##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940158_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 876379 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.18098904697625 33.0 31.0 34.0 28.0 34.0 2 31.26532242328947 34.0 31.0 34.0 27.0 34.0 3 31.44790438839817 34.0 31.0 34.0 27.0 34.0 4 34.52648454606968 37.0 35.0 37.0 31.0 37.0 5 34.78745040673042 37.0 35.0 37.0 31.0 37.0 6 34.63694246439041 37.0 35.0 37.0 31.0 37.0 7 34.598743237800086 37.0 35.0 37.0 31.0 37.0 8 34.44592921555628 37.0 35.0 37.0 31.0 37.0 9 35.93052320970722 39.0 38.0 39.0 30.0 39.0 10-11 36.00189758084116 39.0 37.5 39.0 30.5 39.0 12-13 35.9415173115741 39.0 37.0 39.0 30.5 39.0 14-15 37.35400266323132 41.0 38.0 41.0 31.0 41.0 16-17 37.428497830276626 41.0 38.0 41.0 31.0 41.0 18-19 37.462752987006766 41.0 38.0 41.0 31.0 41.0 20-21 37.51687854227452 41.0 38.0 41.0 31.0 41.0 22-23 37.53068078993221 41.0 38.0 41.0 31.0 41.0 24-25 37.57194946478636 41.0 38.0 41.0 31.0 41.0 26-27 37.55043594152758 41.0 38.0 41.0 31.0 41.0 28-29 37.468181003880744 41.0 38.0 41.0 30.0 41.0 30-31 37.36578523675259 40.5 38.0 41.0 30.0 41.0 32-33 37.27091475263556 40.0 38.0 41.0 30.0 41.0 34-35 37.16917566486646 40.0 38.0 41.0 30.0 41.0 36-37 37.02161564802443 40.0 38.0 41.0 30.0 41.0 38-39 36.85689810002293 40.0 37.5 41.0 30.0 41.0 40-41 36.723205371192144 40.0 37.0 41.0 30.0 41.0 42-43 36.678450191070304 40.0 37.0 41.0 29.5 41.0 44-45 36.739283460694516 40.0 37.0 41.0 30.0 41.0 46-47 36.637467351454106 40.0 37.0 41.0 30.0 41.0 48-49 36.5160923527378 40.0 37.0 41.0 29.0 41.0 50-51 36.38810720019535 40.0 36.0 41.0 29.0 41.0 52-53 36.22011709545756 40.0 36.0 41.0 29.0 41.0 54-55 36.04766316856063 40.0 35.0 41.0 29.0 41.0 56-57 35.850752357142284 40.0 35.0 41.0 28.0 41.0 58-59 35.61907291251844 39.0 35.0 41.0 28.0 41.0 60-61 35.38442614439643 39.0 35.0 41.0 28.0 41.0 62-63 35.10246537171703 39.0 35.0 41.0 28.0 41.0 64-65 34.80678393708658 38.0 35.0 41.0 27.0 41.0 66-67 34.47923615239525 37.0 34.0 40.0 26.0 41.0 68-69 34.155627873328775 37.0 34.0 40.0 26.0 41.0 70-71 34.03207858700402 36.0 34.0 39.0 26.5 41.0 72-73 33.79246935401236 36.0 34.0 39.0 26.5 41.0 74-75 33.460058376569954 35.5 34.0 39.0 26.0 41.0 76-77 33.10779411647244 35.0 34.0 37.0 26.0 39.5 78-79 32.77988518666011 35.0 34.0 37.0 26.0 39.0 80-81 32.47183866797356 35.0 34.0 36.5 25.0 39.0 82-83 32.188409352574624 35.0 34.0 36.0 25.0 37.5 84-85 31.924079079941443 35.0 34.0 36.0 25.0 37.0 86-87 31.719656678217987 35.0 34.0 35.0 24.0 37.0 88-89 31.540881855909372 35.0 33.5 35.0 24.0 36.0 90-91 31.404945234881254 35.0 33.0 35.0 24.0 36.0 92-93 31.260689724422882 35.0 33.0 35.0 23.0 36.0 94-95 31.159048197184095 35.0 33.0 35.0 21.0 36.0 96-97 31.038881009243717 35.0 33.0 35.0 20.0 35.5 98-99 30.95464519346082 35.0 33.0 35.0 19.0 35.0 100-101 30.88391552056816 35.0 33.0 35.0 18.5 35.0 102-103 30.13942769053115 34.5 31.5 35.0 17.0 35.0 104-105 30.687076025326945 35.0 33.0 35.0 17.0 35.0 106-107 30.734991938419334 35.0 33.0 35.0 16.0 35.0 108-109 30.690324049298304 35.0 33.0 35.0 12.5 35.0 110-111 30.598582919033888 35.0 33.0 35.0 7.0 35.0 112-113 30.4903626170869 35.0 33.0 35.0 2.0 35.0 114-115 30.369766961554305 35.0 33.0 35.0 2.0 35.0 116-117 30.28465652417504 35.0 33.0 35.0 2.0 35.0 118-119 30.149542606566335 35.0 33.0 35.0 2.0 35.0 120-121 29.99235661739955 35.0 32.5 35.0 2.0 35.0 122-123 29.776450599569365 35.0 32.0 35.0 2.0 35.0 124-125 27.827592856515274 33.0 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22787445057916855 