 37.0 37.0 37.0 35.0 37.0 9 38.02767485451494 39.0 39.0 39.0 35.0 39.0 10-11 38.05107506375188 39.0 39.0 39.0 35.0 39.0 12-13 38.0456534295242 39.0 39.0 39.0 35.0 39.0 14-15 39.13928151086506 41.0 38.5 41.0 36.0 41.0 16-17 39.210345785837276 40.5 38.5 41.0 36.0 41.0 18-19 39.52396961705281 41.0 39.5 41.0 36.0 41.0 20-21 39.536136963013014 41.0 39.5 41.0 36.0 41.0 22-23 39.33438133432139 41.0 39.0 41.0 35.0 41.0 24-25 39.24282382685643 41.0 39.0 41.0 35.0 41.0 26-27 38.95891817963863 41.0 38.0 41.0 35.0 41.0 28-29 38.71956256402433 41.0 38.0 41.0 35.0 41.0 30-31 37.95495684488132 39.5 36.0 41.0 35.0 41.0 32-33 37.068451537673546 38.0 35.0 41.0 33.5 41.0 34-35 36.6443789368148 38.0 35.0 41.0 33.0 41.0 36-37 36.36941217497439 38.0 35.0 41.0 33.0 41.0 38-39 36.11433109566051 38.0 35.0 41.0 33.0 41.0 40-41 35.88303982040496 38.0 35.0 41.0 33.0 41.0 42-43 35.48491477953837 38.0 35.0 41.0 31.0 41.0 44-45 35.01281848695538 38.0 35.0 41.0 26.0 41.0 46-47 33.89743303328175 38.0 35.0 41.0 16.0 41.0 48-49 32.9817217366666 38.0 33.0 41.0 3.5 41.0 50-51 32.88852684117609 38.0 33.5 41.0 2.0 41.0 52-53 33.00881901004773 38.0 35.0 41.0 2.0 41.0 54-55 32.99144798500468 38.0 35.0 41.0 2.0 41.0 56-57 32.90086037793422 37.5 35.0 41.0 2.0 41.0 58-59 32.75684651598701 37.0 35.0 41.0 2.0 41.0 60-61 32.58784954556352 37.0 35.0 41.0 2.0 41.0 62-63 32.36918332207232 36.0 35.0 41.0 2.0 41.0 64-65 32.12490736906345 36.0 34.0 40.5 2.0 41.0 66-67 31.82239380135568 35.0 34.0 40.0 2.0 41.0 68-69 31.47591323205684 35.0 33.5 39.5 2.0 41.0 70-71 31.157578845273644 35.0 33.0 39.0 2.0 41.0 72-73 30.860516880625966 35.0 33.0 39.0 2.0 41.0 74-75 30.552284714805694 35.0 33.0 38.5 2.0 41.0 76-77 30.23420370087836 35.0 33.0 37.0 2.0 40.0 78-79 29.891662670822345 35.0 33.0 37.0 2.0 39.0 80-81 29.571355789978423 35.0 33.0 36.5 2.0 39.0 82-83 29.254010374664894 35.0 32.5 36.0 2.0 38.5 84-85 28.92570454000567 35.0 32.0 36.0 2.0 37.0 86-87 28.670451821015234 35.0 31.5 35.0 2.0 37.0 88-89 28.418029794468296 35.0 31.0 35.0 2.0 36.5 90-91 28.18516651773065 35.0 31.0 35.0 2.0 36.0 92-93 27.967538305616703 35.0 31.0 35.0 2.0 36.0 94-95 27.754549813648353 35.0 30.0 35.0 2.0 36.0 96-97 27.552873193696737 35.0 29.0 35.0 2.0 35.5 98-99 27.402402410583903 35.0 29.0 35.0 2.0 35.0 100-101 27.26524051350232 35.0 29.0 35.0 2.0 35.0 102-103 26.49552647065234 34.0 25.5 35.0 2.0 35.0 104-105 26.879274645278002 35.0 27.0 35.0 2.0 35.0 106-107 26.76108846799329 35.0 25.0 35.0 2.0 35.0 108-109 26.61124158148253 35.0 24.5 35.0 2.0 35.0 110-111 26.429317146531247 35.0 21.5 35.0 2.0 35.0 112-113 26.226477190994096 35.0 17.0 35.0 2.0 35.0 114-115 25.99168501122469 35.0 2.0 35.0 2.0 35.0 116-117 25.7715802837776 35.0 2.0 35.0 2.0 35.0 118-119 25.539730498463417 35.0 2.0 35.0 2.0 35.0 120-121 25.27747324600597 35.0 2.0 35.0 2.0 35.0 122-123 25.028464941914955 35.0 2.0 35.0 2.0 35.0 124-125 23.85969409995423 33.0 2.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.241413406007716 1101 2 0.14818922629216047 1101 3 0.006092074437844985 1101 4 -0.22736658754514139 1101 5 -0.15950464653431595 1101 6 0.06766430305211912 1101 7 0.053870956849365825 1101 8 0.10092129601311939 1101 9 0.1367465485520043 1101 10-11 0.07214449927108291 1101 12-13 0.19384434024271968 1101 14-15 -0.08505038095744766 1101 16-17 0.20145701793344983 1101 18-19 0.10160749844781947 1101 20-21 0.09784411141323801 1101 22-23 0.20074253671377562 1101 24-25 0.20940440181534825 1101 26-27 0.3331910148052941 1101 28-29 0.4369666250526265 1101 30-31 0.5010811678331919 1101 32-33 0.47279562129825337 1101 34-35 0.6224039571572817 1101 36-37 0.30969257119047455 1101 38-39 0.35789424154117455 1101 40-41 0.41093927246765816 1101 42-43 0.44737408541199386 1101 44-45 -0.04809233938336632 1101 46-47 -0.47905721284681846 1101 48-49 -0.4347664214387663 1101 50-51 -0.6504583899500318 1101 52-53 -0.6973647410491992 1101 54-55 -0.7316549879951069 1101 56-57 -0.6200546480430447 1101 58-59 -0.757863982353463 1101 60-61 -0.7701433548880559 