##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940165_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 715574 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.72070813081526 34.0 31.0 34.0 31.0 34.0 2 32.746288993171916 34.0 31.0 34.0 31.0 34.0 3 32.80966608624684 34.0 31.0 34.0 31.0 34.0 4 36.25265451232158 37.0 35.0 37.0 35.0 37.0 5 36.23763300511198 37.0 37.0 37.0 35.0 37.0 6 36.315930986872075 37.0 37.0 37.0 35.0 37.0 7 36.28639246255454 37.0 37.0 37.0 35.0 37.0 8 36.28571608247365 37.0 37.0 37.0 35.0 37.0 9 38.08385436027581 39.0 39.0 39.0 35.0 39.0 10-11 38.065986886052315 39.0 39.0 39.0 35.0 39.0 12-13 38.05882689980352 39.0 39.0 39.0 35.0 39.0 14-15 39.463603345006945 41.0 39.0 41.0 36.0 41.0 16-17 39.48187538954741 41.0 39.0 41.0 36.5 41.0 18-19 39.59774670404458 41.0 40.0 41.0 37.0 41.0 20-21 39.59598518112732 41.0 40.0 41.0 37.0 41.0 22-23 39.533631043050754 41.0 40.0 41.0 36.0 41.0 24-25 39.49380357587056 41.0 39.5 41.0 36.0 41.0 26-27 39.34832665803956 41.0 39.0 41.0 35.5 41.0 28-29 39.2201889671788 41.0 39.0 41.0 35.0 41.0 30-31 38.92760427293334 41.0 38.5 41.0 35.0 41.0 32-33 38.5885743473072 40.0 38.0 41.0 35.0 41.0 34-35 38.39128867175163 40.0 37.5 41.0 35.0 41.0 36-37 38.21560593313899 40.0 37.0 41.0 35.0 41.0 38-39 38.02694340487497 40.0 37.0 41.0 34.0 41.0 40-41 37.86384007803525 40.0 37.0 41.0 34.0 41.0 42-43 37.61749308946384 40.0 37.0 41.0 33.0 41.0 44-45 37.50708801605424 40.0 37.0 41.0 33.0 41.0 46-47 37.01390911352285 40.0 37.0 41.0 32.0 41.0 48-49 36.53844116751028 40.0 36.0 41.0 30.0 41.0 50-51 36.340404346720256 40.0 36.0 41.0 29.5 41.0 52-53 36.24830974853754 40.0 35.5 41.0 30.0 41.0 54-55 36.10166593531906 40.0 35.0 41.0 29.0 41.0 56-57 35.937956801113515 40.0 35.0 41.0 29.0 41.0 58-59 35.716827330227204 39.0 35.0 41.0 28.0 41.0 60-61 35.495277916749345 39.0 35.0 41.0 28.0 41.0 62-63 35.26338296248886 39.0 35.0 41.0 28.0 41.0 64-65 34.98694474645529 38.5 35.0 41.0 27.5 41.0 66-67 34.680230835664794 37.0 35.0 40.5 26.5 41.0 68-69 34.3432866482013 37.0 35.0 40.0 26.0 41.0 70-71 34.07598026199946 36.5 35.0 39.0 26.0 41.0 72-73 33.87284194227291 36.0 35.0 39.0 26.0 41.0 74-75 33.552878528286385 36.0 35.0 39.0 26.0 41.0 76-77 33.21699852146668 35.0 35.0 37.5 26.0 39.5 78-79 32.88391766609743 35.0 34.5 37.0 25.5 39.0 80-81 32.582156422676064 35.0 34.0 37.0 25.0 39.0 82-83 32.305414953589704 35.0 34.0 36.0 25.0 38.0 84-85 32.05807924826782 35.0 34.0 36.0 24.0 37.0 86-87 31.845384963679507 35.0 34.0 35.5 23.5 37.0 88-89 31.665820026999306 35.0 34.0 35.0 23.0 36.5 90-91 31.519490646669666 35.0 34.0 35.0 21.5 36.0 92-93 31.374590189134878 35.0 34.0 35.0 20.0 36.0 94-95 31.252398074832232 35.0 34.0 35.0 19.5 36.0 96-97 31.159107234192412 35.0 34.0 35.0 19.0 36.0 98-99 31.073234913509992 35.0 34.0 35.0 18.0 35.0 100-101 31.0267288917708 35.0 34.0 35.0 17.5 35.0 102-103 30.279965454306613 34.5 32.0 35.0 11.5 35.0 104-105 30.8384345993566 35.0 33.5 35.0 11.5 35.0 106-107 30.86912953796533 35.0 34.0 35.0 7.5 35.0 108-109 30.845358411568895 35.0 34.0 35.0 6.0 35.0 110-111 30.786062657391128 35.0 34.0 35.0 2.0 35.0 112-113 30.71192846581905 35.0 34.0 35.0 2.0 35.0 114-115 30.62213901008142 35.0 34.0 35.0 2.0 35.0 116-117 30.526712122016733 35.0 33.0 35.0 2.0 35.0 118-119 30.42935531475431 35.0 33.0 35.0 2.0 35.0 120-121 30.297280225385492 35.0 33.0 35.0 2.0 35.0 122-123 30.126038676642807 35.0 33.0 35.0 2.0 35.0 124-125 28.543500742061617 33.