##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940168_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 385157 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.72538211690298 34.0 31.0 34.0 31.0 34.0 2 32.7719942776582 34.0 31.0 34.0 31.0 34.0 3 32.824422248589535 34.0 31.0 34.0 31.0 34.0 4 36.2644557933518 37.0 37.0 37.0 35.0 37.0 5 36.239676287851445 37.0 37.0 37.0 35.0 37.0 6 36.320105826974455 37.0 37.0 37.0 35.0 37.0 7 36.282069389885166 37.0 37.0 37.0 35.0 37.0 8 36.28328967148462 37.0 37.0 37.0 35.0 37.0 9 38.087335813707135 39.0 39.0 39.0 35.0 39.0 10-11 38.066570255765825 39.0 39.0 39.0 35.0 39.0 12-13 38.070441404414304 39.0 39.0 39.0 35.0 39.0 14-15 39.4581612173737 41.0 39.0 41.0 36.0 41.0 16-17 39.47104038093556 41.0 39.0 41.0 36.5 41.0 18-19 39.598340417024744 41.0 40.0 41.0 37.0 41.0 20-21 39.597910722121114 41.0 40.0 41.0 37.0 41.0 22-23 39.529406190202955 41.0 40.0 41.0 36.0 41.0 24-25 39.4748440246445 41.0 39.0 41.0 36.0 41.0 26-27 39.314317278408545 41.0 39.0 41.0 35.0 41.0 28-29 39.16303611254631 41.0 39.0 41.0 35.0 41.0 30-31 38.83339261651742 40.5 38.5 41.0 35.0 41.0 32-33 38.427347808815625 40.0 37.0 41.0 35.0 41.0 34-35 38.177258884039496 40.0 37.0 41.0 35.0 41.0 36-37 37.95080447713529 40.0 37.0 41.0 34.0 41.0 38-39 37.69466087855083 40.0 36.0 41.0 33.5 41.0 40-41 37.47947590203475 40.0 36.0 41.0 33.0 41.0 42-43 37.15024133015887 40.0 36.0 41.0 33.0 41.0 44-45 36.9092201362042 40.0 36.0 41.0 32.5 41.0 46-47 36.15951417214279 40.0 36.0 41.0 28.5 41.0 48-49 35.42672987898442 40.0 35.0 41.0 21.5 41.0 50-51 35.080154326677174 40.0 35.0 41.0 18.0 41.0 52-53 34.92129313500728 40.0 35.0 41.0 17.5 41.0 54-55 34.75077435954689 40.0 35.0 41.0 15.0 41.0 56-57 34.58287269866574 39.0 35.0 41.0 14.5 41.0 58-59 34.362549557712825 39.0 35.0 41.0 12.5 41.0 60-61 34.108738774058374 39.0 35.0 41.0 9.0 41.0 62-63 33.81780546634229 38.0 35.0 41.0 6.5 41.0 64-65 33.475964347006546 37.0 35.0 40.5 2.0 41.0 66-67 33.11390420010541 37.0 34.0 40.0 2.0 41.0 68-69 32.710653058363214 36.0 34.0 39.5 2.0 41.0 70-71 32.382640066258695 36.0 34.0 39.0 2.0 41.0 72-73 32.14779297792848 35.5 34.0 39.0 2.0 41.0 74-75 31.85273537804064 35.0 34.0 37.5 2.0 40.5 76-77 31.54649013259528 35.0 34.0 37.0 2.0 39.0 78-79 31.251162512949264 35.0 34.0 37.0 2.0 39.0 80-81 30.978530832881138 35.0 34.0 36.0 2.0 39.0 82-83 30.728023896748596 35.0 33.5 36.0 2.0 37.0 84-85 30.514518754689647 35.0 33.0 35.5 2.0 37.0 86-87 30.32994597008493 35.0 33.0 35.0 2.0 36.5 88-89 30.165442923275442 35.0 33.0 35.0 2.0 36.0 90-91 30.042667016307636 35.0 33.0 35.0 2.0 36.0 92-93 29.919531515719562 35.0 33.0 35.0 2.0 36.0 94-95 29.815338939705107 35.0 33.0 35.0 2.0 36.0 96-97 29.747443250414765 35.0 33.0 35.0 2.0 35.0 98-99 29.677361439620725 35.0 33.0 35.0 2.0 35.0 100-101 29.62889159485612 35.0 33.0 35.0 2.0 35.0 102-103 28.88761725737816 34.5 30.5 35.0 2.0 35.0 104-105 29.429648169447784 35.0 32.5 35.0 2.0 35.0 106-107 29.46016559480939 35.0 33.0 35.0 2.0 35.0 108-109 29.425371991162045 35.0 33.0 35.0 2.0 35.0 110-111 29.347035105164906 35.0 33.0 35.0 2.0 35.0 112-113 29.2340837632446 35.0 33.0 35.0 2.0 35.0 114-115 29.10475728079718 35.0 32.5 35.0 2.0 35.0 116-117 28.971161110923596 35.0 32.0 35.0 2.0 35.0 118-119 28.835818899825266 35.0 32.0 35.0 2.0 35.0 120-121 28.6595154703147 35.0 32.0 35.0 2.0 35.0 122-123 28.4434840857105 35.0 32.0 35.0 2.0 35.0 124-125 26.756608344129795 33.