FastQCFastQC Report
Fri 27 May 2016
SRR940167_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940167_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1860782
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG27730.14902336759491439No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG26680.14338057870293244No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC21660.11640267371459956No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18900.069.296261
GTATCAA39100.056.0299151
ATCAACG47950.045.5644763
TCAACGC48300.045.357554
CAACGCA50450.043.3065725
AACGCAG52000.041.8843236
GTACATG123700.040.3295361
TATCAAC54500.040.306842
TACATGG122650.039.752582
ACATGGG128000.036.742273
AATACCG1001.0586593E-535.719075
ACGCAGA65900.032.9524577
CATGGGG60200.032.732594
CGCAGAG66650.032.404868
CATGGGA73850.030.229414
ATGGGGA34450.027.4762085
GCAGAGT75000.026.972519
CCAAGAC18200.025.5136223
ATGGGAT30750.025.167915
ACTTGAC12600.025.0270168
Produced by FastQC (version 0.11.5)