##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940158_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 876379 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.72684192569653 34.0 31.0 34.0 31.0 34.0 2 32.793493454316 34.0 31.0 34.0 31.0 34.0 3 32.88836793213895 34.0 31.0 34.0 31.0 34.0 4 36.30649981343688 37.0 37.0 37.0 35.0 37.0 5 36.27321056300984 37.0 37.0 37.0 35.0 37.0 6 36.32682777656699 37.0 37.0 37.0 35.0 37.0 7 36.30323980834776 37.0 37.0 37.0 35.0 37.0 8 36.28879172138995 37.0 37.0 37.0 35.0 37.0 9 38.10427337943972 39.0 39.0 39.0 35.0 39.0 10-11 38.08156630864044 39.0 39.0 39.0 35.0 39.0 12-13 38.07916380926517 39.0 39.0 39.0 35.0 39.0 14-15 39.54675716784633 41.0 39.5 41.0 36.5 41.0 16-17 39.54122303250078 41.0 39.5 41.0 36.5 41.0 18-19 39.60442399920582 41.0 40.0 41.0 37.0 41.0 20-21 39.591589369439475 41.0 40.0 41.0 37.0 41.0 22-23 39.53877203812506 41.0 40.0 41.0 36.0 41.0 24-25 39.496357169672024 41.0 39.5 41.0 36.0 41.0 26-27 39.36141783406494 41.0 39.0 41.0 35.5 41.0 28-29 39.23025654425768 41.0 39.0 41.0 35.0 41.0 30-31 38.997835981921064 41.0 38.5 41.0 35.0 41.0 32-33 38.72893291601008 41.0 38.0 41.0 35.0 41.0 34-35 38.559426914611144 40.5 38.0 41.0 35.0 41.0 36-37 38.400434058780505 40.0 38.0 41.0 35.0 41.0 38-39 38.20598222914972 40.0 38.0 41.0 34.0 41.0 40-41 38.060407084149666 40.0 38.0 41.0 34.0 41.0 42-43 37.84255670206612 40.0 37.0 41.0 33.5 41.0 44-45 37.79258631254286 40.0 38.0 41.0 33.0 41.0 46-47 37.420053424374615 40.0 37.0 41.0 33.0 41.0 48-49 37.04071126761367 40.0 37.0 41.0 31.5 41.0 50-51 36.82266747605773 40.0 37.0 41.0 31.0 41.0 52-53 36.69127797448364 40.0 36.0 41.0 31.0 41.0 54-55 36.51243069493906 40.0 36.0 41.0 31.0 41.0 56-57 36.327252250453284 40.0 35.0 41.0 30.5 41.0 58-59 36.10783918829639 39.5 35.0 41.0 30.0 41.0 60-61 35.86286983143138 39.0 35.0 41.0 30.0 41.0 62-63 35.60396757567217 39.0 35.0 41.0 29.0 41.0 64-65 35.30738584562158 38.0 35.0 41.0 29.0 41.0 66-67 34.98531457280469 37.0 35.0 40.5 29.0 41.0 68-69 34.64884427855985 37.0 35.0 40.0 28.0 41.0 70-71 34.367295998648984 36.0 35.0 39.0 28.5 41.0 72-73 34.16377617446334 36.0 35.0 39.0 29.0 41.0 74-75 33.85340760104931 35.5 35.0 39.0 29.0 41.0 76-77 33.51381137612836 35.0 35.0 37.0 28.0 39.5 78-79 33.188676360341816 35.0 35.0 37.0 28.0 39.0 80-81 32.89554804485274 35.0 34.0 36.5 27.0 39.0 82-83 32.63125999139642 35.0 34.0 36.0 27.0 37.5 84-85 32.40390344816569 35.0 34.0 36.0 27.0 37.0 86-87 32.19813003278262 35.0 34.0 35.5 26.0 37.0 88-89 32.02599845500634 35.0 34.0 35.0 26.0 36.0 90-91 31.89734749463417 35.0 34.0 35.0 25.5 36.0 92-93 31.760994387131596 35.0 34.0 35.0 25.0 36.0 94-95 31.652198421002787 35.0 34.0 35.0 25.0 36.0 96-97 31.573231444386504 35.0 34.0 35.0 24.5 35.5 98-99 31.497098287384794 35.0 34.0 35.0 24.0 35.0 100-101 31.447420008923082 35.0 34.0 35.0 24.0 35.0 102-103 30.6883249142209 34.5 32.0 35.0 21.5 35.0 104-105 31.270503400925854 35.0 33.5 35.0 23.0 35.0 106-107 31.311599775896045 35.0 34.0 35.0 23.0 35.0 108-109 31.291770455476453 35.0 34.0 35.0 23.0 35.0 110-111 31.23253181557294 35.0 34.0 35.0 20.0 35.0 112-113 31.15631193809984 35.0 34.0 35.0 19.5 35.0 114-115 31.070541968714448 35.0 34.0 35.0 18.5 35.0 116-117 30.979092949511568 35.0 34.0 35.0 16.5 35.0 118-119 30.878861200462357 35.0 34.0 35.0 7.5 35.0 120-121 30.751509906102267 35.0 34.0 35.0 2.0 35.0 122-123 30.579087358323285 35.0 33.0 35.0 2.0 35.0 124-125 28.917467785056466 33.