1101 62-63 -0.9594769991007581 1101 64-65 -0.9241542934534799 1101 66-67 -0.7750777263175124 1101 68-69 -0.6852468119981232 1101 70-71 -0.7183055794442765 1101 72-73 -0.7678440215345255 1101 74-75 -0.8081562292175235 1101 76-77 -0.7414510808404664 1101 78-79 -0.6638152517502967 1101 80-81 -0.6183970505409029 1101 82-83 -0.7407482792982698 1101 84-85 -0.632794943402974 1101 86-87 -0.5921878753247292 1101 88-89 -0.7158179212438576 1101 90-91 -0.9602876685963508 1101 92-93 -0.8536836292834167 1101 94-95 -1.0420073991364909 1101 96-97 -1.15375729270907 1101 98-99 -0.9643861195834269 1101 100-101 -1.0677898698090047 1101 102-103 -1.1209222890336648 1101 104-105 -0.9956623250451138 1101 106-107 -1.3844186630606643 1101 108-109 -1.2067926284036936 1101 110-111 -1.0368659581176765 1101 112-113 -0.9096717977905158 1101 114-115 -0.9447895874102485 1101 116-117 -0.9546686794623582 1101 118-119 -1.0182852054329814 1101 120-121 -0.9027730787817951 1101 122-123 -0.7658714284311685 1101 124-125 -0.6865768360322377 1102 1 -0.06823151072914868 1102 2 0.18562524002896197 1102 3 0.0918174680748578 1102 4 0.0636494171201889 1102 5 -0.2665155970948092 1102 6 -0.19109938514849034 1102 7 0.11730645221643243 1102 8 0.09424728577531738 1102 9 0.03956842375449554 1102 10-11 0.14923579713929058 1102 12-13 0.15735597116348288 1102 14-15 0.32954010695806346 1102 16-17 -0.10817169974944107 1102 18-19 0.025650134357881882 1102 20-21 0.1643979246705456 1102 22-23 0.1711792632089555 1102 24-25 0.043023918435579844 1102 26-27 0.16854383020087482 1102 28-29 0.1731974291736762 1102 30-31 0.43520771484284637 1102 32-33 0.1779080427328381 1102 34-35 0.31612844364727266 1102 36-37 0.5380263364985751 1102 38-39 0.7776036305128571 1102 40-41 0.2996598711846943 1102 42-43 0.34109209228039106 1102 44-45 0.49223520548802213 1102 46-47 0.6008770189940336 1102 48-49 0.9365806918898159 1102 50-51 1.2092092054624004 1102 52-53 0.8298577691562059 1102 54-55 0.8317157111560647 1102 56-57 1.0121215328679867 1102 58-59 0.8163762820150993 1102 60-61 0.3911020283815674 1102 62-63 0.3869640687408733 1102 64-65 0.3960524064752491 1102 66-67 0.49562466466285215 1102 68-69 0.4826482829283343 1102 70-71 0.12652368252682322 1102 72-73 -0.1456545573992294 1102 74-75 0.23464167138508074 1102 76-77 -0.03319604293468004 1102 78-79 -0.11656261130449508 1102 80-81 -0.263832157130679 1102 82-83 0.06522755171319972 1102 84-85 0.013579708317365657 1102 86-87 0.03886247133502607 1102 88-89 0.01948143727360474 1102 90-91 0.12620718010448684 1102 92-93 0.1968574191193575 1102 94-95 -0.2835028957994865 1102 96-97 -0.5987777035170794 1102 98-99 -0.4095442223502239 1102 100-101 -0.4823155615086101 1102 102-103 -0.7199471059843532 1102 104-105 -0.7499307757675879 1102 106-107 -0.6303365014588991 1102 108-109 -0.7836247414082926 1102 110-111 -0.7215918701244135 1102 112-113 -0.7079368791097664 1102 114-115 -0.8058260028835846 1102 116-117 -0.552227482028286 1102 118-119 -0.6156658184051835 1102 120-121 -0.8866469205494418 1102 122-123 -0.6818388359345668 1102 124-125 -1.0278342066017956 1103 1 0.23536344503972373 1103 2 -0.04150632405912802 1103 3 -0.11581789173441592 1103 4 -0.1259484246515683 1103 5 -0.020875971020394957 1103 6 0.05543427442864868 1103 7 -0.0478074194191791 1103 8 0.06410110345522213 1103 9 0.11131069114893677 1103 10-11 0.10236177743917807 1103 12-13 0.2355760602531376 1103 14-15 0.30764412854033907 1103 16-17 0.30895766846688133 1103 18-19 0.05366318411006432 1103 20-21 0.05207908409082762 1103 22-23 0.050241625966968684 1103 24-25 0.14734351188560169 1103 26-27 -0.04125603177289605 1103 28-29 0.014184944074230543 1103 30-31 -0.3804566932128708 1103 32-33 -0.49936154758022866 1103 34-35 -0.6659124623066717 1103 36-37 -0.2517503119737228 1103 38-39 -0.54400531609609 1103 40-41 -0.9097086588360099 1103 42-43 -0.6554101977001636 1103 44-45 -0.8092815145069636 1103 46-47 -0.972096505066439 1103 48-49 -0.8449928070749877 1103 50-51 -0.5457550331974943 1103 52-53 -0.5868716367036342 1103 54-55 -0.974043746777312 1103 56-57 -1.0234829592582386 1103 58-59 -1.0062051220880477 1103 60-61 -0.755213612499297 1103 62-63 -0.8436495205683627 1103 64-65 -0.8077088081555388 1103 66-67 -0.9371581322503779 1103 68-69 -0.6421164450972618 1103 70-71 -0.5704068024471383 1103 72-73 -0.6345497202074348 1103 74-75 -0.5038692138649346 1103 76-77 -0.5617737454254232 1103 78-79 -0.9406497560438183 1103 80-81 -0.9408664754693419 1103 82-83 -0.7406181726107839 1103 84-85 -0.5021678291693021 1103 86-87 -0.7812328922385987 1103 88-89 -0.6231169064116955 1103 90-91 -0.5804086678157105 1103 92-93 -0.6055376495800573 1103 94-95 -0.68190461485338 1103 96-97 -0.5427437616135684 1103 98-99 -0.3393861195834269 1103 100-101 -0.3673930444121787 1103 102-103 -0.13081039728242416 1103 104-105 -0.3497802017039717 1103 106-107 -0.5097439297793755 1103 108-109 -0.24758107492984394 1103 110-111 -0.182097548021396 1103 112-113 -0.15732987741580473 1103 114-115 -0.26455929857340266 1103 116-117 -0.15350422460937807 1103 118-119 -0.15060370660394184 1103 120-121 -0.05460758307532032 1103 122-123 0.3146320844493964 1103 124-125 0.18855327065524818 1104 1 0.13959428235606453 1104 2 0.05474965044963653 1104 3 0.263511783134291 1104 4 0.2512274766879017 1104 5 -0.041307315801184075 1104 6 0.21773438654288668 1104 7 0.13492403747584802 1104 8 0.16320418954715876 1104 9 0.16869547901699633 1104 10-11 0.2867675719750835 1104 12-13 0.2228451934330451 1104 14-15 0.15773559015597272 1104 16-17 0.49641710081479573 1104 18-19 0.4031645707861671 1104 20-21 0.34978664295081074 1104 22-23 0.4467203537654001 1104 24-25 0.38088042107307274 1104 26-27 0.3410830252873467 1104 28-29 0.528730291333332 1104 30-31 0.4510400318712158 1104 32-33 0.5245659912172016 1104 34-35 0.6826300525927209 1104 36-37 0.9502175879495667 1104 38-39 0.8676678413949119 1104 40-41 0.8319049010860979 1104 42-43 1.136249093029761 1104 44-45 0.7142255958960604 1104 46-47 0.5738482049117195 1104 48-49 0.4228422079719252 1104 50-51 0.26688879756900974 1104 52-53 0.15793570382861333 1104 54-55 0.2269652813692673 1104 56-57 -0.022981395559646955 1104 58-59 -0.19236638956901686 1104 60-61 -0.034754239158878875 1104 62-63 -0.07461960376893018 1104 64-65 -0.08325905303664172 1104 66-67 -0.023036163760377093 1104 68-69 -0.020878172836685138 1104 70-71 0.04668619336332824 1104 72-73 -0.12437092750075962 1104 74-75 -0.09741821958866126 1104 76-77 -0.11042542418863022 1104 78-79 -0.20868392138281422 1104 80-81 -0.23296832240390586 1104 82-83 0.026825917788695364 1104 84-85 0.08233090216957706 1104 86-87 0.167578106758274 1104 88-89 0.138278915857736 1104 90-91 0.07072427606816234 1104 92-93 -0.010365937772661482 1104 94-95 0.0077823503912064496 1104 96-97 0.2769514646801703 1104 98-99 0.29165477260988126 1104 100-101 0.32858559487500827 1104 102-103 0.3268959814276684 1104 104-105 0.18721749700376478 1104 106-107 0.1030120152256444 1104 108-109 0.3173861759110146 1104 110-111 0.6009318967180839 1104 112-113 0.7751581735666662 1104 114-115 1.1072321689429714 1104 116-117 1.0178387859529607 1104 118-119 0.8782730857486882 1104 120-121 0.6085850173849394 1104 122-123 0.6554446176411091 1104 124-125 0.6860159484153243 1105 1 -0.06095281731427349 1105 2 0.01900580532092988 1105 3 0.11026776265312321 1105 4 0.02343761907410169 1105 5 -0.03939948105334423 1105 6 -0.019468391040057043 1105 7 -0.24758280127002763 1105 8 -0.007027977107817662 1105 9 0.07372458752509914 1105 10-11 0.12375291250956622 1105 12-13 0.14023314321210023 1105 14-15 -0.11493748272051363 1105 16-17 0.09975581162351688 1105 18-19 0.22862574775187028 1105 20-21 0.3385570715122199 1105 22-23 0.03614309347220512 1105 24-25 0.27712787846243003 1105 26-27 0.19471282117708455 1105 28-29 0.12547574970465547 1105 30-31 0.2071668467691623 1105 32-33 0.22342970728649902 1105 34-35 0.15081410253309713 1105 36-37 0.20325118548060317 1105 38-39 0.02683176087306549 1105 40-41 0.145435096779444 1105 42-43 0.14268803530373475 