FastQCFastQC Report
Fri 27 May 2016
SRR940166_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940166_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091313
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36200.17309699695837016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29470.14091625691610962No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG25510.12198078432066363No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG22430.1072531945242056No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34200.067.92681
GTATCAA57600.057.808541
TCAACGC75900.043.5440864
ATCAACG76550.043.253173
CAACGCA79100.041.8307575
AACGCAG80350.041.1780326
TATCAAC80850.041.101052
GTACATG89250.035.4396131
ACGCAGA94700.034.991917
TACATGG90300.034.359652
CGCAGAG97800.033.7610978
ACATGGG93200.032.3312873
GTGGTAT15600.030.1651271
GCAGAGT112500.028.8737059
CATGGGG58100.027.776094
GAGTACT62000.026.67568212-13
CATGGGA43250.025.3343434
AGAGTAC106500.024.91424210-11
TGGTATC18000.024.813472
CAGAGTA109700.024.72980110-11
Produced by FastQC (version 0.11.5)