FastQCFastQC Report
Fri 27 May 2016
SRR940170_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940170_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1732049
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG19110.11033175158439512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30500.062.6561361
GTATCAA53900.059.422641
TCAACGC66950.046.2406354
ATCAACG67400.046.0202333
CAACGCA68800.044.9972425
AACGCAG69850.043.9621246
TATCAAC76350.041.4041562
GTACATG144850.041.1820221
TACATGG150300.039.2926332
ACATGGG152350.037.2800873
ACGCAGA84850.036.0407457
CGCAGAG85150.035.5674938
CATGGGG50000.033.5776334
CATGGGA102100.033.41184
GCAGAGT95600.030.5593229
GTATAGG6400.029.766551
ATGGGAG39000.029.1567945
ATGGGAT40250.027.2159145
CCGTTGC3007.2759576E-1225.7835229
CGATACC4850.025.78057118-119
Produced by FastQC (version 0.11.5)