FastQCFastQC Report
Fri 27 May 2016
SRR940164_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940164_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2609024
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG51000.19547539616346954No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA45900.1759278565471226No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA43830.16799385517342885No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA39170.15013276995535493No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA39090.1498261418829417No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA33210.12728897856056517No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA32480.12449099739979394No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC31290.11992990482264632No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG31240.11973826227738803No Hit
CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA27660.10601665603689348No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT26840.10287371829465732No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23100.060.5518571
GTATCAA47000.057.7482151
ATCAACG52750.050.098393
TCAACGC52750.049.9855584
CAACGCA56400.046.9617355
AACGCAG57050.046.3134736
TATCAAC60900.044.0780642
GTACATG130100.038.7505341
ACGCAGA69850.037.563757
TACATGG133200.036.9989472
ACATGGG128500.036.3604973
CGCAGAG73150.035.872588
TAAGGTG16150.034.27475
CATGGGG71500.032.2157484
AGGTAAG14700.031.1771682
GTAAGGT16750.030.9148884
CATGGGA64200.027.6276864
GCAGAGT92900.026.9652679
GGTAAGG18700.026.417993
GTGTAAG12850.025.4759961