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22093290813273114 1101 2 0.21968805098088495 1101 3 0.18198006659910249 1101 4 0.07581183500438016 1101 5 0.04656374087740289 1101 6 0.10703439823613792 1101 7 0.16290022239054025 1101 8 0.0907404255469686 1101 9 0.08633216633216989 1101 10-11 0.10817791054028447 1101 12-13 0.14191570039667312 1101 14-15 0.21986340654967051 1101 16-17 0.17026251278718973 1101 18-19 0.17865109577643068 1101 20-21 0.24522837008304066 1101 22-23 0.22482109397089545 1101 24-25 0.1775991750845236 1101 26-27 0.2841737009814196 1101 28-29 0.2989355283661723 1101 30-31 0.3434723667469015 1101 32-33 0.3591443771573779 1101 34-35 0.2852143584255771 1101 36-37 0.4079509720065957 1101 38-39 0.4557799981146502 1101 40-41 0.4389765880240688 1101 42-43 0.36138337591857095 1101 44-45 0.3961280676131409 1101 46-47 0.46450953463793354 1101 48-49 0.37305202272142424 1101 50-51 0.47085834219466705 1101 52-53 0.4958025529790504 1101 54-55 0.5634316577811234 1101 56-57 0.36724107445140675 1101 58-59 0.42828542238135725 1101 60-61 0.432335831106478 1101 62-63 0.37130518004138224 1101 64-65 0.44240166462939357 1101 66-67 0.3318993058343267 1101 68-69 0.4166688793450035 1101 70-71 0.4718550646207902 1101 72-73 0.34869767549124475 1101 74-75 0.32233567451220324 1101 76-77 0.3890482951353462 1101 78-79 0.4047737808980614 1101 80-81 0.35250323866036837 1101 82-83 0.32960472694563236 1101 84-85 0.3507842781586561 1101 86-87 0.3285572654963467 1101 88-89 0.2898558316397626 1101 90-91 0.29478128806023207 1101 92-93 0.28050905380946745 1101 94-95 0.22563430329919498 1101 96-97 0.33403076710544966 1101 98-99 0.26289944495799844 1101 100-101 0.3265194395261872 1101 102-103 0.32120909380380525 1101 104-105 0.3565375352149758 1101 106-107 0.3601055995613791 1101 108-109 0.30216614008169884 1101 110-111 0.3195910727984632 1101 112-113 0.2632804785708487 1101 114-115 0.26166526689321756 1101 116-117 0.21027269969805218 1101 118-119 0.15341156204331696 1101 120-121 0.14996659093645448 1101 122-123 0.09301220860128012 1101 124-125 0.15856122184478494 1102 1 0.055186736741397624 1102 2 0.16712614156413252 1102 3 0.14361048367306495 1102 4 0.05224552676065031 1102 5 -0.01345784595869759 1102 6 0.051334251608572856 1102 7 0.1315097043064739 1102 8 0.12787589312598158 1102 9 0.10994531393725282 1102 10-11 0.1469344273209927 1102 12-13 0.12062415591964282 1102 14-15 0.20561405822773793 1102 16-17 0.22111580376144957 1102 18-19 0.1994426809833385 1102 20-21 0.2383898233251429 1102 22-23 0.2360045006180158 1102 24-25 0.2997042989033645 1102 26-27 0.23345685510329162 1102 28-29 0.21513991090120044 1102 30-31 0.2996317427651505 1102 32-33 0.3227705583238816 1102 34-35 0.31747038644773795 1102 36-37 0.3214203431372624 1102 38-39 0.307359299189919 1102 40-41 0.18765693993022836 1102 42-43 0.285690967968101 1102 44-45 0.25260208195005873 1102 46-47 0.35902525637140315 1102 48-49 0.28970545217727306 1102 50-51 0.009503870053912067 1102 52-53 0.21113226135311436 1102 54-55 0.21948411786583222 1102 56-57 0.06902606141784418 1102 58-59 0.08532660028946992 1102 60-61 0.1035998829148852 1102 62-63 0.08792195260573266 1102 64-65 0.10808572295457708 1102 66-67 0.18765851078708806 1102 68-69 0.20324838406966705 1102 70-71 0.15696625719166235 1102 72-73 0.13163898373497318 1102 74-75 0.3571128820716609 1102 76-77 0.3257927558717384 1102 78-79 0.4459160503669466 1102 80-81 0.38483665345346907 1102 82-83 0.29923754708248396 1102 84-85 0.23360034202310231 1102 86-87 0.22857966693003462 1102 88-89 0.2283109473126217 1102 90-91 0.36627444544048515 1102 92-93 0.2915621248423754 1102 94-95 0.3863482980857711 1102 96-97 0.3710246983534127 1102 98-99 0.4374514162841585 1102 100-101 0.3309233577405877 1102 102-103 0.3745978197731752 1102 