##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940159_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 480943 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.079019759098273 33.0 31.0 34.0 23.0 34.0 2 30.225028745610185 33.0 31.0 34.0 23.0 34.0 3 30.43999392859445 34.0 31.0 34.0 24.0 34.0 4 33.196474426283366 37.0 35.0 37.0 22.0 37.0 5 33.76803280222396 37.0 35.0 37.0 28.0 37.0 6 33.36405769498673 37.0 35.0 37.0 22.0 37.0 7 33.33362581428568 37.0 35.0 37.0 23.0 37.0 8 33.09373667981445 37.0 35.0 37.0 18.0 37.0 9 34.3259845761348 39.0 37.0 39.0 17.0 39.0 10-11 34.56841663149271 39.0 37.0 39.0 19.5 39.0 12-13 34.47572893253462 39.0 37.0 39.0 17.5 39.0 14-15 35.81082685474162 41.0 37.0 41.0 17.5 41.0 16-17 35.970752251306294 41.0 37.0 41.0 23.0 41.0 18-19 36.01862382860339 41.0 37.0 41.0 24.5 41.0 20-21 36.092071201784826 41.0 37.0 41.0 25.0 41.0 22-23 36.14740312261536 40.0 36.5 41.0 26.0 41.0 24-25 36.217193097726756 40.5 36.0 41.0 27.0 41.0 26-27 36.20878773576079 40.0 36.0 41.0 27.0 41.0 28-29 36.1121390684551 40.0 36.0 41.0 27.0 41.0 30-31 35.97827393266978 40.0 36.0 41.0 27.0 41.0 32-33 35.867503009712166 40.0 35.5 41.0 26.0 41.0 34-35 35.73625980625563 40.0 35.0 41.0 25.0 41.0 36-37 35.54433061714174 40.0 35.0 41.0 25.0 41.0 38-39 35.35655056836257 40.0 35.0 41.0 25.0 41.0 40-41 35.213523224165854 40.0 35.0 41.0 25.0 41.0 42-43 35.12660336048138 40.0 35.0 41.0 25.0 41.0 44-45 35.173811657514506 40.0 35.0 41.0 25.0 41.0 46-47 35.05128466367117 40.0 35.0 41.0 25.0 41.0 48-49 34.910207654545346 40.0 35.0 41.0 24.0 41.0 50-51 34.7826998209767 40.0 35.0 41.0 24.0 41.0 52-53 34.597334819302915 40.0 34.0 41.0 23.0 41.0 54-55 34.42022027558359 39.0 34.0 41.0 22.0 41.0 56-57 34.22321668056298 39.0 34.0 41.0 20.5 41.0 58-59 33.97521223928823 39.0 33.5 41.0 18.0 41.0 60-61 33.74120529875682 38.0 33.0 41.0 14.0 41.0 62-63 33.45711030205243 37.5 33.0 41.0 7.5 41.0 64-65 33.15683251445597 37.0 33.0 40.0 4.0 41.0 66-67 32.83878546937995 36.5 33.0 40.0 2.0 41.0 68-69 32.52114179850835 36.0 32.5 39.5 2.0 41.0 70-71 32.393719214127245 36.0 33.0 39.0 2.0 41.0 72-73 32.151068837679304 35.0 33.0 39.0 2.0 41.0 74-75 31.82703875511235 35.0 33.0 38.0 2.0 40.5 76-77 31.483856714828992 35.0 33.0 37.0 2.0 39.5 78-79 31.161893405247607 35.0 32.0 37.0 2.0 39.0 80-81 30.85113412608147 35.0 32.0 36.0 2.0 39.0 82-83 30.564697895592616 35.0 32.0 36.0 2.0 37.0 84-85 30.31477846647108 35.0 32.0 35.5 2.0 37.0 86-87 30.099283075125328 35.0 32.0 35.0 2.0 37.0 88-89 29.90651906774815 35.0 31.0 35.0 2.0 36.0 90-91 29.754265058437277 35.0 31.0 35.0 2.0 36.0 92-93 29.607062583299893 35.0 31.0 35.0 2.0 36.0 94-95 29.487555697868565 35.0 31.0 35.0 2.0 36.0 96-97 29.35874105663249 35.0 31.0 35.0 2.0 35.0 98-99 29.266027367068446 35.0 31.0 35.0 2.0 35.0 100-101 29.18091748918271 35.0 30.5 35.0 2.0 35.0 102-103 28.461800046991016 34.0 29.0 35.0 2.0 35.0 104-105 28.963343888984767 35.0 30.0 35.0 2.0 35.0 106-107 29.00962380157316 35.0 30.0 35.0 2.0 35.0 108-109 28.944615058333316 35.0 30.0 35.0 2.0 35.0 110-111 28.837253063252817 35.0 30.0 35.0 2.0 35.0 112-113 28.718832585150423 35.0 30.0 35.0 2.0 35.0 114-115 28.579417935181507 35.0 29.0 35.0 2.0 35.0 116-117 28.47941751933181 35.0 29.0 35.0 2.0 35.0 118-119 28.337901788777465 35.0 29.0 35.0 2.0 35.0 120-121 28.167433562813056 35.0 29.0 35.0 2.0 35.0 122-123 27.933606477274854 35.0 29.0 35.0 2.0 35.0 124-125 26.101976741526542 33.