1105 44-45 0.44425996497790976 1105 46-47 0.3147333841219364 1105 48-49 -0.2729353048288061 1105 50-51 -0.5096139729998299 1105 52-53 -0.6486715768054623 1105 54-55 -0.8244480594457713 1105 56-57 -0.9985653192462607 1105 58-59 -0.8157513934267655 1105 60-61 -0.6505640168119626 1105 62-63 -0.6663359536321565 1105 64-65 -0.8051781103418207 1105 66-67 -0.8431105132027561 1105 68-69 -0.8049421414135232 1105 70-71 -0.6583148587514245 1105 72-73 -0.6402999448255855 1105 74-75 -0.5945775097619084 1105 76-77 -0.7221810530025472 1105 78-79 -0.5862247785056311 1105 80-81 -0.6851461999377477 1105 82-83 -0.6767442582651704 1105 84-85 -0.47464461862722374 1105 86-87 -0.2933323232059557 1105 88-89 -0.4683774634647051 1105 90-91 -0.5594293326854327 1105 92-93 -0.6656230044767355 1105 94-95 -0.5994772413882679 1105 96-97 -0.6042367235782287 1105 98-99 -0.5667446101494669 1105 100-101 -0.6012804358467392 1105 102-103 -0.22202034037916007 1105 104-105 -0.7595735530067564 1105 106-107 -0.7083438099830275 1105 108-109 -0.5949305807699155 1105 110-111 -0.7137836815943217 1105 112-113 -0.3458069663645915 1105 114-115 -0.2559788852760079 1105 116-117 0.026115421991399046 1105 118-119 0.0981159699459262 1105 120-121 0.013024941632675535 1105 122-123 0.1037156424008785 1105 124-125 0.1047856755668981 1106 1 0.09382382523923383 1106 2 0.09076357994990758 1106 3 0.23806252736339673 1106 4 0.11898538549963433 1106 5 0.18012471911072936 1106 6 0.1092958717685093 1106 7 0.2912194956946976 1106 8 0.09029471660535648 1106 9 0.19371862138295626 1106 10-11 0.15516465089422837 1106 12-13 0.15340340199351488 1106 14-15 0.16550848640469695 1106 16-17 0.18827098799759057 1106 18-19 -0.10478464825081346 1106 20-21 0.09918053336619437 1106 22-23 0.007147642655596087 1106 24-25 0.029189926340713157 1106 26-27 0.20016438356055488 1106 28-29 0.28979821968750485 1106 30-31 0.6921247108902762 1106 32-33 0.9150621929304634 1106 34-35 1.1560493922638742 1106 36-37 0.8562081546803952 1106 38-39 1.0009237886156228 1106 40-41 1.0170511755325222 1106 42-43 0.8529497997902666 1106 44-45 0.960614652128335 1106 46-47 0.869651722551346 1106 48-49 1.2389522333917995 1106 50-51 0.8811459643556816 1106 52-53 0.9108854371403936 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-0.5618978117624991 2216 26-27 -0.2768627038202496 2216 28-29 -0.5875772297218447 2216 30-31 -0.8450450709368411 2216 32-33 -0.9298162153230294 2216 34-35 -0.8225576062399611 2216 36-37 -0.8803486439618808 2216 38-39 -1.075246908778361 2216 40-41 -1.0510524845018736 2216 42-43 -1.0770539964626096 2216 44-45 -0.9676417512570268 2216 46-47 -1.2823937878213556 2216 48-49 -1.1789314672848334 2216 50-51 -0.4352828782338207 2216 52-53 -0.7348404287402204 2216 54-55 -0.9883698161881256 2216 56-57 -1.2909701801352895 2216 58-59 -1.260341522410883 2216 60-61 -1.1314242662523206 2216 62-63 -1.3105583179856382 2216 64-65 -1.2490593596755808 2216 66-67 -1.272206558400498 2216 68-69 -1.4156233054685323 2216 70-71 -1.5054686803056256 2216 72-73 -1.3423786144770098 2216 74-75 -1.4021406693397793 2216 76-77 -1.3316150152666957 2216 78-79 -1.3745682386911113 2216 80-81 -1.2670031448263543 2216 82-83 -1.436508675704676 2216 84-85 -1.5335775682061588 2216 86-87 -1.4990833092415592 2216 88-89 -1.5330240896239644 2216 90-91 -1.832022872819742 2216 92-93 -1.6843310313395428 2216 94-95 -1.6277951179472723 2216 96-97 -1.7602915151782739 2216 98-99 -1.7489906393574408 2216 100-101 -2.167366140995444 2216 102-103 -2.141437246892327 2216 104-105 -1.812451710985652 2216 106-107 -1.6368962031180843 2216 108-109 -1.9316004453898934 2216 110-111 -1.8182288366950594 2216 112-113 -2.410053848360114 2216 114-115 -2.6217021557162568 2216 116-117 -2.9499798770172347 2216 118-119 -2.9680976533351746 2216 120-121 -2.902367873632219 2216 122-123 -2.9351661399251014 2216 124-125 -2.852676215489492 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 14.0 5 68.0 6 138.0 7 258.0 8 418.0 9 716.0 10 1374.0 11 1812.0 12 2062.0 13 3124.0 14 5679.0 15 5948.0 16 2027.0 17 1432.0 18 1256.0 19 1339.0 20 1488.0 21 1824.0 22 1841.0 23 1397.0 24 1518.0 25 1741.0 26 1957.0 27 2139.0 28 2347.0 29 2603.0 30 3041.0 31 3572.0 32 4169.0 33 4933.0 34 7254.0 35 26009.0 36 10570.0 37 39601.0 38 37700.0 39 149.