104-105 0.45705378643686956 1102 106-107 0.2541284897536258 1102 108-109 0.1915000029034637 1102 110-111 0.33005152408552263 1102 112-113 0.45429648541346523 1102 114-115 0.386305825707165 1102 116-117 0.30937867884435377 1102 118-119 0.31430375005244215 1102 120-121 0.40250304417855176 1102 122-123 0.2753731160629904 1102 124-125 0.39993259688551674 1103 1 0.08881715860711381 1103 2 0.05039552533202141 1103 3 -0.01151706297990529 1103 4 -0.07541593330360286 1103 5 0.02743865645325627 1103 6 0.05946440659814556 1103 7 -0.036579966076317305 1103 8 -0.008165477711003177 1103 9 -0.032385270068303385 1103 10-11 -0.116676759321102 1103 12-13 0.0413997080511308 1103 14-15 0.0168102675794799 1103 16-17 0.1559094027624255 1103 18-19 0.011941044478703589 1103 20-21 0.09915248483556383 1103 22-23 0.2047376949743409 1103 24-25 0.21188392165127823 1103 26-27 0.0522726300008145 1103 28-29 0.10777614361719401 1103 30-31 0.1707028728093647 1103 32-33 0.05461341359644223 1103 34-35 0.17117241168514852 1103 36-37 0.04868803501113206 1103 38-39 0.0870369329552858 1103 40-41 0.2801385859014047 1103 42-43 0.14617416484212953 1103 44-45 0.16781200772827987 1103 46-47 5.134181486923239E-4 1103 48-49 -0.038640822131114305 1103 50-51 0.10179064608943378 1103 52-53 0.10974047710988089 1103 54-55 0.08914886101014474 1103 56-57 0.20401150911516197 1103 58-59 0.10117753234180071 1103 60-61 0.19327026570590533 1103 62-63 0.2636498195100714 1103 64-65 0.17305999206643463 1103 66-67 0.1453500825364813 1103 68-69 0.1704820366472859 1103 70-71 0.3233700760381524 1103 72-73 0.1713642523735004 1103 74-75 0.16818622769137193 1103 76-77 0.028556182762791593 1103 78-79 0.1973763160345925 1103 80-81 0.2313113707158223 1103 82-83 0.19710153088864502 1103 84-85 0.21176785967218592 1103 86-87 0.1452352557192178 1103 88-89 0.0506852385802361 1103 90-91 0.1170342871275416 1103 92-93 0.09214018863081108 1103 94-95 0.22255583708493276 1103 96-97 0.3667506518061856 1103 98-99 0.36216616093908627 1103 100-101 0.2993715485217834 1103 102-103 0.2514667080578157 1103 104-105 0.28195030899042095 1103 106-107 0.2771498297739683 1103 108-109 0.2967594770064075 1103 110-111 0.21993289008852557 1103 112-113 0.31853256505333505 1103 114-115 0.3749076968613778 1103 116-117 0.2592437301550703 1103 118-119 0.2744903619339709 1103 120-121 0.4435249280219722 1103 122-123 0.48139192831311206 1103 124-125 0.3743965786775121 1104 1 -0.028771659098183022 1104 2 -0.025533124362461024 1104 3 0.0927453971644141 1104 4 0.07875817802577956 1104 5 -0.16651315148925505 1104 6 0.01821093927733841 1104 7 -0.13839728639259619 1104 8 -0.015078402303558391 1104 9 0.05029934934079705 1104 10-11 0.14993972785104148 1104 12-13 0.10895298880293325 1104 14-15 0.12725122194410687 1104 16-17 0.15877456963804093 1104 18-19 0.1219649332085595 1104 20-21 0.11347733207601607 1104 22-23 0.17368826899485157 1104 24-25 0.14348367684115715 1104 26-27 0.2637648859063688 1104 28-29 0.22232062897300153 1104 30-31 0.1797297525661321 1104 32-33 0.13101638290633844 1104 34-35 0.23828246765584993 1104 36-37 0.3490281039133407 1104 38-39 0.5359071539754012 1104 40-41 0.5001231865548945 1104 42-43 0.5528876876400659 1104 44-45 0.5825800797498388 1104 46-47 0.4524946033060999 1104 48-49 0.5038618678188342 1104 50-51 0.4908270023671406 1104 52-53 0.4665077989068749 1104 54-55 0.45013718317237306 1104 56-57 0.3633754963613427 1104 58-59 0.33547661529097184 1104 60-61 0.2407886017867753 1104 62-63 0.37273543395386355 1104 64-65 0.4273076451467972 1104 66-67 0.42069681461747166 1104 68-69 0.37268532776403873 1104 70-71 0.35864642520846957 1104 72-73 