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7454934745011741 1101 2 0.8638510489399849 1101 3 0.6010542063249815 1101 4 0.9716647581377842 1101 5 0.723776329895486 1101 6 0.7751816205719138 1101 7 0.9055296611214843 1101 8 0.755141654656704 1101 9 1.123413423211126 1101 10-11 1.0273421931448325 1101 12-13 1.2088055118204863 1101 14-15 1.094779264869345 1101 16-17 0.9563193867610948 1101 18-19 0.9903645472096017 1101 20-21 0.9119908036225794 1101 22-23 0.7116205065496786 1101 24-25 0.8792870017664995 1101 26-27 0.8149155743537193 1101 28-29 0.7443647346652895 1101 30-31 0.6864183476653452 1101 32-33 0.6236108058823859 1101 34-35 0.7073733044809813 1101 36-37 0.717697312068033 1101 38-39 0.7188097371371001 1101 40-41 0.7573811253795952 1101 42-43 0.8512139191243975 1101 44-45 0.672599080150917 1101 46-47 0.7971672354648973 1101 48-49 0.7517101090565319 1101 50-51 0.6179666957153245 1101 52-53 0.7614257843501875 1101 54-55 0.828497892731491 1101 56-57 0.7588135797825117 1101 58-59 0.962927986071783 1101 60-61 0.8359218993477242 1101 62-63 0.8018584396173125 1101 64-65 0.7994519907456876 1101 66-67 0.7733420699844871 1101 68-69 0.8475143826268337 1101 70-71 0.8164911562949442 1101 72-73 0.7900781165269848 1101 74-75 0.6749236578452518 1101 76-77 0.6828421031059193 1101 78-79 0.6837441841259881 1101 80-81 0.6943739568647018 1101 82-83 0.7003375218416004 1101 84-85 0.675708755368408 1101 86-87 0.6022088569155741 1101 88-89 0.7371008008893298 1101 90-91 0.7108949484643325 1101 92-93 0.7692777157616355 1101 94-95 0.7757021972581306 1101 96-97 0.7432550964071183 1101 98-99 0.8203106892313272 1101 100-101 0.6429853341965277 1101 102-103 0.7724824324555222 1101 104-105 0.6605793831720668 1101 106-107 0.7266737272890538 1101 108-109 0.6178989438433788 1101 110-111 0.6600684448432048 1101 112-113 0.7690425151936466 1101 114-115 0.8041317824870013 1101 116-117 0.8091600212376413 1101 118-119 0.9654409309630232 1101 120-121 0.910592563324446 1101 122-123 0.9652541544150672 1101 124-125 1.0658140650961307 1102 1 -0.017282887355378307 1102 2 0.08890903311890241 1102 3 0.007915740987613162 1102 4 0.15123051616517103 1102 5 -0.18704929426120742 1102 6 0.13618715001376813 1102 7 -0.033961753692480556 1102 8 -0.08241225078719339 1102 9 -0.10246030797157601 1102 10-11 0.04311146461661508 1102 12-13 0.10375383314543285 1102 14-15 -0.0799416173720644 1102 16-17 0.02277851563147948 1102 18-19 0.062105086749078 1102 20-21 -0.07069992684578352 1102 22-23 -0.10877845987682377 1102 24-25 -0.06299448827256526 1102 26-27 -0.17533294186862491 1102 28-29 -0.09980443803496541 1102 30-31 -0.06839345729616042 1102 32-33 -0.1868525825101912 1102 34-35 -0.02809757996514861 1102 36-37 -0.06709760472576676 1102 38-39 -0.29723071509540233 1102 40-41 -0.17289345065243822 1102 42-43 -0.24571225797648566 1102 44-45 -0.3292396576900529 1102 46-47 -0.10241210364774389 1102 48-49 -0.13595270963597983 1102 50-51 -0.33845036706632925 1102 52-53 -0.19773946394555963 1102 54-55 -0.06658381425219062 1102 56-57 -0.05097406142990479 1102 58-59 -0.1846593870464872 1102 60-61 -0.08851677852481288 1102 62-63 0.02153028884962538 1102 64-65 -0.19970249850850053 1102 66-67 -0.12241758890098708 1102 68-69 -0.06317124170624311 1102 70-71 -0.048950273070175854 1102 72-73 -0.19958504610210426 1102 74-75 -0.1941103654189682 1102 76-77 -0.0334598291121182 1102 78-79 -0.023276972382792138 1102 80-81 0.04741933885477323 1102 82-83 -0.19253505406696902 1102 84-85 -0.1712824610206134 1102 86-87 -0.3109273155112966 1102 88-89 -0.0854213124686396 1102 90-91 -0.2080065710298129 1102 92-93 -0.18363604434701486 1102 94-95 -0.21963591885133127 1102 96-97 -0.18742625076598785 1102 98-99 -0.20805415692319684 1102 100-101 -0.2256507368131473 1102 102-103 -0.18998218176228576 1102 104-105 -0.23819853962740112 1102 106-107 -0.2005539234477638 1102 108-109 -0.1333456215177975 1102 110-111 -0.19612307491551206 1102 112-113 -0.026005459549061527 1102 114-115 -0.04098321579331454 1102 116-117 -0.15505955139911975 1102 118-119 -0.08942577156711806 1102 120-121 -0.09963844740813599 1102 122-123 -0.18746029885806337 1102 124-125 -0.06210167926198551 1103 1 -0.30412352821569755 1103 2 -0.21054946717497813 1103 3 -0.20379209500256223 1103 4 -0.3095224880072607 1103 5 -0.31953234865030566 1103 6 -0.09502182685795901 1103 7 -0.7281809146919898 1103 8 -0.34055541857164684 1103 9 -0.29329034679059873 1103 10-11 -0.23736549315176347 1103 12-13 -0.3429297884439322 1103 14-15 -0.2902091043709305 1103 16-17 -0.5459749623252605 1103 18-19 -0.5191411000694401 1103 20-21 -0.5004491116909477 1103 22-23 -0.584564059068704 1103 24-25 -0.3186324097897426 1103 26-27 -0.25821055578357743 1103 28-29 -0.3794153985432587 1103 30-31 -0.12215456720294782 1103 32-33 -0.2007454133937756 1103 34-35 -0.14254219078038943 1103 36-37 -0.24093398376215447 1103 38-39 -0.22179956020146108 1103 40-41 -0.3387827683806819 1103 42-43 -0.3251806362262073 1103 44-45 -0.4844934116484936 1103 46-47 -0.5184170739133123 1103 48-49 -0.24021877975158645 1103 50-51 -0.4449847912954752 1103 52-53 -0.5959395305435393 1103 54-55 -0.49901703189573965 1103 56-57 -0.41147028344702363 1103 58-59 -0.34623101004827106 1103 60-61 -0.5791360727202814 1103 62-63 -0.4451293753445711 1103 64-65 -0.5132348510487219 1103 66-67 -0.7419721422587031 1103 68-69 -0.6448018195666592 1103 70-71 -0.643060150802544 1103 72-73 -0.7507783344557275 1103 74-75 -0.6201284988428881 1103 76-77 -0.6737145105256914 1103 78-79 -0.4159100733681278 1103 80-81 -0.42149862271059035 1103 82-83 -0.2364338519510305 1103 84-85 -0.39851343757499436 1103 86-87 -0.18601363588335218 1103 88-89 -0.3036727354976847 1103 90-91 -0.2429045844879738 1103 92-93 -0.2691060629631643 1103 94-95 -0.3392776306429717 1103 96-97 -0.60397987219892 1103 98-99 -0.6151510882758728 1103 100-101 -0.5928078745301768 1103 102-103 -0.48174280447577544 1103 104-105 -0.3941272127408091 1103 106-107 -0.5714655783182394 1103 108-109 -0.4475093139960258 1103 110-111 -0.4426786406519696 1103 112-113 -0.44070528813747245 1103 114-115 -0.42507255542291134 1103 116-117 -0.46620957341221114 1103 118-119 -0.4965137635932386 1103 120-121 -0.5009784524588561 1103 122-123 -0.38931364473624797 1103 124-125 -0.22109542396822945 1104 1 -0.3460626085636953 1104 2 -0.3422940379354422 1104 3 -0.28449288131791306 1104 4 -0.398776178171417 1104 5 -0.2784390604861926 1104 6 -0.2583682692163194 1104 7 -0.43395604704489443 1104 8 -0.578159700903413 1104 9 -0.29903675839288013 1104 10-11 -0.2846586189418687 1104 12-13 -0.5462399114908649 1104 14-15 -0.38698559587117387 1104 16-17 -0.35788231415897087 1104 18-19 -0.44715943612031595 1104 20-21 -0.42715579141387394 1104 22-23 -0.3116355149393897 1104 24-25 -0.3716176717478703 1104 26-27 -0.4311400045034759 1104 28-29 -0.24094519688740235 1104 30-31 -0.21082479644881147 1104 32-33 -0.15818216555179276 1104 34-35 -0.3276021990573952 1104 36-37 -0.28814569640086773 1104 38-39 -0.5611895043203745 1104 40-41 -0.39842016325030016 1104 42-43 -0.19436779375386948 1104 44-45 -0.34328569277715104 1104 46-47 -0.5495937879428325 1104 48-49 -0.43175678725516775 1104 50-51 -0.38104719899897077 1104 52-53 -0.4899384766955279 1104 54-55 -0.5923504255351091 1104 56-57 -0.6064536502878966 1104 58-59 -0.6010383435640279 1104 60-61 -0.48721610184809805 1104 62-63 -0.6018847446549742 1104 64-65 -0.6505924063022448 1104 66-67 -0.3801144298325738 1104 68-69 -0.3383095730385932 1104 70-71 -0.4222846453060214 1104 72-73 -0.3796067313629301 1104 74-75 -0.3564030330352814 1104 76-77 -0.47961651170618325 1104 78-79 -0.4579984111603359 1104 80-81 -0.4020691479837204 1104 82-83 -0.43288039404049883 1104 84-85 -0.3809666419740161 1104 86-87 -0.31088671051885797 1104 88-89 -0.28382827984636805 1104 90-91 -0.24660435992336005 1104 92-93 -0.45014855836670975 1104 94-95 -0.4484549281334118 1104 96-97 -0.29011408177898446 1104 98-99 -0.24577509746651316 1104 100-101 -0.26318072414877847 1104 102-103 -0.4038541640980178 1104 104-105 -0.3520626204510897 1104 106-107 -0.36549030530149906 1104 108-109 -0.30355955495634035 1104 110-111 -0.11280272786354573 1104 112-113 -0.09026011650271926 1104 114-115 -0.05029163595182595 1104 116-117 -0.2601551487205036 1104 118-119 -0.20747106917976055 1104 120-121 -0.1494048040867817 1104 122-123 -0.3444029843625174 1104 124-125 -0.53499400579636 1105 1 -0.03353082983133859 1105 2 -0.0012863233742095304 1105 3 -0.022193416077271877 1105 4 0.043336121293819474 1105 5 -0.03643853449338508 1105 6 0.2868758791136514 1105 7 0.02585371888829968 1105 8 0.10869924535765563 1105 9 -0.04255170535527242 1105 10-11 0.13629781132024732 1105 12-13 0.13962622333862384 1105 14-15 -0.13103968546528222 1105 16-17 0.09261737901188383 1105 18-19 0.1906375306544703 1105 20-21 0.03218277262575242 1105 22-23 0.23159232761343418 1105 24-25 0.1543926727774192 1105 26-27 0.3047491425115183 1105 28-29 0.21411200483083803 1105 30-31 0.08906452589711478 1105 32-33 0.3172397245157086 1105 34-35 0.17373469933832553 1105 36-37 0.06595814334665562 1105 38-39 0.15701861350201085 1105 40-41 0.21448309790513065 1105 42-43 0.17497668770488417 1105 44-45 0.07093035877014131 1105 46-47 0.1799516666026193 1105 48-49 0.0782907712615355 1105 50-51 0.08582949951948393 1105 52-53 0.08251155398738774 1105 54-55 0.017384824749804295 1105 56-57 0.05237645403401103 1105 58-59 -0.026315585608386982 1105 60-61 0.1906242593336387 1105 62-63 0.2582603416955038 1105 64-65 0.03707087063297365 1105 66-67 -0.33088917341108015 1105 68-69 -0.16167899532315033 1105 70-71 -0.025970978259472588 1105 72-73 0.028410275737968504 1105 74-75 -0.13107757498320538 1105 76-77 -0.21647984124067676 1105 78-79 -0.05145747138651657 1105 80-81 0.043854407727685896 1105 82-83 0.14143033621285284 1105 84-85 0.18656645174037223 1105 86-87 0.10331399957143717 1105 88-89 -0.036683982485101296 1105 90-91 -0.1426758511129833 1105 92-93 -0.06813422506260025 1105 94-95 -0.09729445649319146 1105 96-97 0.02698616368432738 1105 98-99 0.016819142912204654 1105 100-101 0.1449710686100545 1105 102-103 0.06617739547067103 1105 104-105 -0.01652523112874249 1105 106-107 -0.07313254252425949 1105 108-109 0.10217157505155328 1105 110-111 0.05141222786539146 1105 112-113 -0.030763754619666628 1105 114-115 -0.13303359264508785 1105 116-117 -0.015901620185392318 1105 118-119 0.03515297745827084 1105 120-121 0.0642206013660136 1105 122-123 0.16140156547530182 1105 124-125 0.18556573822047184 1106 1 0.00501726296560534 1106 2 0.03819364882146559 1106 3 0.018179335682219744 1106 4 0.07107745416374911 1106 5 0.25780944534847805 1106 6 0.08458202213216026 1106 7 0.12800420980845217 1106 8 0.36528105090114593 1106 9 0.21589168677262904 1106 10-11 0.32489369280430225 1106 12-13 0.26728935653872554 1106 14-15 0.18666602311061098 1106 16-17 -0.010428063685139932 1106 18-19 0.12517137373379938 1106 20-21 -0.01297536448433334 1106 22-23 0.033165009862109684 1106 24-25 0.006212987313872986 1106 26-27 0.05301028596446855 1106 28-29 0.09749783331289308 1106 30-31 0.24971162816490988 1106 