1101 2 0.14587914122725465 1101 3 0.12009544823509799 1101 4 0.10166927470908149 1101 5 0.14989106231737992 1101 6 0.3402049660354649 1101 7 0.08867498022827647 1101 8 0.3532000201870318 1101 9 0.21427305505552852 1101 10-11 0.34645141139038316 1101 12-13 0.17694791627398132 1101 14-15 0.3168452758600253 1101 16-17 0.26750250085696337 1101 18-19 0.25373576381113594 1101 20-21 0.14959896845603282 1101 22-23 0.1250648973730648 1101 24-25 0.2395571410061592 1101 26-27 0.2636955359795792 1101 28-29 0.3255886555326626 1101 30-31 0.30724025108085584 1101 32-33 0.26877187230711286 1101 34-35 0.38771010367622694 1101 36-37 0.3351265375901704 1101 38-39 0.3322565445773549 1101 40-41 0.38505378747215957 1101 42-43 0.3828484271649941 1101 44-45 0.3436599338874444 1101 46-47 0.3243271511332537 1101 48-49 0.33020377785505417 1101 50-51 0.22445159662046166 1101 52-53 0.2820177564316708 1101 54-55 0.28248247590538256 1101 56-57 0.3321081187020525 1101 58-59 0.3242357047884994 1101 60-61 0.294613135454739 1101 62-63 0.3637351432017937 1101 64-65 0.3154000363591365 1101 66-67 0.3535196489655874 1101 68-69 0.24355306449224656 1101 70-71 0.30046548584421373 1101 72-73 0.27506587167345486 1101 74-75 0.19710288876579085 1101 76-77 0.352768876815432 1101 78-79 0.311086011648527 1101 80-81 0.3252522463741201 1101 82-83 0.23222787472860773 1101 84-85 0.18201528903928477 1101 86-87 0.225872894267372 1101 88-89 0.21302320512434747 1101 90-91 0.1800262419135379 1101 92-93 0.30442023348394187 1101 94-95 0.2606620666855015 1101 96-97 0.21197821210198597 1101 98-99 0.2168306598913361 1101 100-101 0.23972099319230722 1101 102-103 0.3728949955467513 1101 104-105 0.3001070384240876 1101 106-107 0.2832122261331129 1101 108-109 0.22992589762375815 1101 110-111 0.1724421473729869 1101 112-113 0.21580451721282756 1101 114-115 0.31087832760377054 1101 116-117 0.33832593894951657 1101 118-119 0.3669225541093759 1101 120-121 0.2281741958295065 1101 122-123 0.18680354178950154 1101 124-125 0.0072444147162720185 1102 1 0.3515780616386479 1102 2 0.4381283436487706 1102 3 0.3410279707113695 1102 4 0.3120026580242268 1102 5 0.13123840161490108 1102 6 0.1801544406390576 1102 7 0.280207139380515 1102 8 0.3853450070685298 1102 9 0.24877847347588045 1102 10-11 0.3831507320753218 1102 12-13 0.2758417749369215 1102 14-15 0.4520777569458261 1102 16-17 0.21861648496274455 1102 18-19 0.37049282385772386 1102 20-21 0.2856295462711529 1102 22-23 0.2468534217785887 1102 24-25 0.28439773932303325 1102 26-27 0.33696496168852264 1102 28-29 0.3574271870899608 1102 30-31 0.3883134044197689 1102 32-33 0.26551625954502356 1102 34-35 0.3232556736174885 1102 36-37 0.2843993712757964 1102 38-39 0.3216529139627724 1102 40-41 0.4519094176472578 1102 42-43 0.41021174359894275 1102 44-45 0.33024154394221483 1102 46-47 0.3239211928401957 1102 48-49 0.37032489507991073 1102 50-51 0.2672116154983897 1102 52-53 0.26354233539245797 1102 54-55 0.2936665405049226 1102 56-57 0.1888552837311721 1102 58-59 0.08596306250789354 1102 60-61 0.29398191349608993 1102 62-63 0.32919206567171244 1102 64-65 0.2343593596836655 1102 66-67 0.31892060101552033 1102 68-69 0.12227898425554429 1102 70-71 0.26794321687248157 1102 72-73 0.1644251347434036 1102 74-75 0.33352836898853866 1102 76-77 0.27223815285532993 1102 78-79 0.2648561722283489 1102 80-81 0.1919503992811613 1102 82-83 0.10641639001302394 1102 84-85 0.11035311267441728 1102 86-87 0.20612397342543787 1102 88-89 0.2258777449127507 1102 90-91 