FastQCFastQC Report
Fri 27 May 2016
SRR940165_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940165_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715574
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60860.8505060273291093No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21990.3073057433612736No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19230.26873530899669357No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10080.14086593420107493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11800.080.721861
CGATCCG150.004237605759.49701370-71
GTATCAA25450.059.4154171
TATAGCG453.1627077E-452.9139675
TCAACGC29900.048.3791164
ATCAACG30600.047.6614763
CAACGCA31200.046.363325
AACGCAG32350.044.5062566
TATCAAC35100.042.3990142
GTACATG71350.041.9688641
TACATGG73000.039.7126582
GAACAAA11400.038.643821
CGACCGT401.19797114E-437.18563518-19
ACATGGG75800.036.59653
CGCAGAG39350.036.2866178
ACGCAGA39650.036.1621177
CATGGGA57200.034.135064
CATGGGG16600.033.3501474
TCGACCG452.39399E-433.05389818-19
GCAGAGT41600.032.7508169
Produced by FastQC (version 0.11.5)