FastQCFastQC Report
Fri 27 May 2016
SRR940164_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940164_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2609024
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53210.20394599666388658No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG46340.17761431094539568No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA45620.1748546582936761No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA45040.17263160476867978No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43180.1655025020850709No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA37780.14480510719717413No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA34470.13211837070107443No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA34380.1317734141196095No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC33260.12748062110582348No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA31280.11989157631359466No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG29860.11444892802825885No Hit
CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA29190.11188091792179759No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG26210.10045902222440269No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42600.077.892651
GTATCAA87750.061.508051
ATCAACG111350.047.812323
TCAACGC113650.046.791424
CAACGCA115850.045.9163785
TATCAAC116850.045.665532
AACGCAG119050.044.730446
ACGCAGA139000.038.1776857
GTGGTAT17200.037.752781
CGCAGAG143150.036.9877748
GTACATG132900.035.9949381
TACATGG131550.035.3112372
ACATGGG127100.035.281093
TGGTATC18800.032.9446332
GCAGAGT166000.031.5021619
CATGGGA61400.029.8721564
CATGGGG69200.029.0867254
GAGTACT96650.027.17617812-13
AGAGTAC148750.026.9764310-11
TAAGGTG17050.026.8715365
Produced by FastQC (version 0.11.5)