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15415307657356436 1101 2 0.07160042017950019 1101 3 0.13456911193927112 1101 4 -0.17277522405315437 1101 5 7.554775900402433E-4 1101 6 0.09346803019823113 1101 7 0.18933243843329706 1101 8 0.16736060998858449 1101 9 0.2500461897522257 1101 10-11 0.2200278395489832 1101 12-13 0.2665567088687055 1101 14-15 0.24877849029341803 1101 16-17 0.2710286723084181 1101 18-19 0.31075173911991527 1101 20-21 0.21768149543068205 1101 22-23 0.2011604854115916 1101 24-25 0.23342604157588198 1101 26-27 0.1824425178673934 1101 28-29 0.3444825812155372 1101 30-31 0.4980618808339443 1101 32-33 0.5775252626209948 1101 34-35 0.6739140571256996 1101 36-37 0.6308066808856481 1101 38-39 0.6269012558586695 1101 40-41 0.6543817989888296 1101 42-43 0.8279132693704767 1101 44-45 0.9794711429927645 1101 46-47 0.8189158423212959 1101 48-49 0.7688035039311458 1101 50-51 0.7028579306105343 1101 52-53 0.6056200411729975 1101 54-55 0.5455249187074926 1101 56-57 0.5004868247715706 1101 58-59 0.5417571871462172 1101 60-61 0.5397642517538799 1101 62-63 0.7623439275787831 1101 64-65 0.7787855417308194 1101 66-67 0.832881296508198 1101 68-69 0.7948482832056598 1101 70-71 0.8821479790130979 1101 72-73 0.7019147854945444 1101 74-75 0.7861483986836397 1101 76-77 0.8651672887824979 1101 78-79 0.7835328534120158 1101 80-81 0.7539086104912549 1101 82-83 0.6928310310449568 1101 84-85 0.7181666797492348 1101 86-87 0.7137628416838062 1101 88-89 0.6616232534675248 1101 90-91 0.666406267832329 1101 92-93 0.6734179880811944 1101 94-95 0.7854321504456934 1101 96-97 0.5351240498857344 1101 98-99 0.6927537768554473 1101 100-101 0.6782206684401046 1101 102-103 0.5170023736811586 1101 104-105 0.47420330343331685 1101 106-107 0.5064895149215083 1101 108-109 0.457840419565013 1101 110-111 0.4755835680626035 1101 112-113 0.6154793010503532 1101 114-115 0.8290765772895483 1101 116-117 0.689076222016272 1101 118-119 0.683921624806878 1101 120-121 0.6469618220898958 1101 122-123 0.7009562697958529 1101 124-125 0.4255730540559952 1102 1 0.08006054673058571 1102 2 0.12994648799337938 1102 3 0.12330740175390531 1102 4 0.06614577787362919 1102 5 -0.08299311054364011 1102 6 -0.014232454819371299 1102 7 0.12334568229305631 1102 8 0.06508612102882694 1102 9 0.033449285892018565 1102 10-11 0.08991466877406395 1102 12-13 0.1208797881768362 1102 14-15 0.1890094826970241 1102 16-17 0.07599179768214981 1102 18-19 0.1636906357602257 1102 20-21 0.2292532625336392 1102 22-23 0.1749163820297639 1102 24-25 0.10189546774911662 1102 26-27 0.30213002478830475 1102 28-29 0.3223190483384286 1102 30-31 0.3516098178481002 1102 32-33 0.34956698742333003 1102 34-35 0.2264797781187724 1102 36-37 0.5795476283309924 1102 38-39 0.6634381415584016 1102 40-41 0.4228467957775237 1102 42-43 0.4908758632180579 1102 44-45 0.4685582468396845 1102 46-47 0.5238191710037796 1102 48-49 0.5070490247160322 1102 50-51 0.1771232064504602 1102 52-53 0.2786318011889506 1102 54-55 0.2887258134431647 1102 56-57 0.1003694460813449 1102 58-59 0.04497402766622827 1102 60-61 0.1382405434731453 1102 62-63 0.19459095435441753 1102 64-65 0.3143424261017316 1102 66-67 0.39606093019809663 1102 68-69 0.3700149387988674 1102 70-71 0.471150924553811 1102 72-73 0.5980180344481099 1102 74-75 0.6059278153336898 1102 76-77 0.4027450798484331 1102 78-79 0.4985905461456106 1102 80-81 0.5635724202048955 1102 82-83 0.551284511433856 1102 84-85 0.4483341104847334 1102 86-87 0.527413097480764 1102 88-89 0.4027755578548309 1102 90-91 0.4349875359651243 1102 92-93 0.44354400407127414 1102 94-95 0.43479653376462934 1102 96-97 