0.27842293122864703 1102 92-93 0.20091019715844993 1102 94-95 0.2787342934030157 1102 96-97 0.20893093368092863 1102 98-99 0.33095849218461026 1102 100-101 0.3159914008020692 1102 102-103 0.25910298577334956 1102 104-105 0.30702802270192464 1102 106-107 0.24904228473621615 1102 108-109 0.21636309017513256 1102 110-111 0.23173451542617585 1102 112-113 0.26047340380915784 1102 114-115 0.39079441343337606 1102 116-117 0.3564268418698582 1102 118-119 0.35927568185391223 1102 120-121 0.5117313993262123 1102 122-123 0.5649690663601703 1102 124-125 0.4856968395137926 1103 1 0.0803432244601261 1103 2 0.15107864836870988 1103 3 0.22872546704226338 1103 4 0.12099639007040963 1103 5 -0.05309632819365362 1103 6 0.23942072984648632 1103 7 0.10285868839362422 1103 8 0.14075499984395634 1103 9 0.364821363607966 1103 10-11 0.24157837856575526 1103 12-13 0.06667800028022697 1103 14-15 0.3541517395999563 1103 16-17 0.1040484682628886 1103 18-19 0.22112151319352336 1103 20-21 0.14068044541708957 1103 22-23 -0.00438367437021725 1103 24-25 0.21983629980800146 1103 26-27 -0.013699196235535283 1103 28-29 -0.09500358201366765 1103 30-31 0.09059833908525405 1103 32-33 -0.012647004777228688 1103 34-35 0.15330913820984904 1103 36-37 0.22770369382919853 1103 38-39 0.08089971266442575 1103 40-41 0.12487216047795613 1103 42-43 0.23428995631267924 1103 44-45 0.17978241511961812 1103 46-47 0.2289459554244857 1103 48-49 0.27318263022804246 1103 50-51 0.31104562526016366 1103 52-53 0.37807557221449883 1103 54-55 0.4567559303005737 1103 56-57 0.5180123916706947 1103 58-59 0.46386778492659886 1103 60-61 0.38637896333599286 1103 62-63 0.48302380957611035 1103 64-65 0.27480043740533233 1103 66-67 0.2535957259648143 1103 68-69 0.3064794155260273 1103 70-71 0.24389826887356492 1103 72-73 0.15663409775896753 1103 74-75 0.253430654749657 1103 76-77 0.1736013863233481 1103 78-79 0.33046238549856355 1103 80-81 0.1759247957814125 1103 82-83 0.22370102508946843 1103 84-85 0.31740874463857693 1103 86-87 0.22057931709539602 1103 88-89 0.3210279147831834 1103 90-91 0.3802832070296347 1103 92-93 0.32345761315126964 1103 94-95 0.2599731319892591 1103 96-97 0.06154620831455304 1103 98-99 0.1553776165719185 1103 100-101 0.0037803578809452176 1103 102-103 0.029557514010132735 1103 104-105 0.18561560153344914 1103 106-107 0.23386035153975016 1103 108-109 0.1268135136976234 1103 110-111 0.24858106178431072 1103 112-113 0.20256835080944313 1103 114-115 0.16561846513073775 1103 116-117 0.16648325414295684 1103 118-119 0.18387903628704194 1103 120-121 0.1200052912083116 1103 122-123 0.06421045179766338 1103 124-125 0.38269261565259427 1104 1 0.28669460111485634 1104 2 0.3888716901297258 1104 3 0.30228714944514223 1104 4 0.2408816722108753 1104 5 0.22890067319249852 1104 6 0.35469426243182767 1104 7 0.22047615094125916 1104 8 0.16516016026172053 1104 9 0.24831996196478912 1104 10-11 0.27211951655722544 1104 12-13 0.19689071865973773 1104 14-15 0.28144242598078506 1104 16-17 0.1941106093486198 1104 18-19 0.21725099781181711 1104 20-21 0.26680812493989237 1104 22-23 0.4239062131885021 1104 24-25 0.36878698702401636 1104 26-27 0.3328074051603167 1104 28-29 0.4541761772081472 1104 30-31 0.4511607745093045 1104 32-33 0.48662119065264164 1104 34-35 0.399367564031877 1104 36-37 0.5598018613878608 1104 38-39 0.32349444313268094 1104 40-41 0.39300259344493327 1104 42-43 0.49180400305387195 1104 44-45 0.5429548300275471 1104 46-47 0.4474662351334615 1104 48-49 0.41921283459448944 1104 50-51 0.38553275587229763 