32-33 0.08926922316893382 1106 34-35 0.10485382350202599 1106 36-37 0.11317445232223378 1106 38-39 0.1351487725080105 1106 40-41 0.09471052861153595 1106 42-43 0.17210610815843808 1106 44-45 0.09434790156274175 1106 46-47 0.23213039629242616 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-0.3339922567037519 2216 50-51 -0.23688425811472058 2216 52-53 -0.2997248651704396 2216 54-55 -0.2624448120229417 2216 56-57 -0.23273283558347657 2216 58-59 -0.2125137534000885 2216 60-61 -0.2190591222157039 2216 62-63 -0.3889190218531908 2216 64-65 -0.4009153074448193 2216 66-67 -0.17271254210281484 2216 68-69 -0.16101200721643494 2216 70-71 -0.25964182364680966 2216 72-73 -0.23544379047867992 2216 74-75 -0.027987732488831796 2216 76-77 -0.13788774866594622 2216 78-79 -0.26142613098150136 2216 80-81 -0.10132399150085902 2216 82-83 -0.0873264943882397 2216 84-85 -0.029650198854294985 2216 86-87 -0.11590409750345998 2216 88-89 0.09640023480837456 2216 90-91 -0.119182601354062 2216 92-93 -0.1828360894148986 2216 94-95 -0.06623932952436817 2216 96-97 -0.10829589996080102 2216 98-99 -0.09694250928966142 2216 100-101 -0.21648425122279846 2216 102-103 -0.19655163056597402 2216 104-105 -0.12767116491209052 2216 106-107 -0.17040833808838585 2216 108-109 -0.114438839606116 2216 110-111 -0.18031390521078805 2216 112-113 -0.03760239171384683 2216 114-115 -0.2614689831947494 2216 116-117 -0.2435133206032667 2216 118-119 -0.29445566927911315 2216 120-121 -0.20827216623283107 2216 122-123 -0.31549091282749586 2216 124-125 -0.9045419441967617 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13789.0 3 3156.0 4 3499.0 5 3058.0 6 4212.0 7 3327.0 8 2461.0 9 2259.0 10 2491.0 11 2809.0 12 2698.0 13 2482.0 14 2344.0 15 2173.0 16 1997.0 17 1904.0 18 1690.0 19 1584.0 20 1373.0 21 1435.0 22 1687.0 23 2932.0 24 5814.0 25 4934.0 26 7949.0 27 5668.0 28 5069.0 29 6401.0 30 6967.0 31 7480.0 32 9639.0 33 13206.0 34 18842.0 35 32056.0 36 64138.0 37 140876.0 38 86172.0 39 372.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.10925774945508 20.416881582669173 12.784313072993795 20.689547594881947 2 14.438679490512088 25.338809461918377 37.96932674811542 22.25318429945412 3 17.088635234080783 33.45547027227111 27.897130475107147 21.55876401854097 4 11.15107315064048 20.771986171146622 35.79255887871463 32.28438179949827 5 11.795747548662737 44.7853181850328 30.247026570540687 13.171907695763775 6 27.039792355159197 40.42793471998935 18.093635677310267 14.438637247541184 7 25.985630660137037 39.43479210206182 19.713029102592095 14.866548135209042 8 25.724414687902858 38.04071193911923 19.1770283195409 17.057845053437017 9 27.24070238481438 21.460210116537322 17.980102978730482 33.31898451991782 10-11 22.971792856519084 32.92496808346952 24.879819188176537 19.223419871834857 12-13 24.13943676761544 30.99015325817885 25.253758157533312 19.6166518166724 14-15 21.6958746914389 31.189470553860676 23.817485718326086 23.29716903637434 16-17 20.57124024818271 34.59223264633964 24.096137531812964 20.740389573664686 18-19 21.177627734721842 32.91501957791553 25.94723632197197 19.960116365390654 20-21 23.242844800608825 31.552742466884233 25.14750174922779 20.05691098327915 22-23 22.60270198620417 32.36831845802773 24.9161802086528 20.1127993471153 24-25 21.524068758077245 32.141063643745575 25.655978798241225 20.678888799935955 26-27 21.361686112939125 32.234340838419726 25.322986939132804 21.08098610950835 28-29 21.418664615000726 32.386621197313424 25.3203302073153 20.87438398037055 30-31 21.175450524910644 32.14316160677342 25.58349395661404 21.097893911701902 32-33 20.879220272904483 