0.6040120528977155 1102 98-99 0.5466782115552462 1102 100-101 0.41280087352060946 1102 102-103 0.3161297849273659 1102 104-105 0.5079219932213697 1102 106-107 0.5168819583914903 1102 108-109 0.5262079698938926 1102 110-111 0.6726967166906022 1102 112-113 0.6371888220124156 1102 114-115 0.6540051534638849 1102 116-117 0.8231204715677976 1102 118-119 0.571592432948087 1102 120-121 0.5356901457893173 1102 122-123 0.7162321097178967 1102 124-125 0.6179080041146854 1103 1 0.14061075865814132 1103 2 0.056833297593009036 1103 3 0.047896814827254275 1103 4 -0.07363916836293072 1103 5 0.08821018723821084 1103 6 0.17078793820991933 1103 7 0.14406950760368176 1103 8 0.16982849999747174 1103 9 0.23517669543202402 1103 10-11 0.08254991345406637 1103 12-13 0.07626776465033203 1103 14-15 0.055486032530900786 1103 16-17 0.2612412033398499 1103 18-19 0.18480160146649638 1103 20-21 0.20238971770896086 1103 22-23 0.11602742043844927 1103 24-25 0.2187064487228909 1103 26-27 0.07890996182801047 1103 28-29 0.23122836660781587 1103 30-31 0.004650233124337433 1103 32-33 -0.031553647705024446 1103 34-35 -0.14264859254725337 1103 36-37 -0.1532273504035473 1103 38-39 0.16869634420913115 1103 40-41 0.255969292087876 1103 42-43 0.20870504160799186 1103 44-45 0.19732550457703724 1103 46-47 0.1839862411688813 1103 48-49 0.07393525269628043 1103 50-51 -0.13867793788566019 1103 52-53 -0.046184443440168366 1103 54-55 -0.06604564649416744 1103 56-57 -0.1531438984481639 1103 58-59 -0.1347554466225631 1103 60-61 0.014929965997808381 1103 62-63 -0.14843179423333197 1103 64-65 -0.07606230827997251 1103 66-67 -0.1017561795898203 1103 68-69 0.09902029696734616 1103 70-71 -0.05290812435310244 1103 72-73 -0.018751142633185225 1103 74-75 0.25951895145444226 1103 76-77 0.22628357941585264 1103 78-79 0.2870628501580619 1103 80-81 0.29606931217636756 1103 82-83 0.2977657142227237 1103 84-85 0.34952606668879227 1103 86-87 0.18011544649600708 1103 88-89 0.14932219282324155 1103 90-91 0.33218449409579875 1103 92-93 0.2316382554907861 1103 94-95 0.20778867463284456 1103 96-97 0.40272449300909585 1103 98-99 0.3496092096867933 1103 100-101 0.13875636509803613 1103 102-103 0.20274308801649 1103 104-105 0.15687834995761207 1103 106-107 0.05544514043108251 1103 108-109 0.19882896489923851 1103 110-111 0.08668066399588881 1103 112-113 0.1854723178356643 1103 114-115 0.3116203686267056 1103 116-117 0.24996729483548208 1103 118-119 0.40904072335064257 1103 120-121 0.25343208127318917 1103 122-123 0.4326399557632463 1103 124-125 0.38742678678533693 1104 1 0.0404619748044297 1104 2 -0.062168628690706385 1104 3 -0.059816945163547075 1104 4 -0.05696028273243314 1104 5 -0.10180188797311018 1104 6 0.014568485619498972 1104 7 -0.24690858451292996 1104 8 0.011751046142023824 1104 9 -0.08554235465135207 1104 10-11 0.1087452156218518 1104 12-13 0.01631038727122558 1104 14-15 0.04499389579351032 1104 16-17 0.03442309339792615 1104 18-19 0.004316724231145486 1104 20-21 -0.08606587713894953 1104 22-23 -0.10394986666408101 1104 24-25 -0.019371077008806026 1104 26-27 -0.10746158092398872 1104 28-29 3.3846665956360766E-4 1104 30-31 -0.37096071506725536 1104 32-33 -0.32395704601449893 1104 34-35 -0.35862776280773545 1104 36-37 -0.32046543331999544 1104 38-39 -0.14068063238795503 1104 40-41 -0.147860274929549 1104 42-43 -0.42170903751338784 1104 44-45 -0.3739182421885303 1104 46-47 -0.293056482078768 1104 48-49 -0.20166527343792495 1104 50-51 -0.04202952778848612 1104 52-53 -0.12272007968880416 1104 54-55 -0.060814851830258476 