##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940159_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 480943 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.706295756461785 34.0 31.0 34.0 31.0 34.0 2 32.74680159603113 34.0 31.0 34.0 31.0 34.0 3 32.8417983004223 34.0 31.0 34.0 31.0 34.0 4 36.280505174209836 37.0 37.0 37.0 35.0 37.0 5 36.26879692603905 37.0 37.0 37.0 35.0 37.0 6 36.33995920514489 37.0 37.0 37.0 35.0 37.0 7 36.31231143815379 37.0 37.0 37.0 35.0 37.0 8 36.30821116015827 37.0 37.0 37.0 35.0 37.0 9 38.100427701411604 39.0 39.0 39.0 35.0 39.0 10-11 38.083194890038946 39.0 39.0 39.0 35.0 39.0 12-13 38.074037879748744 39.0 39.0 39.0 35.0 39.0 14-15 39.455301355877936 41.0 39.0 41.0 36.0 41.0 16-17 39.46916890359149 41.0 39.0 41.0 36.5 41.0 18-19 39.586601738667575 41.0 40.0 41.0 37.0 41.0 20-21 39.58119569262885 41.0 40.0 41.0 36.0 41.0 22-23 39.499439642535606 41.0 39.5 41.0 36.0 41.0 24-25 39.43596330542289 41.0 39.0 41.0 35.5 41.0 26-27 39.271598713361044 41.0 39.0 41.0 35.0 41.0 28-29 39.107929629914565 41.0 39.0 41.0 35.0 41.0 30-31 38.72693957496003 40.5 38.0 41.0 35.0 41.0 32-33 38.299045832874164 40.0 36.5 41.0 35.0 41.0 34-35 38.03664363552437 40.0 36.0 41.0 34.5 41.0 36-37 37.810123029132356 40.0 35.5 41.0 34.0 41.0 38-39 37.59354434933037 40.0 35.0 41.0 33.0 41.0 40-41 37.375482125740476 40.0 35.0 41.0 33.0 41.0 42-43 37.075003690666044 40.0 35.0 41.0 33.0 41.0 44-45 36.86609327924515 40.0 35.0 41.0 32.5 41.0 46-47 36.235394838889434 40.0 35.0 41.0 29.0 41.0 48-49 35.667016673493535 40.0 35.0 41.0 24.5 41.0 50-51 35.444533967642734 40.0 35.0 41.0 24.0 41.0 52-53 35.33933335135349 40.0 35.0 41.0 24.0 41.0 54-55 35.16188300900522 39.5 35.0 41.0 24.0 41.0 56-57 34.980050650492885 39.0 35.0 41.0 23.5 41.0 58-59 34.76314344943164 39.0 35.0 41.0 23.0 41.0 60-61 34.52351214177148 38.5 35.0 41.0 22.5 41.0 62-63 34.27835003316402 38.0 35.0 41.0 22.0 41.0 64-65 33.97865859363792 37.0 35.0 41.0 21.0 41.0 66-67 33.66246311933015 37.0 35.0 40.0 19.5 41.0 68-69 33.313802259311394 36.0 34.0 39.5 17.5 41.0 70-71 33.02426690896842 36.0 34.0 39.0 13.5 41.0 72-73 32.81852735147408 35.0 34.0 39.0 10.0 41.0 74-75 32.52111684752663 35.0 34.0 38.0 7.5 40.5 76-77 32.1928627716798 35.0 34.0 37.0 6.5 39.5 78-79 31.876378281833816 35.0 34.0 37.0 2.0 39.0 80-81 31.580664444643126 35.0 34.0 36.0 2.0 39.0 82-83 31.31015005936254 35.0 34.0 36.0 2.0 37.0 84-85 31.083335239311104 35.0 34.0 36.0 2.0 37.0 86-87 30.871903739112533 35.0 34.0 35.0 2.0 37.0 88-89 30.686695720698708 35.0 33.5 35.0 2.0 36.0 90-91 30.537604248320488 35.0 33.0 35.0 2.0 36.0 92-93 30.395857721185255 35.0 33.0 35.0 2.0 36.0 94-95 30.26849439538573 35.0 33.0 35.0 2.0 36.0 96-97 30.17265663498585 35.0 33.0 35.0 2.0 35.0 98-99 30.080470034910583 35.0 33.0 35.0 2.0 35.0 100-101 30.011717604788924 35.0 33.0 35.0 2.0 35.0 102-103 29.2598561575904 34.5 31.5 35.0 2.0 35.0 104-105 29.792766502475345 35.0 33.0 35.0 2.0 35.0 106-107 29.814501926423713 35.0 33.0 35.0 2.0 35.0 108-109 29.76737783895389 35.0 33.0 35.0 2.0 35.0 110-111 29.69003915224881 35.0 33.0 35.0 2.0 35.0 112-113 29.589115550075583 35.0 33.0 35.0 2.0 35.0 114-115 29.47584433082507 35.0 33.0 35.0 2.0 35.0 116-117 29.354164630735866 35.0 33.0 35.0 2.0 35.0 118-119 29.225445634929713 35.0 33.0 35.0 2.0 35.0 120-121 29.0738653021252 35.0 32.0 35.0 2.0 35.0 122-123 28.87347357171224 35.0 32.0 35.0 2.0 35.0 124-125 27.317741395549994 33.5 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2201251388262051 