1104 52-53 0.3017351877830379 1104 54-55 0.24462723776895956 1104 56-57 0.2659318170576199 1104 58-59 0.36334551961257944 1104 60-61 0.2720682606015501 1104 62-63 0.2575387575179633 1104 64-65 0.22391223612909528 1104 66-67 0.2824247174148553 1104 68-69 0.30633291990338307 1104 70-71 0.29708965075499094 1104 72-73 0.29097193080918515 1104 74-75 0.2711333878528208 1104 76-77 0.018457133599206088 1104 78-79 0.23478727305054292 1104 80-81 0.3111055680120387 1104 82-83 0.2496230045517862 1104 84-85 0.18441496682966374 1104 86-87 0.25803033367398953 1104 88-89 0.16144905433727175 1104 90-91 0.18057936392745688 1104 92-93 0.29983847752259507 1104 94-95 0.28740519506522233 1104 96-97 0.3925879235324494 1104 98-99 0.34458900537735815 1104 100-101 0.2976822334290432 1104 102-103 0.2470075337449451 1104 104-105 0.18819156709114893 1104 106-107 0.25853088793600065 1104 108-109 0.26958477981045803 1104 110-111 0.23226683776656998 1104 112-113 0.18897928214441606 1104 114-115 0.2600455022281416 1104 116-117 0.32090580544336333 1104 118-119 0.327128854767178 1104 120-121 0.38705300365390727 1104 122-123 0.3953589235957402 1104 124-125 0.5236978517765962 1105 1 0.06419686393653024 1105 2 0.03388100916733805 1105 3 0.17716911047889639 1105 4 0.1775331140821521 1105 5 0.1866515394646342 1105 6 0.17885832451346317 1105 7 0.16501840791160305 1105 8 0.1943972530354472 1105 9 0.20290157178772006 1105 10-11 0.21333447873904277 1105 12-13 0.22167400368906698 1105 14-15 0.21265819794582796 1105 16-17 0.25917603852719395 1105 18-19 0.19572415153871958 1105 20-21 0.22453613442741727 1105 22-23 0.26877721602666327 1105 24-25 0.3204135411221145 1105 26-27 0.3690096132563241 1105 28-29 0.24148889851080213 1105 30-31 0.17396544565242067 1105 32-33 0.31830611444812007 1105 34-35 0.3438546381936547 1105 36-37 0.11520246245599708 1105 38-39 0.2839156348984915 1105 40-41 0.29691169135947604 1105 42-43 0.3254062845528125 1105 44-45 0.36585001403772566 1105 46-47 0.19736991011259875 1105 48-49 0.2619072184865914 1105 50-51 0.41527335095847917 1105 52-53 0.32579130053620986 1105 54-55 0.3074817628245583 1105 56-57 0.2010959353016375 1105 58-59 0.2769912812463602 1105 60-61 0.28288245845248383 1105 62-63 0.21486807937675678 1105 64-65 0.13886344668971162 1105 66-67 0.029394234300795574 1105 68-69 0.18521841002468165 1105 70-71 0.24779899473914924 1105 72-73 0.15943799240234569 1105 74-75 0.14965349594582023 1105 76-77 0.08439079406754502 1105 78-79 0.1744809529536866 1105 80-81 0.14909276037604258 1105 82-83 0.058350247357452645 1105 84-85 0.21832072112314194 1105 86-87 0.22822639264187927 1105 88-89 0.08959156834848159 1105 90-91 0.24957583353381096 1105 92-93 0.08209821679851714 1105 94-95 0.06429685521226247 1105 96-97 0.2825801783477253 1105 98-99 0.4016535803652488 1105 100-101 0.18719645492042503 1105 102-103 0.09470624736510658 1105 104-105 0.09589097294535875 1105 106-107 0.18450625224049233 1105 108-109 0.26145070321802777 1105 110-111 0.2424136175068412 1105 112-113 0.25879533074186156 1105 114-115 0.2956246996807188 1105 116-117 0.27802517140922234 1105 118-119 0.2706536582967871 1105 120-121 0.36434576771833704 1105 122-123 0.3652803746227917 1105 124-125 0.33911919374413557 1106 1 0.06492917981250557 1106 2 0.13508035499000215 1106 3 0.11450548073879574 1106 4 0.16590923002533486 1106 5 0.08839884887150617 1106 6 0.057800733677410676 1106 7 0.08100829834994272 1106 8 0.06533822613976525 1106 9 0.056737782726990815 1106 10-11 0.1414277679553635 1106 