1104 56-57 -0.21558100672255875 1104 58-59 -0.02730044115996577 1104 60-61 -0.06399735273026863 1104 62-63 -0.0702340195222817 1104 64-65 -0.06556657772968322 1104 66-67 -0.04507456266153298 1104 68-69 0.058505447332478866 1104 70-71 0.004077169764542532 1104 72-73 0.05664525492877459 1104 74-75 -0.048558861782293405 1104 76-77 0.020180206981837756 1104 78-79 -0.18468618670008397 1104 80-81 -0.22538395388079024 1104 82-83 -0.130392169165237 1104 84-85 -0.06319279651631149 1104 86-87 -0.18552446782749143 1104 88-89 -0.22067881344436557 1104 90-91 -0.3097333800920019 1104 92-93 -0.461018866008839 1104 94-95 -0.4704303035742754 1104 96-97 -0.32211342602948534 1104 98-99 -0.3749627671978004 1104 100-101 -0.227361526340065 1104 102-103 -0.2132371603878589 1104 104-105 -0.3604492188998414 1104 106-107 -0.2278389576656359 1104 108-109 -0.28256945957319246 1104 110-111 -0.1822209949081426 1104 112-113 -0.0511928394295893 1104 114-115 -0.18638147140549677 1104 116-117 -0.3186637499577998 1104 118-119 -0.16722897074400578 1104 120-121 -0.1745371269379561 1104 122-123 -0.1457824322535437 1104 124-125 0.10018769760127455 1105 1 -0.04909819524135628 1105 2 -0.06395356437851518 1105 3 -0.07316989916398597 1105 4 0.002596617202165419 1105 5 -0.23636696318566663 1105 6 -0.032507489963670366 1105 7 -0.15387392133438027 1105 8 -0.032477207618605064 1105 9 -0.1386544104612497 1105 10-11 0.04316586092379282 1105 12-13 0.0725494869006198 1105 14-15 0.03991841072701163 1105 16-17 -0.07066329203917832 1105 18-19 0.08842670897062277 1105 20-21 0.1316485876528546 1105 22-23 0.043559398650941716 1105 24-25 0.16575127965132452 1105 26-27 0.15585049668664652 1105 28-29 0.18826766875243806 1105 30-31 0.19376023544505472 1105 32-33 0.20445204489459456 1105 34-35 0.2843782868979545 1105 36-37 0.2572950855376419 1105 38-39 0.5703505426719886 1105 40-41 0.5204686982555486 1105 42-43 0.5748914094018076 1105 44-45 0.5158790109180771 1105 46-47 0.745176567844922 1105 48-49 0.5325768782952309 1105 50-51 0.44743798910708676 1105 52-53 0.4036111280722636 1105 54-55 0.7129002669837803 1105 56-57 0.6012859542541094 1105 58-59 0.7804442721602811 1105 60-61 0.700118627213044 1105 62-63 0.5203889366582288 1105 64-65 0.6590287460113231 1105 66-67 1.0014972707808099 1105 68-69 0.8834386825624776 1105 70-71 0.7619458748005172 1105 72-73 0.6808345398644633 1105 74-75 0.5054047854817121 1105 76-77 0.672587826340898 1105 78-79 0.677492252366843 1105 80-81 0.6692844023433224 1105 82-83 0.5640363789053957 1105 84-85 0.6372037044647207 1105 86-87 0.6824924822488114 1105 88-89 0.5999419626620224 1105 90-91 0.4777843330102449 1105 92-93 0.4619155137600721 1105 94-95 0.5488156632885968 1105 96-97 0.5776917298458137 1105 98-99 0.3574399469944822 1105 100-101 0.4067470958530883 1105 102-103 0.4131935132550488 1105 104-105 0.38921695725014516 1105 106-107 0.5740672446452457 1105 108-109 0.42243926072329785 1105 110-111 0.5409572806532275 1105 112-113 0.4762692817825318 1105 114-115 0.4331955942147374 1105 116-117 0.25648067995281565 1105 118-119 0.38864224137720527 1105 120-121 0.40169734003391966 1105 122-123 0.13443203253826042 1105 124-125 0.3807632371696208 1106 1 -0.0076718867468628105 1106 2 -0.023890202224201573 1106 3 0.11264621858401824 1106 4 0.1273078573843307 1106 5 -0.038165524336740475 1106 6 0.02636651175238569 1106 7 0.054425860858209774 1106 8 -0.11708548430000576 1106 9 0.13955494865696494 1106 10-11 -0.0606424685594078 1106 12-13 0.011433058942067476 1106 14-15 -0.0017933574731046065 1106 