1101 2 0.19896654683326886 1101 3 0.16170955134365528 1101 4 0.13956877898527864 1101 5 0.13740699660822742 1101 6 0.19724313439645158 1101 7 0.2188245630113883 1101 8 0.17196234457448867 1101 9 0.23999027916799065 1101 10-11 0.2528069167811893 1101 12-13 0.2828509833332973 1101 14-15 0.24652452969574767 1101 16-17 0.25294313611756536 1101 18-19 0.24708401700027594 1101 20-21 0.4147835377236717 1101 22-23 0.3852482775934618 1101 24-25 0.34358636073491056 1101 26-27 0.4095691945667568 1101 28-29 0.524825160250856 1101 30-31 0.6421911250452155 1101 32-33 0.5539427869004996 1101 34-35 0.6475694014743922 1101 36-37 0.7304126011695544 1101 38-39 0.7599677108658014 1101 40-41 0.6098573339241895 1101 42-43 0.6214850311047257 1101 44-45 0.739599774079899 1101 46-47 0.8678997300818381 1101 48-49 1.1056725235155227 1101 50-51 1.0214579144690674 1101 52-53 0.9500005208424511 1101 54-55 0.7706347092111301 1101 56-57 0.8852551656329055 1101 58-59 0.8322530270137989 1101 60-61 0.8069077597198699 1101 62-63 0.837620393351088 1101 64-65 0.9348895297667354 1101 66-67 0.9276042605574517 1101 68-69 0.7779375366901533 1101 70-71 0.7555605910595844 1101 72-73 0.8721645955480284 1101 74-75 0.8107970296452365 1101 76-77 0.9472845307723361 1101 78-79 0.9680656028201007 1101 80-81 0.9156829355551608 1101 82-83 0.9734953448980086 1101 84-85 0.9041367852117439 1101 86-87 0.9331115788039455 1101 88-89 0.9403558651359241 1101 90-91 0.9386429132404217 1101 92-93 0.969677617395444 1101 94-95 1.0105437652885065 1101 96-97 0.8839246277254951 1101 98-99 1.0419526361188076 1101 100-101 1.0047254585137964 1101 102-103 1.0640869974001248 1101 104-105 1.0897895537059448 1101 106-107 1.1367972661300705 1101 108-109 1.1963999762417323 1101 110-111 1.1901597318921056 1101 112-113 1.1579605858667996 1101 114-115 1.2196003196724874 1101 116-117 1.107949310197636 1101 118-119 1.1164696622036132 1101 120-121 1.2483166076888352 1101 122-123 1.1812663563432153 1101 124-125 1.0552198009170297 1102 1 0.07850484480452735 1102 2 0.08791030879388728 1102 3 0.12624219445213214 1102 4 0.1695485837351356 1102 5 0.07177904779305067 1102 6 0.22665853040854955 1102 7 0.19092381076036702 1102 8 0.037447868372254334 1102 9 0.17842342759867336 1102 10-11 0.18690668484448025 1102 12-13 0.1364552622570443 1102 14-15 0.15700952436031912 1102 16-17 0.2542780950656436 1102 18-19 0.18707480126385434 1102 20-21 0.18725516295626932 1102 22-23 0.25957693985656505 1102 24-25 0.21909454907105186 1102 26-27 0.2820293552468769 1102 28-29 0.24885574414933131 1102 30-31 0.22546015399761643 1102 32-33 0.039719025292647814 1102 34-35 0.07430319456567247 1102 36-37 0.08584900376936133 1102 38-39 -0.04522198338339933 1102 40-41 -0.10748302218244987 1102 42-43 0.09874994163712358 1102 44-45 0.1901798363412368 1102 46-47 0.2474238188703879 1102 48-49 0.008650441023647204 1102 50-51 -0.010185352752017707 1102 52-53 0.2782648316905636 1102 54-55 0.248988752229657 1102 56-57 0.29416598905234537 1102 58-59 0.32890956665910664 1102 60-61 -0.11775690211992895 1102 62-63 -0.011953654429639471 1102 64-65 -0.07552557522210179 1102 66-67 -0.14889186354192674 1102 68-69 -0.14102240792723109 1102 70-71 -0.049492410448451096 1102 72-73 -0.15821316145636644 1102 74-75 -0.06943036534386948 1102 76-77 -0.2719386576078975 1102 78-79 -0.18681829653876392 1102 80-81 -0.14819115720188591 1102 82-83 -0.1814458772232861 1102 84-85 -0.06478104922247852 1102 86-87 -0.14707083986387204 1102 88-89 -0.06996664154437937 1102 