FastQCFastQC Report
Fri 27 May 2016
SRR940158_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940158_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876379
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88021.0043599858052281No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71130.8116351487199032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50280.5737243818028501No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17970.20504827249397806No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17230.19660443712138242No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13280.15153261317306782No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12630.14411573075119324No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9950.11353535399638741No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28950.086.81741
GTATCAA57750.062.8070221
ATCAACG77350.046.7354473
TCAACGC77650.046.554894
TATCAAC79650.045.38592
CAACGCA80250.045.0770075
AACGCAG81300.044.4948356
GTGGTAT7450.039.9720651
ACGCAGA92100.039.143537
CGCAGAG92950.038.7855768
GTACATG58200.035.714561
GCAGAGT100400.035.6112949
TACATGG62950.033.0177732
ACATGGG62200.032.458423
GAGTACT68700.030.6544512-13
CATGGGG22800.030.5611064
CAGAGTA98150.030.30586810-11
TGGTATC10150.029.3374272
GTACTTT74000.028.25794614-15
AGTACTT70900.028.19291712-13
Produced by FastQC (version 0.11.5)