0.39027484732132933 1104 74-75 0.37791496227968935 1104 76-77 0.4629164682429803 1104 78-79 0.48489494825673063 1104 80-81 0.35884905469358586 1104 82-83 0.4078934126690399 1104 84-85 0.422674249413852 1104 86-87 0.3154883577991221 1104 88-89 0.42487060327822235 1104 90-91 0.36655947394333666 1104 92-93 0.2834963182617187 1104 94-95 0.34695935919187804 1104 96-97 0.3547080666902467 1104 98-99 0.4166043315756873 1104 100-101 0.3673720026050731 1104 102-103 0.45369072906410324 1104 104-105 0.3252506061188356 1104 106-107 0.39303238014266384 1104 108-109 0.356656518555031 1104 110-111 0.3213706559987166 1104 112-113 0.39996105187011466 1104 114-115 0.42003419854444957 1104 116-117 0.3097437153480058 1104 118-119 0.29742206188362275 1104 120-121 0.44244203807794236 1104 122-123 0.42287786653804105 1104 124-125 0.6237899826240891 1105 1 -0.10689638636033294 1105 2 -0.12259773777776672 1105 3 -0.027818794416347714 1105 4 -0.036534536358296066 1105 5 -0.4014187478275346 1105 6 0.01651099455220617 1105 7 -0.22533593128548546 1105 8 -0.14929839629120778 1105 9 -0.10707305783136434 1105 10-11 0.02360416089572226 1105 12-13 -0.02518506261239395 1105 14-15 -0.027149918463763356 1105 16-17 0.007677799823937903 1105 18-19 -0.02748328781335374 1105 20-21 0.055627330621419446 1105 22-23 -0.021298203207699373 1105 24-25 -0.08593564609016369 1105 26-27 -0.010840674551737095 1105 28-29 -0.016063405316806723 1105 30-31 -0.1606097515116076 1105 32-33 -0.06284762151565815 1105 34-35 0.018529459054306585 1105 36-37 -0.0179577670537725 1105 38-39 0.09101910983308414 1105 40-41 0.1970016109301369 1105 42-43 0.15625557584906602 1105 44-45 0.21230389934041938 1105 46-47 0.1892664538505997 1105 48-49 -0.08807430425086693 1105 50-51 -0.028658359489320162 1105 52-53 -0.008906520193988854 1105 54-55 0.12010838205523555 1105 56-57 0.11197810882630677 1105 58-59 0.07194251457433865 1105 60-61 0.15050873638593032 1105 62-63 -0.05037334117437808 1105 64-65 -0.00901327881631886 1105 66-67 0.05087737919694746 1105 68-69 -0.029314264947437607 1105 70-71 -0.043102006245845814 1105 72-73 0.10053657868545685 1105 74-75 0.11569824599493472 1105 76-77 0.020324755594735677 1105 78-79 -0.04341160423985002 1105 80-81 0.027188675139477425 1105 82-83 -0.08648649055145086 1105 84-85 0.008644614324971656 1105 86-87 0.046410769335086144 1105 88-89 -0.048560608599089505 1105 90-91 -0.08845502358868984 1105 92-93 -0.152318404248728 1105 94-95 -0.07460996228483197 1105 96-97 -0.11386138085196151 1105 98-99 -0.0041853232957720365 1105 100-101 0.017539766688045688 1105 102-103 -0.06972642166174481 1105 104-105 0.051042151972737315 1105 106-107 0.03547426879774207 1105 108-109 0.02019983175020812 1105 110-111 -0.033650629675396004 1105 112-113 -0.0354803228256344 1105 114-115 0.03642998601741709 1105 116-117 0.013978546274781678 1105 118-119 0.05193520663590334 1105 120-121 -0.058320173304799994 1105 122-123 0.029554004279141566 1105 124-125 0.28428493515558273 1106 1 0.06606144123312419 1106 2 0.09469974998880559 1106 3 0.11979788964770677 1106 4 0.023532868260758733 1106 5 0.023206671556884828 1106 6 0.02483521872655814 1106 7 0.14414671268816193 1106 8 0.0636162756748746 1106 9 0.16281443063616052 1106 10-11 0.04555897062638792 1106 12-13 0.08306774070365464 1106 14-15 -0.029900663457198107 1106 16-17 0.008521778401465951 1106 18-19 0.09619746284450059 1106 20-21 0.2028428643739275 1106 22-23 0.017371360708274608 1106 24-25 0.1881741033306099 1106 26-27 0.14239732361821922 1106 28-29 0.12351085437852305 1106 30-31 0.12775338471657705 1106 32-33 0.1944554927747646 1106 34-35 0.2764108549626698 