FastQCFastQC Report
Fri 27 May 2016
SRR940163_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940163_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047994
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG31480.15371138782633154No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28350.13842813992619118No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27060.13212929334753912No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24280.11855503482920361No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23760.11601596489052214No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA21710.106006169939951No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC20580.10048857565012398No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21450.068.807581
GTATCAA41300.054.037231
TCAACGC49700.043.34754
ATCAACG49900.043.1737633
CAACGCA52600.041.070765
AACGCAG54050.039.9640776
GTACATG115800.039.2641941
TATCAAC57600.037.7122342
TACATGG117950.037.5393642
ACATGGG116400.036.50543
ACGCAGA65650.032.7173657
CGCAGAG66800.031.8001658
TAAGGTG10700.031.703195
CATGGGA72500.031.4392854
TATGCCG1152.7608909E-531.0502645
GTAAGGT10000.030.9467624
CATGGGG52100.030.6132164
GTATAGG7650.028.7840651
GTATAGA5950.027.0059031
GCAGAGT77950.026.4881119
Produced by FastQC (version 0.11.5)