FastQCFastQC Report
Fri 27 May 2016
SRR940165_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940165_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715574
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113961.5925676449954862No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84321.1783547194280395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63260.8840455354722223No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26660.3725680362897478No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22570.3154111244958593No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18470.2581144647513744No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16680.23309958159463592No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13120.1833493111823515No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8390.11724853055029949No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7540.10536995474961361No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT7160.10005953262695402No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28300.090.564911
GTATCAA64400.064.232011
TATAGCG300.00423349659.508535
ATCAACG87100.047.266723
TCAACGC87450.047.077544
TATCAAC88150.046.7745632
TGGTATC9200.045.982912
CAACGCA89650.045.8677025
AACGCAG91850.044.898656
GTGGTAT10600.039.9236681
ACGCAGA104000.039.6421977
CGCAGAG104800.039.2828188
GTACATG66800.038.1896131
GCAGAGT111500.036.70899
TACATGG69600.036.4692082
ACATGGG69450.035.0870323
CGTATAG1209.155556E-734.7692031
CATGGGA50850.033.392544
CGCGATA452.3942163E-433.053364104-105
CATATAG2600.032.0811733
Produced by FastQC (version 0.11.5)