FastQCFastQC Report
Fri 27 May 2016
SRR940169_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940169_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985703
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49170.24762011237330056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37780.19026007413998972No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22300.11230279654107388No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20150.10147539687455778No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33500.078.0995941
GTATCAA69150.063.0881731
ATCAACG93450.046.4105573
TCAACGC94150.046.0643424
TATCAAC94800.045.9428142
CAACGCA96800.044.8173565
AACGCAG99200.043.7319646
ACGCAGA115550.037.5317277
CGCAGAG115800.037.296588
GTACATG99800.035.889961
CTATACG851.6985444E-435.043294
TACATGG109250.032.6128162
GCAGAGT132300.032.1504559
GTGGTAT18000.031.454311
ACATGGG107750.031.4047853
TAAGGTG9950.028.1105185
TGGTATC17850.027.7043782
GAGTACT73900.027.69204112-13
CATGGGG54900.027.4537664
AGAGTAC122000.027.30669410-11
Produced by FastQC (version 0.11.5)