90-91 -0.06652715898626127 1102 92-93 -0.3205453240559706 1102 94-95 -0.30486701844593966 1102 96-97 -0.43596063856078615 1102 98-99 -0.3657588187329033 1102 100-101 -0.4841263665547686 1102 102-103 -0.45121266836407514 1102 104-105 -0.40437675285618724 1102 106-107 -0.41930077343145555 1102 108-109 -0.3891652702902384 1102 110-111 -0.3285826067002233 1102 112-113 -0.29362813654111264 1102 114-115 -0.2494398768123034 1102 116-117 -0.3328858824509382 1102 118-119 -0.41615139906119225 1102 120-121 -0.3524843676800131 1102 122-123 -0.2969642222639308 1102 124-125 -0.2192393808666786 1103 1 -0.023709649866518134 1103 2 0.11780565162823109 1103 3 -0.002072898930300937 1103 4 -0.03827734969816987 1103 5 0.05961038951891595 1103 6 0.10002179558696866 1103 7 0.013762002245215399 1103 8 0.21804014345321576 1103 9 4.0344373415734935E-4 1103 10-11 -0.005060883278780182 1103 12-13 0.09877376599256849 1103 14-15 0.07560801674289763 1103 16-17 0.0725516429279196 1103 18-19 0.03701591345016908 1103 20-21 0.03161701795592364 1103 22-23 0.06361461017867498 1103 24-25 0.04841040006141384 1103 26-27 0.024467243688548024 1103 28-29 0.09255161932057376 1103 30-31 -0.25198145543873807 1103 32-33 -0.2861149580042266 1103 34-35 -0.4012332208984617 1103 36-37 -0.4227908553468467 1103 38-39 -0.07924952991817946 1103 40-41 0.04773385705395583 1103 42-43 0.1328568246950823 1103 44-45 0.07157732126170657 1103 46-47 -0.36886005838372427 1103 48-49 -0.5124262561633657 1103 50-51 -0.6258904086116317 1103 52-53 -0.8150047493455475 1103 54-55 -0.8626556145419713 1103 56-57 -0.830271671436499 1103 58-59 -0.8924080453785876 1103 60-61 -0.5723685889484997 1103 62-63 -0.7693258052986494 1103 64-65 -0.8956875558193005 1103 66-67 -0.9143094083111905 1103 68-69 -0.8795649848222027 1103 70-71 -0.61398294705387 1103 72-73 -0.6374728064310133 1103 74-75 -0.5671765376359801 1103 76-77 -0.7021605093447008 1103 78-79 -0.8370805540315978 1103 80-81 -0.666558121026668 1103 82-83 -0.7275191662920548 1103 84-85 -0.7493937864219227 1103 86-87 -0.8563773492579898 1103 88-89 -0.8978175603954099 1103 90-91 -0.7450935591865004 1103 92-93 -0.8562221763403031 1103 94-95 -0.7390802794899187 1103 96-97 -0.7746739565929737 1103 98-99 -0.8040227121224923 1103 100-101 -0.8074729822374778 1103 102-103 -0.757475113582764 1103 104-105 -0.8038026246081422 1103 106-107 -0.8623315627069417 1103 108-109 -0.8725213616590963 1103 110-111 -0.9033656604188707 1103 112-113 -0.8153931618729295 1103 114-115 -0.8402969570941607 1103 116-117 -0.677546087474397 1103 118-119 -0.683385861952928 1103 120-121 -0.7528566906310665 1103 122-123 -0.8028049547523466 1103 124-125 -0.8537717758687045 1104 1 0.05206111783688527 1104 2 -0.019868510325139255 1104 3 0.08567012498028248 1104 4 -0.006879010978757094 1104 5 -0.15058705998796285 1104 6 0.035330000901893754 1104 7 -0.0771832722867174 1104 8 -0.020966254263598216 1104 9 0.08643226851270214 1104 10-11 0.12833343453554846 1104 12-13 0.14713657013287929 1104 14-15 0.2299492599669435 1104 16-17 0.11701566170721378 1104 18-19 0.12782663013466333 1104 20-21 0.1316274207474919 1104 22-23 0.12044777427819042 1104 24-25 0.13371102943030877 1104 26-27 0.1017113632844655 1104 28-29 0.13380094935818931 1104 30-31 -0.0017434350447373959 1104 32-33 0.14688767209286624 1104 34-35 0.21670051331925322 1104 36-37 0.00957856135053703 1104 38-39 0.07270698641307405 1104 40-41 -0.16066458795485516 1104 42-43 -0.18725516361760697 1104 44-45 -0.13286580147541116 1104 46-47 -0.17530707538323753 1104 48-49 -0.2125951884524966 1104 50-51 -0.3058632256649716 1104 52-53 -0.29011946273733 1104 54-55 -0.2041102866391924 1104 56-57 -0.27916004269856387 1104 58-59 -0.16697615991785142 1104 60-61 -0.42287708192322526 1104 62-63 -0.3568766037129052 1104 64-65 -0.3555474799693812 1104 66-67 -0.3434731821725947 1104 68-69 -0.19807132487137125 1104 70-71 -0.3241566510582672 1104 72-73 -0.3554305122988666 1104 74-75 -0.3749533027748697 1104 76-77 -0.25913162350629193 1104 78-79 -0.2273684173660513 1104 80-81 -0.3296241403080167 1104 82-83 -0.2485769040467254 1104 84-85 -0.29349031528175473 1104 86-87 -0.17579393356922424 1104 88-89 -0.1640374347025535 1104 90-91 -0.16812194750009724 1104 92-93 -0.2832940845062666 1104 94-95 -0.2040601862718603 1104 96-97 -0.08377707765659181 1104 98-99 -0.10260883706002133 1104 100-101 -0.04632333808079636 1104 102-103 0.017593917985184504 1104 104-105 -1.869760958541633E-4 1104 106-107 0.009338695938112096 1104 108-109 0.029444765946973206 1104 110-111 0.11893928158556477 1104 112-113 0.11123436617503302 1104 114-115 0.138116119667135 1104 116-117 0.10578411748321415 1104 118-119 0.19675023369847722 1104 120-121 0.272794105747856 1104 122-123 0.21504827090455336 1104 124-125 0.31703075427112637 1105 1 -0.06382167343163303 1105 2 0.029329350637425478 1105 3 0.1159892272858869 1105 4 0.020354266833116696 1105 5 -0.08608719148617183 1105 6 0.13757423689732917 1105 7 -0.08924165748878465 1105 8 0.07369711033708626 1105 9 -0.08228869230083546 1105 10-11 0.07253217215279761 1105 12-13 0.16923703475985974 1105 14-15 0.06051272976619515 1105 16-17 0.21128234006261692 1105 18-19 0.2601916253130696 1105 20-21 0.2817085113059221 1105 22-23 0.062123938037281334 1105 24-25 0.06299020011497447 1105 26-27 0.03193622521135353 1105 28-29 -0.13989393097845237 1105 30-31 -0.3641623444863029 1105 32-33 -0.08434534272260663 1105 34-35 -0.13544838647911206 1105 36-37 0.049208832236828925 1105 38-39 0.09436802787883636 1105 40-41 -0.01526154596309226 1105 42-43 -0.02360126688752473 1105 44-45 0.013573884682223536 1105 46-47 -0.1364636020773773 1105 48-49 -0.07187238666412554 1105 50-51 -0.022703762177641806 1105 52-53 -0.10659070846364216 1105 54-55 0.14531769617837398 1105 56-57 0.1855633727480921 1105 58-59 -0.11985158728701606 1105 60-61 0.07346288476389162 1105 62-63 0.12654695199849186 1105 64-65 0.11792933251417992 1105 66-67 0.16720447189947407 1105 68-69 -0.04427979335563492 1105 70-71 -0.09364687635851965 1105 72-73 -0.09454903601237419 1105 74-75 -0.15619398831884013 1105 76-77 -0.21083452523330237 1105 78-79 -0.07023573567936836 1105 80-81 0.0128826537658 1105 82-83 -0.08980159064267568 1105 84-85 -0.004585476147887846 1105 86-87 -0.007039002386616033 1105 88-89 -0.05551415952064431 1105 90-91 -0.09673486062361647 1105 92-93 -0.16925774716954223 1105 94-95 0.05540110942376231 1105 96-97 -0.0731509270473154 1105 98-99 0.007389409630608412 1105 100-101 0.020144620190443163 1105 102-103 0.04013490159174182 1105 104-105 -0.008735455099973422 1105 106-107 0.009446086137117504 1105 108-109 -0.12067029498052761 1105 110-111 -0.12256922196488418 1105 112-113 -0.2588234930342246 1105 114-115 -0.23216966976040965 1105 116-117 -0.0505968328104629 1105 118-119 -0.05415819971959834 1105 120-121 -0.1381575029134936 1105 122-123 0.05798791322944297 1105 124-125 -0.01948520084415506 1106 1 0.01729962294511722 1106 2 -0.02926826861706644 1106 3 -0.10131462187563756 1106 4 -0.06249828334500762 1106 5 -0.17534342573762274 