32.67170890188434 25.123430799220277 21.325640025990904 34-35 21.45156947362621 32.32336989010098 25.255566425438435 20.969494210834373 36-37 21.638641701522424 32.042448646889596 25.006732021862693 21.312177629725284 38-39 21.168484984582246 32.19264813209159 25.213800502766464 21.425066380559695 40-41 21.091420277076516 32.25256717920138 25.507197251693775 21.14881529202833 42-43 21.24438039866419 32.06572594228394 25.338631666063637 21.351261992988235 44-45 20.909534171209003 32.17365629909021 25.45886844159908 21.457941088101702 46-47 21.229223375872603 32.412677353817614 25.07605705266704 21.28204221764274 48-49 21.085670738997585 32.38038502604857 24.80368703455919 21.730257200394654 50-51 21.255285722752078 32.197810538486095 25.25969833300235 21.287205405759472 52-53 21.287917224629666 32.25033061906861 25.23340874497833 21.22834341132339 54-55 20.92993516674083 32.39521256908547 25.195248333423436 21.47960393075026 56-57 21.064862228978235 32.34720730481182 25.08471298440283 21.503217481807113 58-59 21.11202152836412 31.649835501004464 25.194237751999566 22.043905218631853 60-61 20.777946200406294 32.15011238571693 25.259443623124216 21.81249779075256 62-63 21.166182516099653 31.94343651943915 25.207366851662783 21.683014112798418 64-65 20.80655809287166 32.164077079423826 25.23191924022602 21.797445587478492 66-67 20.919541185876277 32.496561389209155 25.12987708330951 21.45402034160506 68-69 21.175110512286306 31.998349073277026 25.42645474922132 21.40008566521535 70-71 20.926134248814236 31.99310478194601 25.485690759035418 21.59507021020434 72-73 21.096476591179158 32.16063109677808 25.00621178711296 21.7366805249298 74-75 20.888463305947695 32.45364507630342 25.146233487000657 21.51165813074823 76-77 21.254601035622933 31.974026244099235 25.35051053299229 21.420862187285543 78-79 21.263914316814454 31.96277247733319 25.47581843265559 21.297494773196764 80-81 21.369451418887657 32.02324698885995 25.43954587277576 21.16775571947663 82-83 21.419541436820207 31.748441064006304 25.30269037316738 21.52932712600611 84-85 21.271335688428774 31.548541095306515 25.61935613991679 21.560767076347926 86-87 21.389963477279082 31.418376968957247 25.541057131599278 21.650602422164393 88-89 21.58836305262972 31.32893132175752 25.374675229483707 21.708030396129057 90-91 21.265661437436904 31.79010580794875 25.38766963633131 21.556563118283034 92-93 21.434394268661684 31.456229919000133 25.42241585476173 21.686959957576452 94-95 21.03794004694943 31.32803679438104 25.68370888026232 21.950314278407212 96-97 21.22218987815528 31.198694223811703 25.64924938661787 21.929866511415145 98-99 20.976424745492046 31.79093336216648 25.230283951028014 22.00235794131346 100-101 20.71399313432153 31.961791729997106 25.60604894966763 21.718166186013725 102-103 20.603710422763637 32.22433035134138 25.361555695327404 21.810403530567584 104-105 20.151572179258174 32.68273815588915 25.48144805601246 21.68424160884022 106-107 20.157170392333395 32.93706322786692 25.214942311251022 21.690824068548665 108-109 20.278078691237837 33.09550196177094 24.756987834317165 21.86943151267406 110-111 20.251985432709454 33.43199418639104 24.803691932785867 21.512328448113642 112-113 19.883636787077776 33.72900845899839 25.058119224467053 21.329235529456778 114-115 19.882955817848565 33.93875817557459 24.816943023873563 21.361342982703285 116-117 19.877469505126925 33.90765820791094 24.88577093056016 21.329101356401974 118-119 19.814706610135303 34.04981774250228 24.56099128116728 21.574484366195133 120-121 19.68966538963368 34.014024837165294 24.994359915372737 21.30194985782828 