12-13 0.28167576770695035 1106 14-15 0.13969369756351568 1106 16-17 0.13962966442248614 1106 18-19 0.20841762672228015 1106 20-21 0.20708012151779087 1106 22-23 0.2514221372390395 1106 24-25 0.21370008657259376 1106 26-27 0.32947294124997484 1106 28-29 0.18019486716470112 1106 30-31 0.306469276490553 1106 32-33 0.33307417404708417 1106 34-35 0.3583298335555227 1106 36-37 0.362220557925383 1106 38-39 0.19314946105949815 1106 40-41 0.28855341435773596 1106 42-43 0.3225006241051318 1106 44-45 0.17970233716853556 1106 46-47 0.3952070005762849 1106 48-49 0.37804823059806836 1106 50-51 0.5153670799370005 1106 52-53 0.4192817639697992 1106 54-55 0.5855667599145491 1106 56-57 0.6386764108535345 1106 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-1.2549617741008703 2216 84-85 -1.1397399753281192 2216 86-87 -1.2764266679534089 2216 88-89 -1.1760960720165166 2216 90-91 -1.1190728760475714 2216 92-93 -1.1848710746394495 2216 94-95 -1.1675278343829767 2216 96-97 -1.0382904772622048 2216 98-99 -1.1123561834024471 2216 100-101 -1.1512943136270195 2216 102-103 -1.1460834963432163 2216 104-105 -1.1583547822629008 2216 106-107 -1.3179655206049787 2216 108-109 -1.2556997198882094 2216 110-111 -1.3395279887264024 2216 112-113 -1.2959574967909226 2216 114-115 -1.2724415463365482 2216 116-117 -1.2214933362970655 2216 118-119 -1.2051883871807512 2216 120-121 -1.2651482005007608 2216 122-123 -1.298741817860435 2216 124-125 -1.4705179263008787 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15578.0 3 3282.0 4 3279.0 5 3233.0 6 4070.0 7 3593.0 8 3211.0 9 2919.0 10 2899.0 11 3115.0 12 3064.0 13 3101.0 14 2975.0 15 2876.0 16 2678.0 17 2449.0 18 2147.0 19 2170.0 20 2097.0 21 2196.0 22 2440.0 23 3770.0 24 6268.0 25 6418.0 26 9569.0 27 8203.0 28 8263.0 29 11097.0 30 12361.0 31 13585.0 32 17944.0 33 24401.0 34 35405.0 35 57011.0 36 124945.0 37 293816.0 38 168987.0 39 964.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.68070293971966 19.679048077735146 13.065648945567595 22.574600036977603 2 14.887425350608208 22.757208737559388 38.909208764948424 23.446157146883984 3 17.43064510607219 29.604697075169177 28.834061783199626 24.130596035559005 4 11.798070238920083 18.748908473895263 37.11086024242438 32.342161044760275 5 12.368921777105099 41.731927556132355 32.92242086796127 12.976729798801276 6 29.384473685111566 38.62724084288905 18.385554516952624 13.602730955046754 7 27.496208485916334 35.17041557733148 21.424650718533286 15.908725218218894 8 26.248010269576376 36.0801026957638 20.332506061902723 17.339380972757095 9 27.421372164148444 18.147167572008946 19.036837540512163 35.39462272333044 10-11 23.74744473991546 29.5999388392116 26.883475575906985 19.76914084496596 12-13 24.596422201657102 27.195101494952358 27.209136999072285 20.99933930431826 14-15 22.267395448707088 28.052973073523564 24.686321870365582 24.99330960740377 16-17 21.691483134348964 31.39980168125911 25.22379322817952 21.684921956212406 18-19 22.02340740199748 30.278932881739674 26.63693193229747 21.060727783965383 20-21 23.326323188592674 29.309955280373458 26.0955966173294 21.268124913704472 22-23 23.122139856557084 29.300363485328507 26.292980243080454 21.28451641503395 24-25 22.566710486075927 29.38507903469493 26.323711321383414 21.724499157845727 26-27 22.04423555401132 29.58985093735398 26.172255874045003 22.1936576345897 28-29 22.24862651705036 29.59825088822082 26.354611644807523 21.79851094992129 30-31 22.230995776902702 29.473761683122785 26.162835146302193 