FastQCFastQC Report
Fri 27 May 2016
SRR940168_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940168_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385157
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45261.1751052168336549No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39681.0302292311966288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24740.6423354632007207No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT19330.5018732620723497No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT9230.23964253538167551RNA PCR Primer, Index 31 (95% over 22bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7800.20251481863240184No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7390.19186980893505765No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7020.18226333676916168No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6900.17914772417481703No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT6320.1640889299688179RNA PCR Primer, Index 31 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5710.14825123261423262No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5700.1479915982313706No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5610.1456548887856121No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.13085572896247505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4990.12955755704816477No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG4580.11891254735082057No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC4210.10930607518492459No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15800.092.340431
GTGGTAT2950.074.6900251
GTATCAA33850.062.804741
ATCAACG48200.043.9716763
TCAACGC48500.043.6996884
TATCAAC48750.043.4755822
CAACGCA49200.042.9346285
AGGCTTA1700.042.0026636
AACGCAG50300.041.9956976
TGGTATC5400.041.894782
CGCAGAG56550.037.3494158
GATTCGT401.1975727E-437.18502470-71
ACGCAGA56950.037.0870867
GTACATG26000.036.8752861
GGGTATA650.00192930236.646251
GTGTAAG650.00192930236.646251
ATATAGG1801.4551915E-1136.382312
GTGGTAC1001.0542484E-535.7300951
GCAGAGT60400.034.476189
ACATGGG27600.034.081453
Produced by FastQC (version 0.11.5)