1106 36-37 0.04912290062705438 1106 38-39 0.18756561769410496 1106 40-41 0.03127396120682846 1106 42-43 0.21887386072545922 1106 44-45 0.11218729579059072 1106 46-47 -0.051430363658688805 1106 48-49 1.0631631390367602E-5 1106 50-51 -0.10210154580682484 1106 52-53 -0.007780268197706164 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-0.5738986946983005 2216 36-37 -0.5768598566299232 2216 38-39 -0.5566431791248263 2216 40-41 -0.5301024382380675 2216 42-43 -0.6381609339507222 2216 44-45 -0.5380629325067758 2216 46-47 -0.5265348052109644 2216 48-49 -0.46128775123228394 2216 50-51 -0.36481177712419566 2216 52-53 -0.22864844534208828 2216 54-55 -0.33606768266737674 2216 56-57 -0.4048033103669155 2216 58-59 -0.24427236332436308 2216 60-61 -0.43649249063473405 2216 62-63 -0.4565994697967284 2216 64-65 -0.5373782100558273 2216 66-67 -0.39084023128042844 2216 68-69 -0.4018959965394018 2216 70-71 -0.4656202021977691 2216 72-73 -0.1535324330349539 2216 74-75 -0.12714043173555467 2216 76-77 -0.16079805934361246 2216 78-79 -0.2312815764589402 2216 80-81 -0.30309470473811473 2216 82-83 -0.3533252556661921 2216 84-85 -0.29335320835051704 2216 86-87 -0.2856912954821915 2216 88-89 -0.26066806211248306 2216 90-91 -0.3289041058718958 2216 92-93 -0.32291724130985244 2216 94-95 -0.31329497479170243 2216 96-97 -0.40045590695618927 2216 98-99 -0.3031516403040264 2216 100-101 -0.29821561563630183 2216 102-103 -0.3116005956073984 2216 104-105 -0.3068135110735497 2216 106-107 -0.18698712738429535 2216 108-109 -0.29393238791925924 2216 110-111 -0.4311947678299113 2216 112-113 -0.41901093826570346 2216 114-115 -0.4921027958241311 2216 116-117 -0.5499750818105191 2216 118-119 -0.3941694578631427 2216 120-121 -0.5429383586977927 2216 122-123 -0.5774957210065885 2216 124-125 -0.7940612448778417 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 2.0 4 18.0 5 70.0 6 169.0 7 354.0 8 623.0 9 1065.0 10 2238.0 11 3316.0 12 3779.0 13 5874.0 14 10697.0 15 11308.0 16 4095.0 17 3068.0 18 2931.0 19 2595.0 20 2522.0 21 2505.0 22 2561.0 23 2579.0 24 3009.0 25 3598.0 26 4255.0 27 5026.0 28 6109.0 29 7393.0 30 9109.0 31 11273.0 32 14451.0 33 19156.0 34 27711.0 35 53936.0 36 89442.0 37 239266.0 38 158225.0 39 1243.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.70615156441551 19.30529132110392 13.967002106575677 20.0215550079049 2 15.39861199231928 22.01414839887193 41.202250551317995 21.384989057490795 3 17.597151318060426 30.863003609765233 30.695442304547914 20.84440276762643 4 11.007376574550399 17.915677109121273 39.21948736023811 31.857458956090216 5 10.723489295097547 41.047990385152886 33.688859075409475 14.539661244340095 6 27.084975761947096 39.63816249280127 18.31776171226328 14.959100032988355 7 25.44894029129063 36.336703122248714 22.0351773541241 16.17917923233656 8 26.906790911911276 33.8641426323483 20.247521570096175 18.981544885644254 9 29.25986690405185 14.397253114283078 20.440513489869673 35.9023664917954 10-11 24.006182449334382 28.318454834859846 27.047866468038244 20.627496247767528 12-13 26.624430177731444 26.4331152333651 28.268564816496966 18.673889772406486 14-15 23.206265180121132 25.621389262326467 29.088186546744293 22.084159010808108 16-17 19.493790998555006 29.65605234399237 29.225628656155756 21.62452800129686 18-19 19.71864545106446 25.52664015182217 35.20886728696124 19.545847110152128 20-21 21.242946222193652 24.492924561261308 35.467610617490294 18.796518599054746 22-23 20.665647438280317 24.826013801507603 35.40877673028925 19.099562029922833 24-25 19.90990348898634 25.24861438357887 35.755122518398025 19.086359609036762 26-27 