22.132407393672317 32-33 21.852754358769257 29.746663920476102 26.04586784851754 22.3547138722371 34-35 22.626151401650542 29.403767212188814 25.94213110217914 22.027950283981504 36-37 22.546556669361635 29.28361582952434 25.838773194176355 22.33105430693767 38-39 22.125890675358566 29.371276907110737 26.18084910337682 22.321983314153872 40-41 21.970085584693695 29.556190953322993 26.308092344052863 22.16563111793045 42-43 22.067314824324168 29.42350484406559 26.110654662056533 22.398525669553706 44-45 21.927567454077828 29.381120389240213 26.268522343200505 22.422789813481458 46-47 22.23777380904832 29.5133488151821 25.93515882208617 22.313718553683408 48-49 21.974738518909025 29.570641544398725 25.979569899835 22.47505003685725 50-51 22.04764934283163 29.53146619726748 26.115902418984422 22.304982040916467 52-53 22.368144034680427 29.317009463743997 25.886052802294074 22.428793699281506 54-55 21.86764176451022 29.369611567895028 26.18586157574001 22.57688509185474 56-57 21.99573203088001 29.625868001072696 25.99974894299294 22.378651025054346 58-59 22.05108657693458 28.974433886683755 26.194196097407325 22.78028343897434 60-61 21.979032649160594 29.327646949749674 26.280962501248055 22.412357899841677 62-63 21.843713216142305 29.428740805996906 26.42960293546239 22.297943042398398 64-65 21.771661090597057 29.13629496419296 26.329812726218787 22.762231218991193 66-67 21.855805031898665 29.49305600722983 26.06333633435231 22.587802626519192 68-69 21.9077840840368 29.190486297008828 26.370812594565358 22.53091702438902 70-71 22.059295918734133 29.12584079006451 26.213136893659776 22.601726397541576 72-73 21.901183853943802 29.364370275281697 25.965939238339754 22.768506632434747 74-75 21.944541123441645 29.519698741905113 26.16911533962856 22.366644795024676 76-77 22.100520611038963 29.077016654532013 26.542661118959966 22.279801615469065 78-79 22.093337715064255 29.042546150809134 26.37727844302495 22.486837691101663 80-81 22.181820878967894 29.36808064394657 26.168887631141125 22.28121084594441 82-83 22.451007912219325 28.903677230746055 26.34555653688071 22.29975832015391 84-85 22.15616759415732 28.872725156581797 26.395885798267642 22.575221450993237 86-87 22.386258670060997 28.9430698562868 26.384599541172125 22.286071932480073 88-89 22.339159999292516 28.766712177688113 26.352992459015834 22.54113536400354 90-91 22.182321103963904 29.071140764983173 26.315795479572262 22.43074265148066 92-93 22.517397844013978 28.670722549751325 26.435821413328036 22.376058192906655 94-95 21.955341239349643 28.42622883478495 26.819675049265214 22.798754876600192 96-97 22.26661607934982 28.626811087750927 26.556972063819895 22.54960076907936 98-99 21.959411657309783 29.05250125519193 26.309963941759097 22.678123145739193 100-101 21.598988565449424 29.0745784643402 26.618962800340952 22.707470169869428 102-103 21.775217743048938 28.866172891346984 26.58159673291289 22.777012632691182 104-105 21.292146948165545 29.533018604168166 26.43867631166632 22.736158135999972 106-107 21.50696217047647 29.400122549718787 26.292448814953346 22.800466464851393 108-109 21.654786342438605 29.492605368225387 26.129448560497227 22.72315972883878 110-111 21.555062236379 29.86593090983156 26.07178535709253 22.50722149669691 112-113 21.30781537422232 29.91410711106851 26.418082809554456 22.359994705154715 114-115 21.354869445403274 29.98799006113373 26.101790650505073 22.555349842957924 116-117 21.24876480240262 30.24362774966966 26.170670646684425 22.336936801243297 