16-17 -0.022574057005186887 1106 18-19 -0.1946998173312693 1106 20-21 0.058386096728156645 1106 22-23 -0.12739301373107992 1106 24-25 -0.2036583302754167 1106 26-27 -0.3362737855389426 1106 28-29 -0.1639599069851343 1106 30-31 -0.11334325607365514 1106 32-33 -0.16274978996779055 1106 34-35 -0.18515507723642344 1106 36-37 -0.17603604772087778 1106 38-39 -0.13647573942159852 1106 40-41 -0.1687943866893491 1106 42-43 -0.12057093631795368 1106 44-45 -0.33863884268895106 1106 46-47 -0.07438814735539268 1106 48-49 -0.1192893351560258 1106 50-51 0.13480185947868506 1106 52-53 0.1420821860882313 1106 54-55 0.21783228467238303 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114-115 0.13844719674742123 2216 116-117 0.07203994915870027 2216 118-119 0.3130291623581307 2216 120-121 0.3769707997402705 2216 122-123 0.3212594098734485 2216 124-125 0.003116852914626378 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 9.0 4 7.0 5 37.0 6 107.0 7 240.0 8 491.0 9 960.0 10 1922.0 11 2795.0 12 3214.0 13 5095.0 14 9781.0 15 9859.0 16 2623.0 17 1904.0 18 1724.0 19 1890.0 20 2096.0 21 2271.0 22 2335.0 23 1412.0 24 1494.0 25 1845.0 26 2261.0 27 2662.0 28 3120.0 29 3920.0 30 4782.0 31 5934.0 32 7501.0 33 10085.0 34 14538.0 35 25587.0 36 51804.0 37 125853.0 38 72793.0 39 206.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.5115617144163 19.76528577067708 14.893887769305882 20.82926474560073 2 15.811666225275609 22.576892918529595 39.67430532836898 21.937135527825813 3 16.499719591226324 29.596626786307745 32.62036806247923 21.283285559986705 4 11.139549688835764 19.29815899907755 38.31568553573517 31.246605776351515 5 10.862312048377339 39.472761782109835 34.47768208361137 15.187244085901455 6 26.592929431960133 40.18765857877064 17.654605374213435 15.564806615055796 7 24.39161173235849 36.21120737777062 22.38593612474913 17.011244765121756 8 26.183608242872385 32.929169144011404 21.223552992675714 19.663669620440498 9 27.73414477732457 14.161757413210715 20.68455201385409 37.41954579561062 10-11 23.34203973963864 27.72349976762723 27.729601175624484 21.204859317109644 12-13 26.248646655779336 26.378074395636062 28.19668343039332 19.176595518191284 14-15 23.186388927112837 25.85049732966037 28.74308398912651 22.220029754100278 16-17 19.26876572410731 29.398401171470333 29.358936745275305 21.973896359147048 18-19 19.30277782826224 25.149873947507125 35.59626334196185 19.951084882268788 20-21 20.74881152361245 24.367465734752322 35.95183262929143 18.931890112343797 22-23 20.357542508639334 24.789241789711728 35.92119577211371 18.93201992953523 24-25 19.73893627240981 24.761849125676676 36.52789136841012 18.971323233503394 26-27 19.33289541667424 25.14403217389272 36.14266390069504 19.380408508737997 28-29 19.818254988251482 23.88116472588958 36.622658410250416 19.67792187560852 30-31 19.496669998831578 24.164254092720736 36.707907616809685 19.631168291637994 32-33 18.908912469460507 25.028624690710544 36.17148331719273 19.890979522636222 34-35 19.928886142534083 23.990216976453755 36.44163289256069 19.639263988451464 36-37 19.861016632308115 24.150988955067323 35.36793074977801 20.62006366284655 38-39 19.466632394883636 24.47381778575722 35.70912083789317 20.350428981465978 40-41 19.566591762049974 24.437728559689372 35.544642242828566 20.45103743543209 42-43 19.99392455544103 24.075636688415372 34.67417183122727 21.256266924916332 44-45 19.708834663578628 24.439946824807475 34.43067751634462 21.420540995269278 46-47 20.825452133149334 24.385136564917506 32.514251070696474 