FastQCFastQC Report
Fri 27 May 2016
SRR940161_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940161_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479599
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64950.43897028857143056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51460.3477969368727608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29670.2005273050333232No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG21870.14781031887693896No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20970.14172758970504845No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC19710.13321176886440178No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA18110.12239802811437424No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA17310.11699115773936046No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA16070.1086105086580891No Hit
CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA15100.10205467832838493No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36000.087.400431
GTATCAA80850.065.377121
TATCAAC108050.049.01312
ATCAACG108000.048.9238243
TCAACGC108200.048.7783364
CAACGCA110450.047.837495
AACGCAG113450.046.67746
TGGTATC10150.046.365642
ACGCAGA130400.040.4690637
CGCAGAG132050.039.8282248
GTACATG113500.036.489771
GCAGAGT145550.035.8479659
TACATGG115400.035.412252
ACATGGG116850.033.6971553
GTGGTAT14950.033.0840841
GAGTACT83800.030.84770612-13
CATGGGA74050.030.4884014
AGTACTT85200.029.08389312-13
CATGGGG46200.029.0108914
AGAGTAC137200.028.94504710-11
Produced by FastQC (version 0.11.5)