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.5622053252664 20.552148911236475 17.329735916932385 14.555909846564742 2 15.536265999705753 25.444776835096093 42.66588200676769 16.353075158430464 3 14.009917175239755 36.817785527462945 35.28552746294682 13.88676983435048 4 7.828861256544503 23.63983420593368 41.03457678883072 27.496727748691104 5 5.990115464883745 40.47046899776045 36.47687705360208 17.062538483753727 6 17.990559970350024 46.88130198284225 17.426992380391773 17.70114566641595 7 16.04422309888625 43.120790741265445 25.249558640831715 15.585427519016587 8 23.79143872191103 33.36021446786251 21.606438394978316 21.241908415248144 9 26.655369324992918 14.167084414027592 23.2650770471437 35.91246921383579 10-11 19.947254854950852 30.643676031472722 28.221649484536083 21.187419629040345 12-13 26.345873019332622 31.336500948104877 29.677862295939494 12.639763736623003 14-15 21.994398552777838 27.62472483162965 36.332850199428954 14.048026416163554 16-17 11.824611494954338 30.59490856781674 37.29294261241037 20.28753732481855 18-19 11.62954164033042 21.703973322290274 51.39654759050587 15.269937446873433 20-21 13.091475774285652 20.762407096619516 54.230236917242436 11.9158802118524 22-23 12.695342298554957 20.895632178897582 54.73997951221639 11.669046010331074 24-25 12.00969908321868 21.28294895721233 55.158086883079726 11.549265076489258 26-27 11.605021686536912 21.636407227392603 55.05410736470434 11.704463721366142 28-29 12.097360828449762 19.744610363250484 55.93234653872665 12.225682269573108 30-31 12.04423713019928 21.201998790263254 55.66805259630213 11.085711483235338 32-33 12.060547939234105 21.544048734770385 54.917612955253816 11.477790370741701 34-35 11.939582833852795 20.327041694819208 55.724591878991305 12.008783592336696 36-37 11.690873375889192 20.99867319444104 54.13693614169303 13.173517287976743 38-39 11.484873912948714 21.111407772280465 54.767223905320286 12.636494409450535 40-41 11.29661515660077 20.80191146662017 55.0391229484972 12.86235042828186 42-43 11.691113968745233 20.959928946622785 53.45377171378131 13.89518537085068 44-45 11.997220973476644 20.957674336234962 52.70723500483605 14.337869685452343 46-47 12.34443112467306 20.846501743679163 51.451340453356586 15.357726678291195 48-49 12.588271833656634 22.141245831607854 48.870175017981296 16.400307316754212 50-51 13.401427080750757 22.076432461591693 47.48054042714342 17.041600030514136 52-53 12.345251847169852 22.604945402236222 47.97002026982847 17.07978248076546 54-55 12.845158987688023 22.465882558079343 47.723551319879796 16.965407134352837 56-57 13.508624236133246 22.528994924355736 46.44500568047144 17.517375159039577 58-59 12.70188535309503 22.07743025283348 48.01138840453357 17.209295989537924 60-61 14.205226564373053 23.508914365423596 45.954480068002006 16.331379002201345 62-63 14.150267410275202 23.901547247022794 46.90591463032539 15.042270712376615 64-65 14.682868352223192 22.591543156059284 47.54113993025283 15.184448561464691 66-67 14.496196682722696 23.579749787493736 46.55412916021883 15.369924369564744 68-69 14.70442396865788 23.239866392768207 47.021081826256115 15.034627812317803 70-71 14.646479782692209 22.15880142873879 47.92132673272722 15.27339205584178 72-73 13.643509272856564 22.904650459487957 46.57189018060751 16.87995008704797 74-75 13.555992676707135 23.311120071489288 46.74238791656677 16.390499335236807 76-77 13.343631826157083 22.670034110351892 47.85475310861913 16.131580954871897 78-79 12.946570875119534 22.813699607952113 47.912392690854304 16.327336826074045 80-81 13.352822262059796 23.800559057992732 46.574543790151644 16.272074889795828 82-83 13.95523709626885 22.540287975588825 46.75121579097931 16.753259137163017 