FastQCFastQC Report
Fri 27 May 2016
SRR940168_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940168_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385157
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28300.7347653034996119No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6190.1607136829916112No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5540.14383744810557772No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG4910.12748048198526835No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4890.12696121321954423No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC4020.10437302191054557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5500.079.0198061
GATAGTC701.7462298E-1067.996377
GTATCAA13000.065.4890061
TAGAGAC300.004224433659.5354652
TCGACCG504.14002E-947.60363418-19
TCAACGC16550.045.3260964
ATCAACG17300.043.361093
GTACAAG3300.043.2985231
CAACGCA17450.042.9883655
AACGCAG17650.042.49026
TATCAAC20250.042.042332
TCACTAC1053.2410026E-739.6903153
GTACATG33100.039.3905371
AGTATCG650.001931640336.637215
GGATAGC650.001936574736.6181838
TAGTCAA1304.1874955E-836.6181839
GTTCGAC654.206413E-836.60867316-17
TACATGG36750.035.1542782
AGACCGT851.7050031E-435.0117656
CGCAGAG21150.034.8868268
Produced by FastQC (version 0.11.5)