FastQCFastQC Report
Fri 27 May 2016
SRR940166_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940166_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091313
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG22070.1055317879246196No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG22000.1051970699747001No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32350.055.0096361
GGTATCA21450.048.834561
TCAACGC38650.044.5030944
ATCAACG39400.043.6559563
AACGCAG41800.041.7069636
CAACGCA41700.041.2470865
TATCAAC44350.039.0518152
GTACATG91650.037.3402521
TACATGG94500.035.206422
ACATGGG97150.033.3271373
ACGCAGA52350.033.0705647
CGCAGAG55850.031.0003268
CATGGGG64750.029.7815364
GCAGAGT66750.024.7793549
ATGGGGA33300.024.4854355
CATGGGA44300.024.4517274
CGATACC3750.023.81368118-119
GAGTACA74300.022.4290621
AGTACAT70800.021.5203132
GTAATAC4700.020.2611473
Produced by FastQC (version 0.11.5)