118-119 21.226500946837323 30.08186904945977 25.887998758470616 22.803631245232292 120-121 21.097785225211656 30.135691256320857 26.262588226702473 22.50393529176501 122-123 21.171376767357504 30.11065988573437 26.15991483136862 22.55804851553951 124-125 21.44103095550093 30.240980709552723 25.759042129514643 22.558946205431702 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3412.0 1 3263.0 2 3923.5 3 3632.5 4 1971.5 5 1125.5 6 762.0 7 667.5 8 749.0 9 804.5 10 632.5 11 470.0 12 426.5 13 402.0 14 425.0 15 461.5 16 485.5 17 517.0 18 549.5 19 640.0 20 796.5 21 1010.5 22 1279.0 23 1658.0 24 2104.5 25 2697.0 26 3592.5 27 4667.0 28 5654.5 29 7205.5 30 9732.5 31 11852.0 32 14685.0 33 17873.5 34 19665.0 35 21347.5 36 24563.5 37 26725.0 38 27435.5 39 28489.5 40 27868.5 41 28184.5 42 28241.5 43 28594.5 44 31283.0 45 33011.5 46 32437.5 47 31378.0 48 30320.5 49 28293.5 50 27179.0 51 25595.5 52 24762.5 53 26359.5 54 27321.0 55 26176.5 56 23818.5 57 21796.5 58 20685.0 59 18796.5 60 15730.0 61 12918.5 62 11337.5 63 9184.0 64 6629.0 65 5236.0 66 3888.5 67 3163.5 68 2563.0 69 1801.0 70 1385.0 71 986.0 72 742.0 73 649.5 74 484.0 75 304.5 76 188.5 77 117.5 78 85.5 79 54.0 80 30.5 81 18.5 82 14.0 83 10.5 84 9.5 85 7.0 86 4.5 87 5.0 88 7.5 89 6.0 90 4.0 91 4.0 92 2.0 93 1.5 94 5.5 95 6.0 96 3.0 97 3.5 98 5.0 99 6.0 100 14.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01974031783052766 2 0.013236282475960745 3 0.010155423623797467 4 0.03560103562499786 5 0.03194964735576731 6 0.023049388449517844 7 0.009128470673076374 8 4.5642353365381874E-4 9 0.011296482457932014 10-11 5.705294170672734E-5 12-13 0.003423176502403641 14-15 0.013977970718148196 16-17 0.0013692706009614563 18-19 0.0085579412560091 20-21 0.003423176502403641 22-23 2.852647085336367E-4 24-25 0.0062758235877400075 26-27 2.852647085336367E-4 28-29 0.005534135345552552 30-31 9.128470673076375E-4 32-33 0.0015404294260816381 34-35 1.7115882512018204E-4 36-37 0.00621877064603328 38-39 8.557941256009102E-4 40-41 0.012836911884013652 42-43 0.005591188287259279 44-45 5.705294170672734E-4 46-47 0.009584894206730192 48-49 0.002852647085336367 50-51 0.009299629498196555 52-53 0.003936652977764186 54-55 0.00256738237680273 56-57 0.009584894206730192 58-59 0.01608892956129711 60-61 0.0018827470763220023 62-63 0.006903405946514007 64-65 0.0017115882512018204 66-67 0.0011410588341345467 68-69 1.1410588341345469E-4 70-71 0.005191817695312188 72-73 4.5642353365381874E-4 74-75 0.0061617177043265525 76-77 0.011524694224758922 78-79 0.0014833764843749108 80-81 0.0037654941526440045 82-83 0.0014833764843749108 84-85 0.0 86-87 0.0017686411929085477 88-89 0.003936652977764186 90-91 0.005248870637018915 92-93 0.013806811893028015 94-95 0.0 96-97 7.416882421874554E-4 98-99 0.002168011784855639 100-101 0.0 102-103 2.2821176682690937E-4 104-105 0.007987411838941827 106-107 0.0 108-109 0.0 110-111 2.852647085336367E-4 112-113 0.0062758235877400075 114-115 0.0024532764933892755 116-117 1.1410588341345469E-4 118-119 0.004621288278244914 120-121 0.0016545353094950931 122-123 0.0 124-125 0.07833368896333663 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 876379.