1106 6 -0.0020643068510395324 1106 7 0.06392977356517093 1106 8 -0.11808975211838657 1106 9 0.11574762710137065 1106 10-11 0.048870255392003514 1106 12-13 0.035889220006190214 1106 14-15 0.005954340679394932 1106 16-17 0.04596823805549377 1106 18-19 0.080813529909463 1106 20-21 0.17771815593186346 1106 22-23 -0.028405213061859058 1106 24-25 0.010530168835103382 1106 26-27 0.031108612039055572 1106 28-29 0.12581225548431973 1106 30-31 0.2474492203759695 1106 32-33 0.2660521722656668 1106 34-35 0.2896091771667315 1106 36-37 0.11809513860576004 1106 38-39 0.18913190832022764 1106 40-41 0.19913262381707852 1106 42-43 0.3607848657043604 1106 44-45 0.40980183671975823 1106 46-47 0.26527742668272225 1106 48-49 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-0.1256968137847707 2216 110-111 -0.09685803585109554 2216 112-113 -0.17018639885229092 2216 114-115 -0.30187394491309405 2216 116-117 -0.11239770069860455 2216 118-119 -0.08536601071284622 2216 120-121 -0.021145288191533496 2216 122-123 -0.3459055293935087 2216 124-125 -0.6525171841292732 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 18.0 5 67.0 6 170.0 7 307.0 8 606.0 9 1121.0 10 2262.0 11 3045.0 12 3331.0 13 5108.0 14 9123.0 15 9375.0 16 3467.0 17 2711.0 18 2570.0 19 2453.0 20 2328.0 21 2319.0 22 2451.0 23 2223.0 24 2432.0 25 2797.0 26 3295.0 27 3861.0 28 4525.0 29 5273.0 30 6532.0 31 8013.0 32 10009.0 33 13080.0 34 19125.0 35 40455.0 36 63015.0 37 148378.0 38 94651.0 39 441.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.89862426820011 18.844510071022285 14.758077095053396 19.498788565724208 2 15.572412100947972 22.558798638445815 40.449262976663526 21.419526283942687 3 17.118463979240264 30.617697757058703 32.25244369774458 20.011394565956447 4 11.046439035759473 18.42890557368316 38.585436505294 31.939218885263372 5 10.821921226311128 40.177409226444205 33.18188812322996 15.818781424014706 6 26.426638770633463 39.65098069392076 17.95737813336439 15.965002402081389 7 23.779533125547104 37.66038802935067 22.099916206286398 16.460162638815827 8 26.875742031800026 32.64170598178994 20.626976585582906 19.855575400827124 9 28.722322603718116 14.368646596374207 20.638620377050916 36.27041042285676 10-11 23.842638316806774 27.938238003256107 26.961199144181325 21.257924535755794 12-13 26.998729579180903 26.45022383109849 28.41043533225351 18.1406112574671 14-15 23.045766338214715 25.162753174492615 30.22042113098642 21.571059356306257 16-17 18.872090871475415 29.10324092460021 29.91175669466028 22.11291150926409 18-19 19.26974714259278 24.138411412579035 37.035989711878536 19.55585173294964 20-21 20.738424303919594 23.139644406925562 37.57004468304976 18.55188660610509 22-23 20.09312953925933 23.84773247557403 37.57586657878376 18.483271406382876 24-25 19.440447625265627 23.86263925911448 37.95655573067631 18.74035738494359 26-27 19.34158517745346 23.923832969811393 37.65550179543106 19.079080057304086 28-29 19.36174725224457 23.928552121063422 37.66566584466189 19.044034782030117 30-31 19.11117487983581 23.720198085066784 37.981761451597976 19.186865583499426 32-33 19.130333532248102 24.00565139735894 37.44248278902074 19.42153228137222 34-35 19.63569487444458 23.816231861156105 37.2853955666264 19.26267769777292 36-37 19.735580871666173 23.616306366061536 36.77561280905558 19.872499953216717 38-39 19.582258188600314 23.604356441407816 36.71661714589879 20.096768224093083 40-41 19.667923225829256 23.753230632320253 36.6321996577557 19.94664648409479 42-43 