FastQCFastQC Report
Fri 27 May 2016
SRR940162_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940162_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676500
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43550.056.850041
GGTATCA27100.052.4873051
ATCAACG50350.046.0989233
TCAACGC50900.045.6007964
CAACGCA52700.044.0432785
TATCAAC54850.043.8346332
AACGCAG54450.042.4028136
ACCGTAC600.001308801239.6659438
GTACATG109700.037.2714041
TACATGG112200.035.644222
ACGCAGA65750.034.8396647
ACATGGG112650.034.0764353
CGCAGAG67050.033.8979648
CATGGGA74550.030.336234
CATGGGG43300.029.688724
GCAGAGT77050.028.417399
GTGGTAT12300.027.0962091
ATGGGAG34150.026.6640645
GTAAGGT8000.026.0377644
ATGGGAT31050.024.725995
Produced by FastQC (version 0.11.5)