FastQCFastQC Report
Fri 27 May 2016
SRR940170_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940170_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1732049
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98240.5671894963710611No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75700.4370546098869027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44870.259057336137719No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG18030.10409636216989243No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50400.084.5166241
GTATCAA105300.063.9883421
ATCAACG141250.047.7349783
TCAACGC141950.047.456254
CAACGCA145400.046.287415
TATCAAC145950.046.239922
AACGCAG148550.045.3019646
TGGTATC13800.040.1416442
ACGCAGA171900.039.0723847
CGCAGAG174000.038.498258
GTGGTAT14950.038.2557451
GTACATG137700.037.2075541
TACATGG143750.035.3868032
GCAGAGT190100.034.7683269
ACATGGG140150.034.7222373
CATGGGA92850.032.651424
GAGTACT112600.029.98329212-13
ATGGGAG37400.029.9123765
GTACTTT115200.028.94509514-15
CAGAGTA184600.028.6981610-11
Produced by FastQC (version 0.11.5)