FastQCFastQC Report
Fri 27 May 2016
SRR940167_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940167_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1860782
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54630.29358624492283353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43580.23420260944054705No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG32390.17406660210599628No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG26820.1441329505551967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23830.12806443742469564No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC20870.11215714683396551No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36100.084.34771
GTATCAA75850.059.784511
ATCAACG96900.046.790973
TCAACGC98450.046.051814
CAACGCA100150.045.2772035
TATCAAC100850.045.0764582
AACGCAG102450.044.3745086
ACGCAGA120200.037.763067
CGCAGAG122000.037.2059028
GTACATG116650.035.706891
TACATGG116400.034.857422
GCAGAGT133800.033.3911179
ACATGGG119350.032.9475143
TGGTATC13300.031.3579392
GAGTACT73900.029.46346312-13
TAAGGTG6650.028.6354395
CATGGGG58650.028.0361774
GTAAGGT5800.027.7340644
CAGAGTA132100.027.5610510-11
CATGGGA67800.027.3280374
Produced by FastQC (version 0.11.5)