19.450888377721938 25.448101803587047 35.606030962555934 19.494978856135077 28-29 19.579971505253503 25.024159045125533 35.90345046365103 19.492418985969937 30-31 19.718849206210557 25.1081201386118 35.87254646423213 19.30048419094551 32-33 19.59126519409593 25.243440929938764 35.54419249441707 19.62110138154824 34-35 19.834426628133496 25.085595619740236 35.6829622093592 19.397015542767065 36-37 20.00673589828514 24.89990483098066 35.0058484925982 20.087510778136 38-39 19.89278529738734 25.13054214556576 34.9456065659223 20.031065991124603 40-41 20.036320543117196 25.001362544422438 35.060503959763714 19.901812952696652 42-43 19.890884800174405 25.123327566401237 34.643656700774486 20.342130932649873 44-45 19.939612505424183 25.23613103569235 34.3605682657497 20.46368819313377 46-47 20.834192224351884 25.634735020183648 32.918177441410826 20.61289531405365 48-49 20.502701327885024 26.126717851682706 32.21868038805211 21.15190043238016 50-51 20.88475317428208 26.202998928804266 31.81262669352692 21.09962120338674 52-53 20.759293070523157 26.306452097513393 31.871987992847693 21.062266839115757 54-55 20.447076728070833 26.68233738773645 31.72426611623492 21.1463197679578 56-57 20.694883680630767 26.49429198698106 31.541785394362282 21.26903893802589 58-59 20.453216221032 25.94575520314722 32.18072747981455 21.420301096006234 60-61 20.57120657151681 26.37382908522261 32.0355854678698 21.01937887539077 62-63 20.603188889059698 26.200375377159013 32.41953358759103 20.776902146190256 64-65 20.393316800723355 26.02355792108369 32.555126751174434 21.027998527018518 66-67 20.52768936760426 26.38768258981445 31.907877957858915 21.17675008472237 68-69 20.784426895035686 26.12222195825129 32.145487424657645 20.947863722055377 70-71 20.805103221586812 26.043256357365262 32.06318793428253 21.088452486765387 72-73 20.704478579778783 26.420497225562695 31.49756725765687 21.37745693700165 74-75 20.37329472563285 26.789682129311576 31.399757397557764 21.437265747497815 76-77 20.455950902755117 26.56236134102056 31.650547578503257 21.33114017772107 78-79 20.55372949525271 26.442284848637303 31.409331913478283 21.594653742631706 80-81 20.414297932283542 26.870042427596385 31.159268389484218 21.556391250635855 82-83 20.704883061019572 26.5616494926041 31.099319349521465 21.634148096854865 84-85 20.422765500143942 26.484053361357457 31.18112172884985 21.91205940964876 86-87 20.3762309681648 26.565783120406905 31.142158174648817 21.915827736779473 88-89 20.598628579574317 26.26284789503805 31.19202331680259 21.946500208585036 90-91 20.516955844228445 26.51407687953769 31.09987631632275 21.869090959911116 92-93 20.572666815274562 26.563335264304268 31.089913134259312 21.774084786161858 94-95 20.329720241605713 26.16295807676935 31.287715953970814 22.219605727654127 96-97 20.83211339136414 26.007648774106812 31.053142419111733 22.10709541541732 98-99 20.42007669774826 26.15437937434315 30.930868607589275 22.494675320319313 100-101 20.30921243418317 26.24532250682189 30.990632784903443 22.45483227409149 102-103 20.64278776613957 25.882007672366203 30.935427744897314 22.539776816596905 104-105 20.303622549906752 26.144157124599705 30.85662338352017 22.695596941973378 106-107 20.554477943580956 25.80851176817492 30.81756743537356 22.819442852870566 108-109 20.475796820613947 25.930884795647337 30.34280878611295 23.25050959762577 110-111 20.458063060009938 25.991937743560435 30.617987538370485 22.93201165805915 112-113 20.240294715305126 26.10762810640385 30.70885124200252 22.9432259362885 114-115 20.1785559564769 26.204557460164846 30.433050949307827 23.183835634050425 