FastQCFastQC Report
Fri 27 May 2016
SRR940158_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940158_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876379
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43750.4992132399338643No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19520.22273468442306354No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14630.1669369074338842No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9720.11091091867787796No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22950.058.6187321
GGTATCA13700.057.3540421
TATACCG350.00778190351.0227555
TCAACGC27950.046.2156034
ATCAACG28800.044.85163
CAACGCA29450.043.8616685
AACGCAG30050.042.5799876
TATCAAC32050.041.6035772
GTACATG61400.040.5245861
TACATGG64800.037.8471262
ACGCAGA37250.034.341927
GAACAAA12900.034.1470151
CGCAGAG37750.034.048568
ACATGGG70050.033.5660063
ATGGGAT18550.032.0897835
GTGTAAG2307.2759576E-1231.0573271
CATGGGA46150.030.82744
GTATTAC1751.663102E-830.6136511
CATGGGG24650.030.1858774
GCAGAGT42100.028.5516519
Produced by FastQC (version 0.11.5)