19.99145428876187 23.63845611642128 36.136194933703166 20.233894661113688 44-45 19.694293315811308 23.85747273186621 35.79795745017087 20.650276502151613 46-47 20.64721978483534 24.243867141853144 34.172776306104375 20.93613676720714 48-49 20.656501913948222 24.723927783541917 32.91315187038797 21.706418432121893 50-51 20.787809414497165 24.868249646257187 32.877028497999184 21.466912441246464 52-53 20.944789715205335 24.996309333954336 32.73392065171964 21.324980299120686 54-55 20.449611790587323 25.42875619118118 32.26244786100148 21.85918415723001 56-57 20.671372030427882 25.40407805123503 32.0941987626328 21.830351155704292 58-59 20.766631665401768 24.518490024639505 32.55242392423093 22.162454385727802 60-61 20.707547464044595 24.96127399712648 32.64742391510013 21.6837546237288 62-63 21.24474183802262 24.860943318937284 32.600500292148986 21.293814550891106 64-65 20.929635363113796 25.00985069568264 32.93500828082228 21.125505660381283 66-67 20.92796955977066 25.447740634275405 32.65795807191088 20.96633173404305 68-69 21.28336441835659 25.071682687409425 32.800313553278066 20.844639340955922 70-71 20.992063244525678 25.235846604578242 32.483932072850976 21.288158078045107 72-73 21.06820452520489 25.380903814184435 31.93021415865352 21.620677501957154 74-75 20.70650783980638 25.73943273943066 31.831838700220196 21.72222072054277 76-77 20.748961652622768 25.58352783394759 32.09909914593004 21.568411367499596 78-79 20.582734418778244 25.49166787090684 32.26351755262356 21.662080157691353 80-81 20.726615683388015 25.609428995596144 31.926095038486967 21.73786028252887 82-83 20.735750487329437 25.27573749187784 31.802053281351526 22.186458739441196 84-85 20.599218618422558 25.12865349947915 31.80595205668863 22.466175825409664 86-87 20.626791467701338 24.995243546037795 31.714891391702043 22.663073594558824 88-89 20.801610248700666 25.052633548857965 31.482815731115082 22.66294047132628 90-91 20.568539444997207 25.531147298959382 31.5249112891739 22.37540196686951 92-93 20.686134559061284 25.189977781774818 31.554558375085644 22.569329284078254 94-95 20.530209617755858 24.956178016023355 31.630947157861026 22.88266520835976 96-97 20.823937328779586 24.760434373521722 31.60748353424928 22.808144763449413 98-99 20.69610836808469 25.05476522431649 31.282561525753433 22.966564881845393 100-101 20.705877704977347 25.0776118223651 31.509672106945246 22.706838365712315 102-103 20.79926309518735 24.842362877796273 31.23601019274095 23.12236383427543 104-105 20.602615937862897 24.87864822071105 31.402378484477683 23.11635735694837 106-107 20.636749053422133 24.966056268622268 31.218772286944606 23.178422391010994 108-109 20.896925234742884 24.894338410288242 30.742349996932827 23.46638635803605 110-111 20.82745499628326 25.110280548725655 30.682268301684744 23.379996153306337 112-113 20.40855367673357 25.348085736386395 30.813801162781022 23.42955942409901 114-115 20.32694103043396 25.63890107559524 30.46722792513874 23.566929968832063 116-117 20.281530536394683 25.38317990196333 30.7020069966784 23.633282564963586 118-119 19.971056289487006 25.679641779996608 30.52064370645838 23.828658224058003 120-121 20.024098489841833 25.856182541382246 30.565992227769197 23.55372674100673 122-123 20.101074580190133 25.92279157968278 30.089908760490047 23.88622507963704 124-125 20.2170867400482 26.02055833894113 29.76424371710078 23.998111203909893 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1957.0 1 1343.0 2 1417.0 3 1892.0 4 1003.5 5 