116-117 20.044370067323715 26.029857212232166 30.676206813425615 23.249565907018507 118-119 19.76032925031269 26.199087434404984 30.460405413903697 23.580177901378633 120-121 19.746720106432335 26.38679266377108 30.424122240669004 23.44236498912758 122-123 19.811726825014393 26.354541084135974 30.359328969360888 23.474403121488745 124-125 19.662631130460433 26.364571165733803 30.181798354608503 23.79099934919726 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1580.0 1 1107.0 2 1501.5 3 2051.0 4 1035.5 5 572.5 6 1523.0 7 3983.5 8 6797.0 9 7076.5 10 4335.5 11 1828.0 12 1244.5 13 1253.5 14 1307.5 15 1362.0 16 1487.5 17 1650.0 18 1790.0 19 1834.5 20 1957.0 21 2219.5 22 2463.5 23 2846.0 24 3381.0 25 4191.0 26 5054.0 27 5966.0 28 6780.0 29 8112.0 30 10048.0 31 11307.5 32 13448.5 33 15945.5 34 17688.0 35 18929.0 36 21659.0 37 24785.5 38 24834.0 39 24416.0 40 24009.0 41 22993.5 42 21840.5 43 22268.5 44 24084.0 45 25023.5 46 24313.5 47 23330.0 48 22734.0 49 21612.5 50 20945.5 51 19614.0 52 18041.5 53 17688.0 54 17441.5 55 16579.0 56 15385.0 57 14183.5 58 13506.0 59 12481.0 60 10347.0 61 8628.0 62 7516.5 63 5578.5 64 3840.5 65 3045.0 66 2212.0 67 1533.0 68 1070.5 69 663.5 70 476.5 71 401.0 72 400.5 73 447.5 74 324.5 75 191.0 76 116.5 77 63.0 78 43.0 79 17.5 80 9.0 81 5.0 82 2.5 83 3.0 84 2.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02725085036627938 2 0.002235967209540872 3 0.0020962192589445675 4 0.08496675396255314 5 0.001956471308348263 6 0.023757151601371766 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001117983604770436 26-27 0.0 28-29 4.891178270870658E-4 30-31 0.007057271505113377 32-33 0.0 34-35 0.0 36-37 6.987397529815225E-4 38-39 6.987397529815224E-5 40-41 1.397479505963045E-4 42-43 0.0 44-45 0.003982816591994679 46-47 0.0027250850366279376 48-49 0.0 50-51 0.0025853370860316337 52-53 6.987397529815224E-5 54-55 0.0020263452836464155 56-57 1.397479505963045E-4 58-59 0.003004580937820547 60-61 1.397479505963045E-4 62-63 0.0037731946661002215 64-65 0.0025853370860316337 66-67 2.0962192589445676E-4 68-69 9.782356541741316E-4 70-71 0.0036334467155039168 72-73 0.0032840768390131557 74-75 0.0 76-77 0.0020263452836464155 78-79 0.001117983604770436 80-81 2.79495901192609E-4 82-83 0.0011878575800685883 84-85 0.0 86-87 0.004122564542590983 88-89 0.005380296097957723 90-91 0.005450170073255875 92-93 0.006218783801535551 94-95 0.003912942616696526 96-97 0.004821304295572506 98-99 0.005310422122659571 100-101 0.0064982797027281596 102-103 0.0026552110613297856 104-105 0.0020263452836464155 106-107 0.0 108-109 0.008175255109883813 110-111 0.002445589135435329 112-113 0.005030926221466963 114-115 0.0 116-117 2.0962192589445676E-4 118-119 0.0012577315553667407 120-121 2.79495901192609E-4 122-123 0.0025154631107334813 124-125 0.04241350300597842 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 715574.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.45845845517767 #Duplication Level Percentage of deduplicated Percentage of total 1 65.78145320976046 25.298532853887863 2 14.956585462728697 11.504144412993316 3 6.640115494538493 7.661058176528704 4 3.5899484227085727 5.52255529083873 5 2.1678380838121725 4.16858554419212 6 1.45963320076955 3.3681145686956246 7 1.0115467141343073 2.7231769096704017 8 0.7209516002295296 2.2181349732496978 9 0.563237975882539 1.9495137866271286 >10 2.8447765169645445 19.234822837800706 >50 0.1708529816187399 4.500119543542759 >100 0.0872440657990592 6.121135062802073 >500 0.0029081355266351526 0.7106187083003381 >1k 0.0018175847041469705 1.3630793637474519 >5k 7.270338816587882E-4 2.0632128461728096 >10k+ 3.635169408293941E-4 1.593195120950355 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11396 1.5925676449954862 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8432 1.1783547194280395 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6326 0.8840455354722223 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2666 0.3725680362897478 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2257 0.3154111244958593 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1847 0.2581144647513744 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1668 0.23309958159463592 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1312 0.1833493111823515 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 839 0.11724853055029949 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 754 0.10536995474961361 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 716 0.10005953262695402 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.987397529815226E-5 0.0 18-19 0.0 0.0 0.0 2.7949590119260903E-4 0.0 20-21 0.0 0.0 0.0 5.589918023852181E-4 0.0 22-23 0.0 0.0 0.0 0.001397479505963045 0.0 24-25 0.0 0.0 0.0 0.0025853370860316333 0.0 26-27 0.0 0.0 0.0 0.004611682369678049 0.0 28-29 0.0 0.0 0.0 0.0071970194557096825 0.0 30-31 0.0 0.0 0.0 0.016630006120960238 0.0 32-33 0.0 0.0 0.0 0.03360938211841123 0.0 34-35 0.0 0.0 0.0 0.052894599300701256 0.0 36-37 0.0 0.0 0.0 0.0791672140128065 0.0 38-39 0.0 0.0 0.0 0.1312931995852281 0.0 40-41 0.0 0.0 0.0 0.19781322406906904 0.0 42-43 0.0 0.0 0.0 0.25986131413382824 0.0 44-45 0.0 0.0 0.0 0.32407549743283015 0.0 46-47 0.0 0.0 0.0 0.3996791387054309 0.0 48-49 0.0 0.0 0.0 0.4879299695069972 0.0 50-51 0.0 0.0 0.0 0.5940685379848905 0.0 52-53 0.0 0.0 0.0 0.6997878626109948 0.0 54-55 0.0 0.0 0.0 0.8018737405215952 0.0 56-57 0.0 0.0 0.0 0.9241531972933617 0.0 58-59 0.0 0.0 0.0 1.059289465519988 0.0 60-61 0.0 0.0 0.0 1.2336949078641761 0.0 62-63 0.0 0.0 0.0 1.40977732561552 0.0 64-65 0.0 0.0 0.0 1.5727234360108109 0.0 66-67 0.0 0.0 0.0 1.759147202106281 0.0 68-69 0.0 0.0 0.0 1.9665331607911969 0.0 70-71 0.0 0.0 0.0 2.2196166993211044 0.0 72-73 0.0 0.0 0.0 2.4792683915290383 0.0 74-75 0.0 0.0 0.0 2.720123984381769 0.0 76-77 0.0 0.0 0.0 2.988999041329059 0.0 78-79 0.0 0.0 0.0 3.2926014639995307 0.0 80-81 0.0 0.0 0.0 3.6419014665149936 0.0 82-83 0.0 0.0 0.0 4.01943055505091 0.0 84-85 0.0 0.0 0.0 4.385360563687334 0.0 86-87 0.0 0.0 0.0 4.754504775187472 0.0 88-89 0.0 0.0 0.0 5.175774972260031 0.0 90-91 0.0 0.0 0.0 5.651896239941641 0.0 92-93 0.0 0.0 0.0 6.172177860011683 0.0 94-95 0.0 0.0 0.0 6.697769902204384 0.0 96-97 0.0 0.0 0.0 7.19974454074631 0.0 98-99 0.0 0.0 0.0 7.735608057307839 0.0 100-101 0.0 0.0 0.0 8.353992738696487 0.0 102-103 0.0 0.0 0.0 9.030582441508496 0.0 104-105 0.0 0.0 0.0 9.71520765147979 0.0 106-107 0.0 0.0 0.0 10.377682811281574 0.0 108-109 0.0 0.0 0.0 11.05622898540193 0.0 110-111 0.0 0.0 0.0 11.808631951412433 0.0 112-113 0.0 0.0 0.0 12.636568684720238 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2830 0.0 90.56491 1 GTATCAA 6440 0.0 64.23201 1 TATAGCG 30 0.004233496 59.50853 5 ATCAACG 8710 0.0 47.26672 3 TCAACGC 8745 0.0 47.07754 4 TATCAAC 8815 0.0 46.774563 2 TGGTATC 920 0.0 45.98291 2 CAACGCA 8965 0.0 45.867702 5 AACGCAG 9185 0.0 44.89865 6 GTGGTAT 1060 0.0 39.923668 1 ACGCAGA 10400 0.0 39.642197 7 CGCAGAG 10480 0.0 39.282818 8 GTACATG 6680 0.0 38.189613 1 GCAGAGT 11150 0.0 36.7089 9 TACATGG 6960 0.0 36.469208 2 ACATGGG 6945 0.0 35.087032 3 CGTATAG 120 9.155556E-7 34.769203 1 CATGGGA 5085 0.0 33.39254 4 CGCGATA 45 2.3942163E-4 33.053364 104-105 CATATAG 260 0.0 32.081173 3 >>END_MODULE