122-123 19.66677963916722 33.85151670780113 24.967199855284306 21.51450379774734 124-125 20.092078636195748 34.146711960338564 24.4368376970977 21.32437170636799 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4773.0 1 4427.5 2 5049.5 3 4849.5 4 2834.0 5 1591.0 6 1065.5 7 964.5 8 1042.0 9 1018.5 10 775.0 11 574.5 12 517.0 13 490.0 14 502.0 15 498.5 16 501.0 17 536.5 18 615.0 19 691.0 20 816.5 21 991.5 22 1153.5 23 1429.0 24 1805.0 25 2362.0 26 2879.0 27 3395.0 28 3947.0 29 4733.5 30 6026.5 31 7376.0 32 8430.0 33 9102.0 34 9955.0 35 10632.5 36 11247.0 37 12178.0 38 12989.5 39 13722.5 40 13889.5 41 14056.5 42 14812.0 43 15844.0 44 16760.0 45 17212.5 46 17151.0 47 16831.0 48 16189.0 49 15667.5 50 15288.5 51 14250.0 52 13372.5 53 13384.5 54 13155.0 55 12391.5 56 11396.0 57 10331.0 58 10085.5 59 9696.0 60 8778.0 61 7983.0 62 6929.0 63 5048.5 64 3361.5 65 2543.0 66 1954.0 67 1514.5 68 1112.0 69 807.0 70 641.0 71 529.0 72 409.0 73 373.0 74 339.0 75 251.0 76 164.5 77 97.0 78 73.5 79 40.5 80 29.5 81 16.5 82 9.0 83 8.0 84 5.5 85 4.0 86 5.0 87 4.0 88 2.5 89 3.5 90 4.0 91 3.5 92 3.0 93 2.0 94 3.0 95 2.5 96 2.0 97 4.5 98 4.0 99 2.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028069854431814163 2 0.014138889639728617 3 0.01289134055387021 4 0.04345629315740119 5 0.03846609681396756 6 0.025366831412454282 7 0.012059641163297937 8 6.237745429292037E-4 9 0.013930964792085549 10-11 2.079248476430679E-4 12-13 0.004678309071969027 14-15 0.017049837506731565 16-17 0.0014554739335014752 18-19 0.009148693296294986 20-21 0.0034307599861106205 22-23 1.0396242382153394E-4 24-25 0.008213031481901181 26-27 5.198121191076697E-4 28-29 0.006861519972221241 30-31 8.316993905722716E-4 32-33 0.0011435866620368733 34-35 3.1188727146460183E-4 36-37 0.006757557548399706 38-39 8.316993905722716E-4 40-41 0.013515115096799412 42-43 0.0076932193627935125 44-45 9.356618143938056E-4 46-47 0.011227941772725666 48-49 0.0030149102908244843 50-51 0.010084355110688793 52-53 0.005613970886362833 54-55 0.0035347224099321543 56-57 0.011331904196547199 58-59 0.018505311440233044 60-61 0.002079248476430679 62-63 0.006653595124578172 64-65 0.0021832109002522127 66-67 0.0015594363573230093 68-69 2.079248476430679E-4 70-71 0.006237745429292037 72-73 5.198121191076697E-4 74-75 0.007069444819864309 76-77 0.015178513877943956 78-79 0.0017673612049660772 80-81 0.004262459376682891 82-83 0.0012475490858584073 84-85 0.0 86-87 0.001975286052609145 88-89 0.005510008462541299 90-91 0.005094158767255164 92-93 0.016218138116159294 94-95 0.0 96-97 6.237745429292037E-4 98-99 0.0031188727146460185 100-101 0.0 102-103 1.0396242382153394E-4 104-105 0.010396242382153394 106-107 0.0 108-109 0.0 110-111 5.198121191076697E-4 112-113 0.007277369667507376 114-115 0.0028069854431814166 116-117 3.1188727146460183E-4 118-119 0.006237745429292037 120-121 0.0021832109002522127 122-123 0.0 124-125 0.07807578028997199 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 480943.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.68046811716142 #Duplication Level Percentage of deduplicated Percentage of total 1 67.14639178806556 27.315466503168302 2 13.219647747203616 10.75562917400443 3 6.168789828364709 7.528477738027806 4 3.5561711348062053 5.786668258746143 5 2.2602806692527624 4.597463785068664 6 1.5926287573298856 3.8873330031019697 7 1.148217227023666 3.2697010006858216 8 0.8355170062089624 2.719137834594388 9 0.6291386017658466 2.3034287547369936 >10 3.27546015120714 22.639100726698715 >50 0.11558872580007973 3.1806453325829174 >100 0.04807672666008563 3.2486816394878035 >500 0.002045818155748325 0.4899862470215049 >1k 0.0015343636168112438 1.0852519169699233 >5k 5.114545389370812E-4 1.1930280851045896 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5734 1.1922410763853513 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2232 0.4640882599393275 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1951 0.4056613777516254 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1033 0.21478636761528913 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 705 0.14658701758836287 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 559 0.11622998983247496 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 547 0.11373489166075813 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 544 0.11311111711782894 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.079248476430679E-4 0.0 10-11 0.0 0.0 0.0 2.079248476430679E-4 0.0 12-13 0.0 0.0 0.0 3.1188727146460183E-4 0.0 14-15 0.0 0.0 0.0 4.158496952861358E-4 0.0 16-17 0.0 0.0 0.0 8.316993905722716E-4 0.0 18-19 0.0 0.0 0.0 0.0010396242382153394 0.0 20-21 0.0 0.0 0.0 0.0021832109002522127 0.0 22-23 0.0 0.0 0.0 0.004990196343433629 0.0 24-25 0.0 0.0 0.0 0.010084355110688791 0.0 26-27 0.0 0.0 0.0 0.015906250844694693 0.0 28-29 0.0 0.0 0.0 0.024223244750417407 0.0 30-31 0.0 0.0 0.0 0.05062970040108703 0.0 32-33 0.0 0.0 0.0 0.0838976760239779 0.0 34-35 0.0 0.0 0.0 0.12080433648062244 0.0 36-37 0.0 0.0 0.0 0.16706761508120505 0.0 38-39 0.0 0.0 0.0 0.2478464183905369 0.0 40-41 0.0 0.0 0.0 0.34848204464978183 0.0 42-43 0.0 0.0 0.0 0.44028086488419627 0.0 44-45 0.0 0.0 0.0 0.5364461069191151 0.0 46-47 0.0 0.0 0.0 0.6463343888984765 0.0 48-49 0.0 0.0 0.0 0.7717130720272465 0.0 50-51 0.0 0.0 0.0 0.920171413244397 0.0 52-53 0.0 0.0 0.0 1.0658227690183661 0.0 54-55 0.0 0.0 0.0 1.201701656953111 0.0 56-57 0.0 0.0 0.0 1.350471885441726 0.0 58-59 0.0 0.0 0.0 1.5281436677527274 0.0 60-61 0.0 0.0 0.0 1.7327417178335063 0.0 62-63 0.0 0.0 0.0 1.9379635424572141 0.0 64-65 0.0 0.0 0.0 2.1351802604466643 0.0 66-67 0.0 0.0 0.0 2.339362460832157 0.0 68-69 0.0 0.0 0.0 2.5739016889735375 0.0 70-71 0.0 0.0 0.0 2.8585508053968973 0.0 72-73 0.0 0.0 0.0 3.151620878149801 0.0 74-75 0.0 0.0 0.0 3.4227548794763623 0.0 76-77 0.0 0.0 0.0 3.7059485219662207 0.0 78-79 0.0 0.0 0.0 4.039148090314237 0.0 80-81 0.0 0.0 0.0 4.414764327581439 0.0 82-83 0.0 0.0 0.0 4.831237797410504 0.0 84-85 0.0 0.0 0.0 5.218289901298075 0.0 86-87 0.0 0.0 0.0 5.602638982166286 0.0 88-89 0.0 0.0 0.0 6.035746439806796 0.0 90-91 0.0 0.0 0.0 6.5456821286514195 0.0 92-93 0.0 0.0 0.0 7.121529994198896 0.0 94-95 0.0 0.0 0.0 7.682511233139894 0.0 96-97 0.0 0.0 0.0 8.210744308577109 0.0 98-99 0.0 0.0 0.0 8.768398749955816 0.0 100-101 0.0 0.0 0.0 9.410678604325252 0.0 102-103 0.0 0.0 0.0 10.097142488818841 0.0 104-105 0.0 0.0 0.0 10.820720958616718 0.0 106-107 0.0 0.0 0.0 11.522883169107358 0.0 108-109 0.0 0.0 0.0 12.200302322728472 0.0 110-111 0.0 0.0 0.0 12.96858463476961 0.0 112-113 0.0 0.0 0.0 13.780843052087253 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 990 0.0 70.35642 1 GTATCAA 1975 0.0 64.50594 1 ATCAACG 2620 0.0 46.348614 3 GAACAAA 1170 0.0 46.302944 1 TCAACGC 2620 0.0 46.121414 4 TATCAAC 2785 0.0 45.740032 2 GTGGTAC 135 2.7284841E-11 44.075115 6 CAACGCA 2765 0.0 43.702755 5 AACGCAG 2740 0.0 43.64884 6 ACGCAAA 180 0.0 42.99559 1 GTACATG 4705 0.0 38.718174 1 CGCAGAG 3165 0.0 37.219757 8 ACGCAGA 3185 0.0 37.172836 7 TACATGG 4760 0.0 36.891216 2 GGTGGTA 165 2.3464963E-10 36.07646 5 GTCGGGA 235 0.0 35.462395 2 GTATGGT 170 3.237801E-10 35.01903 1 CTAGACT 105 1.4774423E-5 34.014954 4 ATGGGAG 1315 0.0 33.950283 5 CATGGGA 3555 0.0 33.656143 4 >>END_MODULE