FastQCFastQC Report
Fri 27 May 2016
SRR940159_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940159_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480943
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57341.1922410763853513No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22320.4640882599393275No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19510.4056613777516254No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10330.21478636761528913No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7050.14658701758836287No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5590.11622998983247496No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5470.11373489166075813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5440.11311111711782894No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9900.070.356421
GTATCAA19750.064.505941
ATCAACG26200.046.3486143
GAACAAA11700.046.3029441
TCAACGC26200.046.1214144
TATCAAC27850.045.7400322
GTGGTAC1352.7284841E-1144.0751156
CAACGCA27650.043.7027555
AACGCAG27400.043.648846
ACGCAAA1800.042.995591
GTACATG47050.038.7181741
CGCAGAG31650.037.2197578
ACGCAGA31850.037.1728367
TACATGG47600.036.8912162
GGTGGTA1652.3464963E-1036.076465
GTCGGGA2350.035.4623952
GTATGGT1703.237801E-1035.019031
CTAGACT1051.4774423E-534.0149544
ATGGGAG13150.033.9502835
CATGGGA35550.033.6561434
Produced by FastQC (version 0.11.5)