600.0 6 1485.5 7 3653.0 8 6195.0 9 6311.0 10 3667.5 11 1498.0 12 1087.0 13 1085.5 14 1177.5 15 1269.5 16 1313.0 17 1372.5 18 1467.0 19 1479.5 20 1497.0 21 1653.5 22 1770.0 23 1929.5 24 2204.5 25 2663.0 26 3115.5 27 3495.0 28 4041.5 29 4873.0 30 6085.0 31 7221.5 32 8220.0 33 8949.5 34 9685.0 35 10373.5 36 10899.0 37 11656.0 38 12654.5 39 13328.5 40 13373.0 41 13800.0 42 14498.0 43 15538.5 44 16722.0 45 17174.5 46 17072.0 47 16854.5 48 16383.5 49 15787.5 50 15284.5 51 14088.5 52 13111.5 53 12922.5 54 12454.5 55 11877.0 56 10838.5 57 9622.0 58 9336.0 59 8807.5 60 7875.0 61 7171.0 62 6149.5 63 4581.5 64 3174.0 65 2336.0 66 1774.5 67 1357.5 68 958.5 69 740.5 70 608.0 71 470.5 72 349.5 73 329.5 74 309.5 75 236.5 76 154.0 77 87.5 78 62.0 79 30.5 80 14.0 81 6.0 82 2.5 83 2.5 84 1.5 85 0.5 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022455883545451333 2 0.004574346648147493 3 0.0024950981717168147 4 0.08296201420958409 5 0.0043664218005044254 6 0.02287173324073747 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0010396242382153394 26-27 0.0 28-29 0.0010396242382153394 30-31 0.008005106634258114 32-33 0.0 34-35 0.0 36-37 4.158496952861358E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.005094158767255164 46-47 0.0018713236287876112 48-49 0.0 50-51 0.0030149102908244843 52-53 0.0 54-55 0.0035347224099321543 56-57 5.198121191076697E-4 58-59 0.001663398781144543 60-61 0.0 62-63 0.004990196343433629 64-65 0.0025990605955383485 66-67 1.0396242382153394E-4 68-69 8.316993905722716E-4 70-71 0.003326797562289086 72-73 0.0030149102908244843 74-75 0.0 76-77 0.0021832109002522127 78-79 7.277369667507376E-4 80-81 2.079248476430679E-4 82-83 0.0011435866620368733 84-85 0.0 86-87 0.003638684833753688 88-89 0.004886233919612095 90-91 0.005510008462541299 92-93 0.006549632700756638 94-95 0.004158496952861358 96-97 0.004262459376682891 98-99 0.005510008462541299 100-101 0.004990196343433629 102-103 0.0025990605955383485 104-105 0.0013515115096799414 106-107 0.0 108-109 0.009252655720116522 110-111 0.0021832109002522127 112-113 0.005613970886362833 114-115 0.0 116-117 1.0396242382153394E-4 118-119 0.001975286052609145 120-121 0.0 122-123 0.0022871733240737466 124-125 0.044391954971794996 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 480943.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.7623527157614 #Duplication Level Percentage of deduplicated Percentage of total 1 61.97880824298589 21.54529193045216 2 14.995671727364115 10.425696595928049 3 7.155952647966901 7.462732498977366 4 4.209165369419435 5.852819648429061 5 2.6795636300316756 4.657396801574355 6 1.9060862852690015 3.9756026253117858 7 1.2936747401690665 3.1479824332059656 8 0.9656238060939295 2.6853884270538537 9 0.7990697746239869 2.4999790814984704 >10 3.791720782104133 22.57336980791147 >50 0.14917622799728986 3.476512775610881 >100 0.06769844885017545 4.101297914701064 >500 0.002995506586290949 0.8065976145654405 >1k 0.002995506586290949 1.8656084253233194 >5k 0.0011982026345163798 2.763216408482898 >10k+ 5.991013172581899E-4 2.160507010973891 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10374 2.1570123694491863 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7444 1.5477925658549974 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5824 1.2109543126732274 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2393 0.4975641604098614 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2160 0.4491176709090266 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1760 