FastQCFastQC Report
Fri 27 May 2016
SRR940163_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940163_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047994
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67720.33066503124520874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54160.26445389976728445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32620.15927781038421013No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG29070.14194377522590398No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26750.13061561703794053No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25600.12500036621201038No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22180.10830109853837463No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC21320.1041018674859399No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA20960.1023440498360835No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA20480.10000029296960831No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38950.081.801431
GTATCAA87650.064.616461
GCGCTAG300.004221690459.55451
ATCAACG112800.049.8362853
TCAACGC113000.049.748084
CAACGCA116650.048.201275
TATCAAC117750.047.8929752
AACGCAG119000.047.348246
ACGCAGA137550.040.82917
CGCAGAG139600.040.229538
TACCGTA650.001940536536.610457
GCAGAGT152400.036.5383839
GTACATG114900.035.6601371
TACATGG118900.034.207522
ACATGGG117450.033.767893
GTGGTAT15450.031.9936811
CCGTTGC2500.030.935839
GAGTACT90200.030.14172412-13
TAAGGTG12900.029.5190035
CATGGGA69650.029.3261994
Produced by FastQC (version 0.11.5)