FastQCFastQC Report
Fri 27 May 2016
SRR940159_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940159_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480943
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103742.1570123694491863No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74441.5477925658549974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58241.2109543126732274No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23930.4975641604098614No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21600.4491176709090266No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17600.3659477318517995No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15060.31313482055046027No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11390.23682640146545433No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9610.19981577858498825No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7990.16613195326681124No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7970.1657161035715251No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT7550.15698325997051626No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5610.11664583952776107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24000.090.3228761
GTATCAA51150.064.734651
TGACGTA300.00423218459.5101935
ATCAACG69400.047.7065243
TATCAAC69650.047.5352862
GTGGTAT4650.047.386691
TCAACGC69900.047.3652734
CAACGCA71100.046.53685
AACGCAG72200.045.823036
ACGCAGA80150.041.0508967
CGCAGAG80350.040.8006178
GCAGAGT83850.038.4589359
GTACACG650.001929240236.6483351
GTAAGGT1652.3464963E-1036.0893024
GTACATG37250.035.971941
CTCCCGC1001.0611719E-535.698697
ATTCGCG501.0622751E-535.6986980-81
CGATCGT501.0622751E-535.6986924-25
ACATGGG38450.033.6066933
TACATGG39250.033.2251432
Produced by FastQC (version 0.11.5)