0.3659477318517995 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1506 0.31313482055046027 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1139 0.23682640146545433 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 961 0.19981577858498825 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 799 0.16613195326681124 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 797 0.1657161035715251 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 755 0.15698325997051626 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 561 0.11664583952776107 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.079248476430679E-4 0.0 10-11 0.0 0.0 0.0 2.079248476430679E-4 0.0 12-13 0.0 0.0 0.0 3.1188727146460183E-4 0.0 14-15 0.0 0.0 0.0 4.158496952861358E-4 0.0 16-17 0.0 0.0 0.0 8.316993905722716E-4 0.0 18-19 0.0 0.0 0.0 0.0010396242382153394 0.0 20-21 0.0 0.0 0.0 0.0024950981717168147 0.0 22-23 0.0 0.0 0.0 0.005406046038719765 0.0 24-25 0.0 0.0 0.0 0.010500204805974928 0.0 26-27 0.0 0.0 0.0 0.01632210053998083 0.0 28-29 0.0 0.0 0.0 0.024639094445703544 0.0 30-31 0.0 0.0 0.0 0.050941587672551636 0.0 32-33 0.0 0.0 0.0 0.08410560087162096 0.0 34-35 0.0 0.0 0.0 0.12153207344737318 0.0 36-37 0.0 0.0 0.0 0.1677953520479558 0.0 38-39 0.0 0.0 0.0 0.24732660627142924 0.0 40-41 0.0 0.0 0.0 0.3487939319212464 0.0 42-43 0.0 0.0 0.0 0.4401769024603747 0.0 44-45 0.0 0.0 0.0 0.5364461069191151 0.0 46-47 0.0 0.0 0.0 0.6470621258652273 0.0 48-49 0.0 0.0 0.0 0.7738962829274987 0.0 50-51 0.0 0.0 0.0 0.9226665114161138 0.0 52-53 0.0 0.0 0.0 1.0696693786997629 0.0 54-55 0.0 0.0 0.0 1.2078354399585813 0.0 56-57 0.0 0.0 0.0 1.3604522781285933 0.0 58-59 0.0 0.0 0.0 1.54186670769717 0.0 60-61 0.0 0.0 0.0 1.7512470292737392 0.0 62-63 0.0 0.0 0.0 1.964577922955527 0.0 64-65 0.0 0.0 0.0 2.1658491754740163 0.0 66-67 0.0 0.0 0.0 2.373877985540906 0.0 68-69 0.0 0.0 0.0 2.611432123973111 0.0 70-71 0.0 0.0 0.0 2.9008635118922617 0.0 72-73 0.0 0.0 0.0 3.1995475555315287 0.0 74-75 0.0 0.0 0.0 3.4748400538109507 0.0 76-77 0.0 0.0 0.0 3.761568418710741 0.0 78-79 0.0 0.0 0.0 4.099134408859262 0.0 80-81 0.0 0.0 0.0 4.48192405337015 0.0 82-83 0.0 0.0 0.0 4.905986780138187 0.0 84-85 0.0 0.0 0.0 5.300004366421801 0.0 86-87 0.0 0.0 0.0 5.689447606057266 0.0 88-89 0.0 0.0 0.0 6.129000733974712 0.0 90-91 0.0 0.0 0.0 6.648708890658561 0.0 92-93 0.0 0.0 0.0 7.236408472521692 0.0 94-95 0.0 0.0 0.0 7.805498780520768 0.0 96-97 0.0 0.0 0.0 8.343088474101922 0.0 98-99 0.0 0.0 0.0 8.909475759081637 0.0 100-101 0.0 0.0 0.0 9.559968644932976 0.0 102-103 0.0 0.0 0.0 10.254749523332286 0.0 104-105 0.0 0.0 0.0 10.990179709445819 0.0 106-107 0.0 0.0 0.0 11.704505523523578 0.0 108-109 0.0 0.0 0.0 12.395751679512957 0.0 110-111 0.0 0.0 0.0 13.177653069074713 0.0 112-113 0.0 0.0 0.0 14.00020376635069 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2400 0.0 90.322876 1 GTATCAA 5115 0.0 64.73465 1 TGACGTA 30 0.004232184 59.510193 5 ATCAACG 6940 0.0 47.706524 3 TATCAAC 6965 0.0 47.535286 2 GTGGTAT 465 0.0 47.38669 1 TCAACGC 6990 0.0 47.365273 4 CAACGCA 7110 0.0 46.5368 5 AACGCAG 7220 0.0 45.82303 6 ACGCAGA 8015 0.0 41.050896 7 CGCAGAG 8035 0.0 40.800617 8 GCAGAGT 8385 0.0 38.458935 9 GTACACG 65 0.0019292402 36.648335 1 GTAAGGT 165 2.3464963E-10 36.089302 4 GTACATG 3725 0.0 35.97194 1 CTCCCGC 100 1.0611719E-5 35.69869 7 ATTCGCG 50 1.0622751E-5 35.69869 80-81 CGATCGT 50 1.0622751E-5 35.69869 24-25 ACATGGG 3845 0.0 33.606693 3 TACATGG 3925 0.0 33.225143 2 >>END_MODULE