84-85 13.23232928663281 22.211536365815913 46.80505002070574 17.751084326845536 86-87 13.580139277890996 23.563901088721543 46.27584215172354 16.580117481663926 88-89 13.974650987913991 22.554735775237305 46.75726631720049 16.71334691964821 90-91 13.569116589289218 22.543810893873015 46.797554436670374 17.089518080167394 92-93 14.156569881018225 23.947296004969605 45.216889036620884 16.679245077391283 94-95 13.943591364334834 22.798036159940715 45.498534203729335 17.759838271995118 96-97 14.378621969261452 23.09341717729565 45.075756543725184 17.452204309717708 98-99 14.072653263620785 23.437529799935156 44.95805531412878 17.53176162231528 100-101 13.882910030133447 23.073339254673954 44.9800290981217 18.0637216170709 102-103 14.713887549347348 22.831781735555428 44.3814178797464 18.07291283535082 104-105 14.067599893200233 23.616643326921714 44.38156941167496 17.934187368203094 106-107 14.365968483686057 22.68340925437545 44.299655630871165 18.650966631067327 108-109 14.695576831906534 23.261548354049623 43.06363106298805 18.979243751055794 110-111 14.850344102309817 23.60411070122765 42.791833087221626 18.753712109240904 112-113 14.766984075088207 23.52119007724059 43.55197733186208 18.159848515809117 114-115 14.754473529347662 23.97506593143131 42.59197707111876 18.678483468102264 116-117 14.685000326932718 24.11591944377847 42.55192781325603 18.64715241603278 118-119 14.81725673963683 23.46306311215246 42.59750166873272 19.122178479477995 120-121 14.509273991412567 24.106656350123146 42.324709574769514 19.059360083694777 122-123 14.50273947597135 24.568372470650417 42.23206671734221 18.69682133603603 124-125 14.772910017144525 24.58617371845367 41.69689899449685 18.944017269904954 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3010.0 1 2117.0 2 2240.0 3 2783.0 4 1362.5 5 761.0 6 2114.5 7 5307.5 8 8795.0 9 9173.5 10 5239.0 11 1621.0 12 906.5 13 749.0 14 706.0 15 682.0 16 669.5 17 705.5 18 741.0 19 715.0 20 697.5 21 726.5 22 751.5 23 792.0 24 797.0 25 804.0 26 882.5 27 1049.0 28 1229.5 29 1691.0 30 2547.5 31 3125.5 32 4389.5 33 5938.5 34 6659.0 35 7319.0 36 9145.5 37 10453.5 38 9771.5 39 9081.0 40 8759.5 41 8022.0 42 5990.0 43 5132.5 44 5588.5 45 5079.5 46 3868.5 47 2880.5 48 2434.0 49 1976.5 50 1589.0 51 1080.0 52 687.5 53 507.0 54 404.5 55 433.0 56 474.5 57 377.5 58 232.5 59 186.0 60 194.0 61 212.0 62 179.0 63 95.0 64 44.5 65 60.0 66 73.0 67 49.0 68 29.0 69 17.5 70 12.0 71 12.0 72 11.5 73 26.0 74 27.5 75 11.0 76 6.0 77 8.5 78 7.0 79 2.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.032148383862601076 2 0.0027244393103899216 3 0.002179551448311937 4 0.08936160938078944 5 0.0027244393103899216 6 0.027244393103899218 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.173317931169765E-4 26-27 0.0 28-29 2.7244393103899214E-4 30-31 0.007083542207013797 32-33 0.0 34-35 0.0 36-37 2.7244393103899214E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0035417711035068983 46-47 0.002179551448311937 48-49 0.0 50-51 0.0013622196551949608 52-53 0.0 54-55 0.001907107517272945 56-57 2.7244393103899214E-4 58-59 0.002179551448311937 60-61 0.0 62-63 0.0024519953793509294 64-65 0.002179551448311937 66-67 0.0 68-69 5.448878620779843E-4 70-71 0.002996883241428914 72-73 0.0024519953793509294 74-75 0.0 76-77 0.0010897757241559686 78-79 2.7244393103899214E-4 80-81 5.448878620779843E-4 82-83 8.173317931169765E-4 84-85 0.0 86-87 0.003269327172467906 88-89 0.003269327172467906 90-91 0.004359102896623874 92-93 0.0046315468276628665 94-95 0.003269327172467906 96-97 0.0046315468276628665 98-99 0.0046315468276628665 100-101 0.00381421503454589 102-103 0.001907107517272945 104-105 0.001634663586233953 106-107 0.0 108-109 0.007083542207013797 110-111 0.001634663586233953 112-113 0.0035417711035068983 114-115 0.0 116-117 0.0 118-119 8.173317931169765E-4 120-121 0.0 122-123 0.001907107517272945 124-125 0.0454981364835117 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 183524.