FastQCFastQC Report
Fri 27 May 2016
SRR940161_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940161_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479599
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG22700.15341994689101573No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA22530.15227098693632532No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC19140.12935937372220446No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA18460.12476353390344277No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA18320.12381733158781534No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA16030.10834016513933842No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA15740.10638017462839594No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA15720.1062450028690206No Hit
CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA15480.10462294175651646No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22250.073.833811
GTATCAA41800.062.7969471
ATCAACG52850.048.315733
TCAACGC53050.048.1335754
TATCAAC55250.047.186532
CAACGCA55050.046.4929775
AACGCAG56500.045.598886
ACGCAGA68500.037.2620547
CGCAGAG68750.036.6950848
GTACATG116750.036.401361
TACATGG118750.035.086552
ACATGGG119700.033.4157643
GCAGAGT78500.031.3794579
CATGGGA75750.031.0377834
ATGGGAG30900.029.6645975
CATGGGG45800.028.461314
CAGAGTA74800.026.05100310-11
GTAAGGT9700.025.7723374
CGATACC3250.025.651445118-119
AGAGTAC69850.025.1713110-11
Produced by FastQC (version 0.11.5)