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.19065597548998 #Duplication Level Percentage of deduplicated Percentage of total 1 62.33167337804024 21.311608008451817 2 13.89629113158625 9.502466188306355 3 7.054660281582646 7.236103881346366 4 4.23599281984846 5.793254928723375 5 2.7091833367684255 4.631437772098963 6 1.9135806758518163 3.9255947141637035 7 1.4184369743378284 3.3948103428749725 8 1.0185582500467438 2.786013977467626 9 0.788419588630971 2.426092462729698 >10 4.293501341596519 25.830359099513156 >50 0.23805920543674286 5.649564070504743 >100 0.09796514397412179 6.054579288089451 >500 0.002674816217679471 0.5675846565541608 >1k 0.0010030560816298014 0.8905306091756118 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4375 0.4992132399338643 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1952 0.22273468442306354 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1463 0.1669369074338842 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 972 0.11091091867787796 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.705294170672734E-5 0.0 12-13 0.0 0.0 0.0 2.2821176682690937E-4 0.0 14-15 0.0 0.0 0.0 2.2821176682690937E-4 0.0 16-17 0.0 0.0 0.0 2.852647085336367E-4 0.0 18-19 0.0 0.0 0.0 6.275823587740008E-4 0.0 20-21 0.0 0.0 0.0 9.128470673076374E-4 0.0 22-23 0.0 0.0 0.0 0.002510329435096003 0.0 24-25 0.0 0.0 0.0 0.005191817695312188 0.0 26-27 0.0 0.0 0.0 0.008272676547475464 0.0 28-29 0.0 0.0 0.0 0.013806811893028015 0.0 30-31 0.0 0.0 0.0 0.029895741454325123 0.0 32-33 0.0 0.0 0.0 0.05157585930288151 0.0 34-35 0.0 0.0 0.0 0.07605157129506754 0.0 36-37 0.0 0.0 0.0 0.11427704223857486 0.0 38-39 0.0 0.0 0.0 0.17326978396333093 0.0 40-41 0.0 0.0 0.0 0.24726744935695627 0.0 42-43 0.0 0.0 0.0 0.3133347558533466 0.0 44-45 0.0 0.0 0.0 0.38322460944408754 0.0 46-47 0.0 0.0 0.0 0.46218588076619815 0.0 48-49 0.0 0.0 0.0 0.5621996875780912 0.0 50-51 0.0 0.0 0.0 0.6717983885967145 0.0 52-53 0.0 0.0 0.0 0.77420841896029 0.0 54-55 0.0 0.0 0.0 0.873594643413409 0.0 56-57 0.0 0.0 0.0 0.9904390680287867 0.0 58-59 0.0 0.0 0.0 1.1237717927974082 0.0 60-61 0.0 0.0 0.0 1.2747909294951156 0.0 62-63 0.0 0.0 0.0 1.4244978485335682 0.0 64-65 0.0 0.0 0.0 1.5811652264602416 0.0 66-67 0.0 0.0 0.0 1.7475316044770586 0.0 68-69 0.0 0.0 0.0 1.940541706270917 0.0 70-71 0.0 0.0 0.0 2.168239996622466 0.0 72-73 0.0 0.0 0.0 2.405694340005865 0.0 74-75 0.0 0.0 0.0 2.635960012734216 0.0 76-77 0.0 0.0 0.0 2.8729008796422555 0.0 78-79 0.0 0.0 0.0 3.148751852794282 0.0 80-81 0.0 0.0 0.0 3.4727555087467863 0.0 82-83 0.0 0.0 0.0 3.8179258060724868 0.0 84-85 0.0 0.0 0.0 4.155108691559246 0.0 86-87 0.0 0.0 0.0 4.505870177172206 0.0 88-89 0.0 0.0 0.0 4.897253357280355 0.0 90-91 0.0 0.0 0.0 5.333993626045352 0.0 92-93 0.0 0.0 0.0 5.826588724741237 0.0 94-95 0.0 0.0 0.0 6.30577638213604 0.0 96-97 0.0 0.0 0.0 6.783765927755001 0.0 98-99 0.0 0.0 0.0 7.291023632469514 0.0 100-101 0.0 0.0 0.0 7.875873337905175 0.0 102-103 0.0 0.0 0.0 8.51754777328074 0.0 104-105 0.0 0.0 0.0 9.142106326144283 0.0 106-107 0.0 0.0 0.0 9.757308196567923 0.0 108-109 0.0 0.0 0.0 10.396129984858149 0.0 110-111 0.0 0.0 0.0 11.07825495590378 0.0 112-113 0.0 0.0 0.0 11.838143086495684 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2295 0.0 58.618732 1 GGTATCA 1370 0.0 57.354042 1 TATACCG 35 0.007781903 51.022755 5 TCAACGC 2795 0.0 46.215603 4 ATCAACG 2880 0.0 44.8516 3 CAACGCA 2945 0.0 43.861668 5 AACGCAG 3005 0.0 42.579987 6 TATCAAC 3205 0.0 41.603577 2 GTACATG 6140 0.0 40.524586 1 TACATGG 6480 0.0 37.847126 2 ACGCAGA 3725 0.0 34.34192 7 GAACAAA 1290 0.0 34.147015 1 CGCAGAG 3775 0.0 34.04856 8 ACATGGG 7005 0.0 33.566006 3 ATGGGAT 1855 0.0 32.089783 5 GTGTAAG 230 7.2759576E-12 31.057327 1 CATGGGA 4615 0.0 30.8274 4 GTATTAC 175 1.663102E-8 30.613651 1 CATGGGG 2465 0.0 30.185877 4 GCAGAGT 4210 0.0 28.551651 9 >>END_MODULE