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.41489941370066 #Duplication Level Percentage of deduplicated Percentage of total 1 70.83719233251944 13.752969638848326 2 9.46647582161601 3.6758135175780824 3 4.3641772613735235 2.5419018765937973 4 2.6577979849007884 2.064035221551405 5 1.8438999747410965 1.7899566269261786 6 1.3050433611181274 1.5202371351975763 7 1.0777132272459375 1.4646585732656219 8 0.7633802026325391 1.1856759878816938 9 0.7353147540063428 1.2848455787798871 >10 5.803934775897393 23.13212440879667 >50 0.740927843731582 9.78891044223099 >100 0.3676573770031714 12.555306118000917 >500 0.005613089725239258 0.9012445238769861 >1k 0.022452358900957033 8.60704866938384 >5k 0.005613089725239258 9.13722455918572 >10k+ 0.002806544862619629 6.598047121902313 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12109 6.598047121902313 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9602 5.2320132516728055 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7167 3.905211307512914 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3432 1.8700551426516423 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2947 1.60578452954382 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2361 1.286480242366121 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2303 1.2548767463655979 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1367 0.7448617074606046 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1282 0.6985462391839758 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1087 0.5922931060787691 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1017 0.5541509557333101 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 933 0.5083803753187595 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 721 0.3928641485582267 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 470 0.25609729517665264 TruSeq Adapter, Index 3 (95% over 21bp) GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 449 0.24465465007301496 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 442 0.24084043503846908 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 401 0.21850003269327173 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 366 0.19942895752054227 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 350 0.19071075172729454 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 347 0.18907608814106058 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 345 0.1879863124169046 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 343 0.1868965366927486 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 337 0.18362720952028072 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 325 0.1770885551753449 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 320 0.17436411586495498 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 312 0.17000501296833112 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 301 0.16401124648547327 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 288 0.15692770427845948 No Hit ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT 274 0.14929927420936773 RNA PCR Primer, Index 3 (95% over 23bp) GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG 272 0.14820949848521173 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 266 0.14494017131274384 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 258 0.14058106841611998 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 256 0.13949129269196398 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 253 0.13785662910573004 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 247 0.13458730193326213 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 247 0.13458730193326213 No Hit TTCTAGAATTGTGAGTAGAAGTAAAATAATAAATGTAATGGTAGCTGTTG 243 0.13240775048495018 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 239 0.13022819903663826 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 231 0.12586909614001437 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 222 0.12096510538131254 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 