FastQCFastQC Report
Fri 27 May 2016
SRR940169_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940169_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985703
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG21720.10938191663103698No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20780.10464807677683922No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38200.058.2717481
GGTATCA21000.058.10111
TCAACGC46550.047.1798444
ATCAACG46950.046.777883
AACGCAG49650.044.0986446
TATCAAC50850.043.892482
CAACGCA50250.043.70155
GTACATG103300.038.027121
ACGCAGA62350.034.922757
TACATGG112100.034.5640952
CGCAGAG63400.034.250548
ACATGGG110300.034.0489433
CATGGGG59750.029.7840064
TAAGGTG10000.027.9707375
CATGGGA57350.027.398094
CGATACC4050.027.199316118-119
GCAGAGT75300.027.1786169
ATGGGAG28400.024.9364515
ATGGGGA33600.024.0882725
GAGTACA80400.023.6888371
Produced by FastQC (version 0.11.5)