FastQCFastQC Report
Fri 27 May 2016
SRR940162_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940162_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676500
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73640.4392484342379958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59950.35759021771547866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34430.20536832687145842No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44750.073.0824661
GTATCAA83000.063.733651
ATCAACG110800.047.5177233
TCAACGC111300.047.197234
TATCAAC112200.047.0309752
CAACGCA113050.046.42925
AACGCAG116050.045.227616
ACGCAGA134300.039.1154487
CGCAGAG136400.038.295158
GTGGTAT14650.035.7831841
GTACATG106900.034.8286171
GCAGAGT148200.034.6849
TGGTATC14450.034.2100222
TACATGG109700.033.1724622
ACATGGG106900.032.2027743
GAGTACT92250.029.14980912-13
AGAGTAC141950.028.47853910-11
CATGGGA68850.028.4600144
CATGGGG42350.028.2673954
CAGAGTA146350.028.25242410-11
Produced by FastQC (version 0.11.5)