22.275160231236686 48-49 20.67533499326249 25.28773980480687 30.791858904290976 23.245066297639664 50-51 20.8055895613747 25.508864808585823 30.31989126215609 23.36565436788339 52-53 20.717395763286138 25.648242145410833 30.20430629587415 23.430055795428878 54-55 20.814304000249255 25.573736015225197 29.909697754906517 23.702262229619034 56-57 21.250739959912345 25.625201217169145 28.952684107218897 24.171374715699613 58-59 20.648242628177666 25.35200662600708 30.313592921181154 23.6861578246341 60-61 21.22526817021133 26.50727691211235 29.156200142020193 23.11125477565613 62-63 21.858764147342676 26.356314407895248 29.664566148157956 22.120355296604117 64-65 21.739621495169377 25.916468257574383 30.03930966617941 22.304600581076837 66-67 21.738018522316874 26.416500284299648 29.49887967763795 22.346601515745526 68-69 21.975552572614 25.964486840294636 29.790369022180798 22.269591564910566 70-71 21.742432405415617 25.900731022431785 29.899739189475145 22.457097382677453 72-73 21.470438940291086 25.9566888705119 29.52276229509261 23.050109894104406 74-75 20.67767170270825 26.3966382540107 29.699057786824596 23.22663225645646 76-77 20.423238249043553 26.00769311656902 30.523082595200044 23.04598603918738 78-79 20.237957692083004 25.795236951597094 30.666164700995058 23.300640655324838 80-81 20.446390553438086 26.30168035808867 30.023808534723596 23.228120553749648 82-83 20.56130940171828 25.50194338353849 29.943684717501878 23.993062497241354 84-85 20.12672754227497 25.208810952416805 30.174967610610736 24.489493894697485 86-87 20.409739657844927 25.728358263208595 29.57338366909258 24.2885184098539 88-89 20.357712684818438 25.144524804519897 30.07946430078296 24.418298209878706 90-91 20.040634310677216 25.113042575150757 30.12170819891339 24.724614915258638 92-93 20.279405254459746 25.400796334834517 29.509956781893315 24.809841628812418 94-95 19.898972589401858 24.89971257429059 29.90655418896358 25.29476064734397 96-97 20.236922218111243 24.900849674475364 29.765609279561726 25.096618827851668 98-99 19.764371397413928 25.05842031469076 29.552110920704163 25.625097367191152 100-101 19.84899115393503 24.884393588874783 29.756869526383706 25.509745730806483 102-103 20.326390619807245 24.542258640744432 29.44915254237288 25.68219819707544 104-105 19.837779174044666 25.155132752093966 29.43017598155543 25.576912092305935 106-107 19.92693888466262 24.768341221891333 29.145517282562693 26.15920261088335 108-109 19.712387312533757 24.910810726600474 28.807745835237426 26.56905612562835 110-111 19.903804337243038 25.22533282271308 28.73744815365342 26.133414686390456 112-113 19.685230527160325 25.1709123199676 29.009007137682758 26.134850015189322 114-115 19.401828345324116 25.366538840005504 28.752690461292406 26.47894235337797 116-117 19.272295807029092 25.320616424752018 28.908508619223618 26.498579148995276 118-119 19.11331341051478 25.137932198373115 28.95018343359652 26.798570957515583 120-121 18.9298130372809 25.428461640318105 28.83564364661683 26.80608167578416 122-123 18.9880631697834 25.556370528556872 28.539474623688022 26.916091677971714 124-125 18.933303548468373 25.55671289381609 28.33112111443882 27.178862443276714 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 867.0 1 597.5 2 510.0 3 604.0 4 315.0 5 198.0 6 455.0 7 1157.0 8 1896.5 9 2024.5 10 1326.5 11 598.5 12 491.5 13 516.0 14 558.5 15 599.0 16 622.5 17 694.0 18 772.5 19 816.5 20 877.5 21 919.5 22 1004.5 23 1129.5 24 1283.5 25 1590.0 26 1833.0 27 2067.0 28 2511.0 29 3119.0 30 