220 0.11987532965715655 No Hit GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT 217 0.11824066607092261 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 212 0.11551622676053268 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 212 0.11551622676053268 No Hit TACTAGAGTAGCTCCTCCGATTAGGTGTATTAATAAGTGTCCTGCAGTAA 211 0.11497133889845469 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 204 0.1111571238639088 No Hit GGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAAT 202 0.11006734813975283 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 201 0.10952246027767486 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 188 0.10243891807066106 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 187 0.10189403020858308 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 184 0.10025936662234913 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.448878620779844E-4 0.0 5 0.0 0.0 0.0 5.448878620779844E-4 0.0 6 0.0 0.0 0.0 5.448878620779844E-4 0.0 7 0.0 0.0 0.0 5.448878620779844E-4 0.0 8 0.0 0.0 0.0 5.448878620779844E-4 0.0 9 0.0 0.0 0.0 5.448878620779844E-4 0.0 10-11 0.0 0.0 0.0 8.173317931169765E-4 0.0 12-13 0.0 0.0 0.0 0.0019071075172729453 0.0 14-15 0.0 0.0 0.0 0.0027244393103899216 0.0 16-17 0.0 0.0 0.0 0.00626621041389682 0.0 18-19 0.0 0.0 0.0 0.007628430069091781 0.0 20-21 0.0 0.0 0.0 0.01117020117259868 0.0 22-23 0.0 0.0 0.0 0.020978182690002397 0.0 24-25 0.0 0.0 0.0 0.040594145724809835 0.0 26-27 0.0 0.0 0.0 0.06811098275974804 0.0 28-29 0.0 0.0 0.0 0.09971447876027113 0.0 30-31 0.0 0.0 0.0 0.19615963034807438 0.0 32-33 0.0 0.0 0.0 0.2882456790392537 0.0 34-35 0.0 0.0 0.0 0.3759726248338092 0.0 36-37 0.0 0.0 0.0 0.47650443538719733 0.0 38-39 0.0 0.0 0.0 0.6388810182864366 0.0 40-41 0.0 0.0 0.0 0.8502975087726946 0.0 42-43 0.0 0.0 0.0 1.0219371853272596 0.0 44-45 0.0 0.0 0.0 1.1734160109849392 0.0 46-47 0.0 0.0 0.0 1.3458730193326214 0.0 48-49 0.0 0.0 0.0 1.560831280922386 0.0 50-51 0.0 0.0 0.0 1.7864148558226716 0.0 52-53 0.0 0.0 0.0 1.9787602711362 0.0 54-55 0.0 0.0 0.0 2.1675639153462214 0.0 56-57 0.0 0.0 0.0 2.363723545694296 0.0 58-59 0.0 0.0 0.0 2.579226695146139 0.0 60-61 0.0 0.0 0.0 2.821701793770842 0.0 62-63 0.0 0.0 0.0 3.068808439223208 0.0 64-65 0.0 0.0 0.0 3.307741766744404 0.0 66-67 0.0 0.0 0.0 3.561387066541706 0.0 68-69 0.0 0.0 0.0 3.837372768684205 0.0 70-71 0.0 0.0 0.0 4.170299252413853 0.0 72-73 0.0 0.0 0.0 4.524748806695582 0.0 74-75 0.0 0.0 0.0 4.834517556286916 0.0 76-77 0.0 0.0 0.0 5.143741418016172 0.0 78-79 0.0 0.0 0.0 5.5379677862295935 0.0 80-81 0.0 0.0 0.0 5.970336304788474 0.0 82-83 0.0 0.0 0.0 6.400525271899044 0.0 84-85 0.0 0.0 0.0 6.818999149974935 0.0 86-87 0.0 0.0 0.0 7.263082757568492 0.0 88-89 0.0 0.0 0.0 7.692999280748022 0.0 90-91 0.0 0.0 0.0 8.184488132342363 0.0 92-93 0.0 0.0 0.0 8.73727686842048 0.0 94-95 0.0 0.0 0.0 9.260641659946383 0.0 96-97 0.0 0.0 0.0 9.814792615679693 0.0 98-99 0.0 0.0 0.0 10.34578583727469 0.0 100-101 0.0 0.0 0.0 10.943255378043197 0.0 102-103 0.0 0.0 0.0 11.639622065778862 0.0 104-105 0.0 0.0 0.0 12.346886510756086 0.0 106-107 0.0 0.0 0.0 13.034807436629542 0.0 108-109 0.0 0.0 0.0 13.676685338157407 0.0 110-111 0.0 0.0 0.0 14.388581329962292 0.0 112-113 0.0 0.0 0.0 15.114916850112246 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1855 0.0 96.72126 1 TGGGGAC 20 8.446279E-4 89.31339 6 GGGAGTG 30 4.239691E-5 79.389694 5 GTATATG 25 0.0020394537 71.52875 1 CACGCAG 25 0.0020438759 71.489716 3 GGAGTGG 35 9.0999114E-5 68.0483 6 GTGGGTA 35 9.136851E-5 67.992676 9 GTGGTAT 310 0.0 63.452915 1 GTATCAA 3900 0.0 62.35839 1 GTCTAGA 30 0.0042008003 59.607292 1 ATCACGC 30 0.0042008003 59.607292 1 ATGTATT 50 7.348197E-6 59.60729 1 TCACGCA 30 0.0042098938 59.57476 2 TTAGGCA 30 0.0042098938 59.57476 4 TCTTAGG 30 0.0042098938 59.57476 2 TACAGGG 40 1.7570918E-4 59.574757 2 TAGGCAT 30 0.004219002 59.54227 5 GAGTGGG 40 1.7689819E-4 59.493595 7 GGAAGCT 40 1.7689819E-4 59.493595 8 GGAATGA 45 3.1655817E-4 52.883198 8 >>END_MODULE