4114.5 31 5187.0 32 6834.0 33 8521.0 34 9528.5 35 10771.0 36 12936.5 37 14661.0 38 14588.5 39 14644.0 40 14956.5 41 14329.0 42 13018.5 43 13180.0 44 13830.0 45 13891.0 46 13518.5 47 12889.0 48 12649.5 49 11834.5 50 10824.0 51 9712.5 52 9226.0 53 9381.0 54 9278.0 55 8881.5 56 8539.5 57 8110.5 58 7375.0 59 6576.0 60 5806.0 61 5166.5 62 4418.0 63 3321.5 64 2471.5 65 2041.0 66 1607.5 67 1291.5 68 1005.5 69 700.0 70 524.5 71 354.5 72 278.5 73 289.5 74 204.0 75 140.0 76 110.5 77 54.5 78 37.0 79 15.5 80 4.0 81 3.0 82 1.5 83 1.5 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02414599760617099 2 0.00285597821148259 3 0.0012981719143102682 4 0.08100592745296074 5 0.004673418891516966 6 0.024924900754757152 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.192687657241073E-4 26-27 0.0 28-29 5.192687657241073E-4 30-31 0.00571195642296518 32-33 0.0 34-35 0.0 36-37 7.78903148586161E-4 38-39 1.2981719143102683E-4 40-41 1.2981719143102683E-4 42-43 0.0 44-45 0.003894515742930805 46-47 0.0027261610200515632 48-49 0.0 50-51 0.0027261610200515632 52-53 0.0 54-55 0.001557806297172322 56-57 2.5963438286205365E-4 58-59 0.0020770750628964292 60-61 1.2981719143102683E-4 62-63 0.0036348813600687513 64-65 0.0025963438286205364 66-67 0.0 68-69 0.0010385375314482146 70-71 0.002985795402913617 72-73 0.0035050641686377245 74-75 0.0 76-77 0.0019472578714654024 78-79 0.0011683547228792414 80-81 2.5963438286205365E-4 82-83 0.0010385375314482146 84-85 0.0 86-87 0.0025963438286205364 88-89 0.004024332934361831 90-91 0.003764698551499778 92-93 0.004543601700085939 94-95 0.0036348813600687513 96-97 0.0024665266371895096 98-99 0.004413784508654912 100-101 0.0036348813600687513 102-103 0.0012981719143102682 104-105 7.78903148586161E-4 106-107 0.0 108-109 0.0054523220401031264 110-111 0.0011683547228792414 112-113 0.004673418891516966 114-115 0.0 116-117 1.2981719143102683E-4 118-119 0.0010385375314482146 120-121 0.0 122-123 0.0011683547228792414 124-125 0.045176382617997335 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 385157.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.542088490089963 #Duplication Level Percentage of deduplicated Percentage of total 1 62.502337742613925 19.71454267915018 2 13.719622830441486 8.654911147368876 3 6.747567025830355 6.384970686646625 4 4.07731120302164 5.14427643069375 5 2.7343459839981663 4.312349149489613 6 1.8876743897377102 3.572471558495005 7 1.4529007860961478 3.2079267613656177 8 1.0531397823076825 2.6574582564786406 9 0.7976695373907825 2.2644146820806266 >10 4.623654221681805 26.243087266635595 >50 0.25792253780313 5.620194555712789 >100 0.13431748773773228 7.13603299852447 >500 0.008240336671026518 1.7341690827228289 >1k 0.0032961346684106077 3.353194744635374 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4526 1.1751052168336549 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3968 1.0302292311966288 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2474 0.6423354632007207 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 1933 0.5018732620723497 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 923 0.23964253538167551 RNA PCR Primer, Index 31 (95% over 22bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 780 0.20251481863240184 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 739 0.19186980893505765 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 702 0.18226333676916168 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 690 0.17914772417481703 No Hit ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT 632 0.1640889299688179 RNA PCR Primer, Index 31 (95% over 24bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 571 0.14825123261423262 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 570 0.1479915982313706 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 561 0.1456548887856121 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 504 0.13085572896247505 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 499 0.12955755704816477 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 458 0.11891254735082057 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 421 0.10930607518492459 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.5963438286205365E-4 0.0 10-11 0.0 0.0 0.0 2.5963438286205365E-4 0.0 12-13 0.0 0.0 0.0 2.5963438286205365E-4 0.0 14-15 0.0 0.0 0.0 2.5963438286205365E-4 0.0 16-17 0.0 0.0 0.0 6.490859571551342E-4 0.0 18-19 0.0 0.0 0.0 0.001557806297172322 0.0 20-21 0.0 0.0 0.0 0.0020770750628964292 0.0 22-23 0.0 0.0 0.0 0.0037646985514997776 0.0 24-25 0.0 0.0 0.0 0.009866106548758038 0.0 26-27 0.0 0.0 0.0 0.01960239590608505 0.0 28-29 0.0 0.0 0.0 0.037127716749273676 0.0 30-31 0.0 0.0 0.0 0.08723715264165002 0.0 32-33 0.0 0.0 0.0 0.15785770478012862 0.0 34-35 0.0 0.0 0.0 0.24314759955031326 0.0 36-37 0.0 0.0 0.0 0.3799749193186155 0.0 38-39 0.0 0.0 0.0 0.585605350545362 0.0 40-41 0.0 0.0 0.0 0.8357630784329507 0.0 42-43 0.0 0.0 0.0 1.045677476976921 0.0 44-45 0.0 0.0 0.0 1.2541638864151503 0.0 46-47 0.0 0.0 0.0 1.495104593711136 0.0 48-49 0.0 0.0 0.0 1.7782358882222056 0.0 50-51 0.0 0.0 0.0 2.0742190846849464 0.0 52-53 0.0 0.0 0.0 2.356182024473137 0.0 54-55 0.0 0.0 0.0 2.601406699086347 0.0 56-57 0.0 0.0 0.0 2.8971302611662257 0.0 58-59 0.0 0.0 0.0 3.222452142892379 0.0 60-61 0.0 0.0 0.0 3.600220169956667 0.0 62-63 0.0 0.0 0.0 3.967862456089335 0.0 64-65 0.0 0.0 0.0 4.32511936690752 0.0 66-67 0.0 0.0 0.0 4.708988801969067 0.0 68-69 0.0 0.0 0.0 5.134399738288542 0.0 70-71 0.0 0.0 0.0 5.649514353886857 0.0 72-73 0.0 0.0 0.0 6.157878475530758 0.0 74-75 0.0 0.0 0.0 6.657025576583056 0.0 76-77 0.0 0.0 0.0 7.172789278138525 0.0 78-79 0.0 0.0 0.0 7.776958487058524 0.0 80-81 0.0 0.0 0.0 8.440454152462502 0.0 82-83 0.0 0.0 0.0 9.165612983796219 0.0 84-85 0.0 0.0 0.0 9.837676583834643 0.0 86-87 0.0 0.0 0.0 10.526616418759104 0.0 88-89 0.0 0.0 0.0 11.22775906967808 0.0 90-91 0.0 0.0 0.0 12.068195567002547 0.0 92-93 0.0 0.0 0.0 12.95731351111365 0.0 94-95 0.0 0.0 0.0 13.856427378964941 0.0 96-97 0.0 0.0 0.0 14.767224794045024 0.0 98-99 0.0 0.0 0.0 15.682435993633765 0.0 100-101 0.0 0.0 0.0 16.696697710284376 0.0 102-103 0.0 0.0 0.0 17.762237217550243 0.0 104-105 0.0 0.0 0.0 18.832320326516196 0.0 106-107 0.0 0.0 0.0 19.925510895556876 0.0 108-109 0.0 0.0 0.0 20.966774588025142 0.0 110-111 0.0 0.0 0.0 22.096313970666507 0.0 112-113 0.0 0.0 0.0 23.27765041268885 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1580 0.0 92.34043 1 GTGGTAT 295 0.0 74.690025 1 GTATCAA 3385 0.0 62.80474 1 ATCAACG 4820 0.0 43.971676 3 TCAACGC 4850 0.0 43.699688 4 TATCAAC 4875 0.0 43.475582 2 CAACGCA 4920 0.0 42.934628 5 AGGCTTA 170 0.0 42.002663 6 AACGCAG 5030 0.0 41.995697 6 TGGTATC 540 0.0 41.89478 2 CGCAGAG 5655 0.0 37.349415 8 GATTCGT 40 1.1975727E-4 37.185024 70-71 ACGCAGA 5695 0.0 37.087086 7 GTACATG 2600 0.0 36.875286 1 GGGTATA 65 0.001929302 36.64625 1 GTGTAAG 65 0.001929302 36.64625 1 ATATAGG 180 1.4551915E-11 36.38231 2 GTGGTAC 100 1.0542484E-5 35.730095 1 GCAGAGT 6040 0.0 34.47618 9 ACATGGG 2760 0.0 34.08145 3 >>END_MODULE