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15565471776886142 1101 2 0.13664200303385599 1101 3 0.11130830463208241 1101 4 0.019546846714121102 1101 5 0.08451983417385378 1101 6 0.12941092935786003 1101 7 0.15083965075250205 1101 8 0.15344401505161187 1101 9 0.1548080154221978 1101 10-11 0.16982172091389458 1101 12-13 0.16971643942239467 1101 14-15 0.21543612858454253 1101 16-17 0.2822863998041427 1101 18-19 0.24330820518354557 1101 20-21 0.2078220539346205 1101 22-23 0.1542452863822703 1101 24-25 0.2097032849236129 1101 26-27 0.22200831554035005 1101 28-29 0.14980408024902658 1101 30-31 0.02491251190182453 1101 32-33 0.21486947205720242 1101 34-35 0.21514768355502412 1101 36-37 0.18433127943542615 1101 38-39 0.22439481702037511 1101 40-41 0.27491602879401 1101 42-43 0.28029705128022897 1101 44-45 0.13828307373405835 1101 46-47 0.16299020366469819 1101 48-49 0.16617572740409514 1101 50-51 0.12676441161895724 1101 52-53 -0.015059885506296666 1101 54-55 -0.0447118770844952 1101 56-57 -0.00925512804020201 1101 58-59 0.08938955151630523 1101 60-61 0.032995889496653774 1101 62-63 0.10263354815800341 1101 64-65 0.06656915835215926 1101 66-67 0.08992897540706934 1101 68-69 0.11337088577595011 1101 70-71 0.08911045744545731 1101 72-73 0.0669844125861303 1101 74-75 0.13300031323544914 1101 76-77 0.20481714877892898 1101 78-79 0.1314307699258208 1101 80-81 0.10963831445214822 1101 82-83 0.09382337055505019 1101 84-85 0.07705965078739752 1101 86-87 0.05281719457989453 1101 88-89 0.13278861812302978 1101 90-91 0.17616679694955906 1101 92-93 0.04196588009733304 1101 94-95 0.09767094599753179 1101 96-97 0.07314529194149699 1101 98-99 0.06500416842283485 1101 100-101 0.03547274233980602 1101 102-103 -0.0016205284278001386 1101 104-105 0.007822496077597663 1101 106-107 0.024145393400299753 1101 108-109 0.044399586862784446 1101 110-111 0.09424444537213361 1101 112-113 0.140948572182662 1101 114-115 0.12372027772915928 1101 116-117 0.19526328164707252 1101 118-119 0.20323618688696143 1101 120-121 0.1404332240643491 1101 122-123 0.08228379884601011 1101 124-125 -0.026592386813728552 1102 1 0.13162198682005766 1102 2 0.10541258166435341 1102 3 0.0813109234949394 1102 4 -0.11419965427088385 1102 5 -0.08819382599448744 1102 6 0.08657973821749465 1102 7 0.16637938871419777 1102 8 0.2256258180315882 1102 9 0.24824268515871495 1102 10-11 0.1652525307644055 1102 12-13 0.179019276017101 1102 14-15 0.24732130215848258 1102 16-17 0.26202856273609854 1102 18-19 0.34081420472887203 1102 20-21 0.3003926371256611 1102 22-23 0.2315404363726259 1102 24-25 0.2487271055197624 1102 26-27 0.29275769036457433 1102 28-29 0.3151400098727848 1102 30-31 0.39888612841323123 1102 32-33 0.4156326045488612 1102 34-35 0.3576494093096443 1102 36-37 0.44698180358804507 1102 38-39 0.5223001413275057 1102 40-41 0.5787887672608178 1102 42-43 0.6248288448972517 1102 44-45 0.5390969251001181 1102 46-47 0.48935545004164993 1102 48-49 0.3724056497434134 1102 50-51 0.28670990610380187 1102 52-53 0.24603901422580776 1102 54-55 0.3830589869260379 1102 56-57 0.3195140824244689 1102 58-59 0.3309938157713219 1102 60-61 0.254634261158202 1102 62-63 0.22791296838737196 1102 64-65 0.2730181254215083 1102 66-67 0.2665004485968865 1102 68-69 0.32955697843947007 1102 70-71 0.4203188726326488 1102 72-73 0.23818514393295942 1102 74-75 0.30723484864296324 1102 76-77 0.3251366224082233 1102 78-79 0.27415914133171526 1102 80-81 0.2361926164699213 1102 82-83 0.254745237921739 1102 84-85 0.40858868665199566 1102 86-87 0.3131708585348818 1102 88-89 0.3062758447749019 1102 90-91 0.4299632843565604 1102 92-93 0.3622013289973758 1102 94-95 0.4584476357686924 1102 96-97 0.4103504884840419 1102 98-99 0.47590167829193675 1102 100-101 0.43460229866021294 1102 102-103 0.2319968950948379 1102 104-105 0.2949700983669956 1102 106-107 0.3250078215629557 1102 108-109 0.44278252542038743 1102 110-111 0.4008621528928309 1102 112-113 0.3681566916249217 1102 114-115 0.300634828863668 1102 116-117 0.24485860169077256 1102 118-119 0.21351021391721758 1102 120-121 0.2833222513053606 1102 122-123 0.2770083449155365 1102 124-125 0.2952675862513665 1103 1 -0.007509921204039927 1103 2 0.0435140411106687 1103 3 -0.09349480850440983 1103 4 -0.04099730907405785 1103 5 0.014777339765032593 1103 6 0.022208038431884347 1103 7 0.024649664866423393 1103 8 0.09913105808505662 1103 9 0.029280282550985248 1103 10-11 -0.0919543075794067 1103 12-13 -0.037201025927338094 1103 14-15 0.011790247859863712 1103 16-17 0.09041569239067115 1103 18-19 -0.015531470066179054 1103 20-21 0.07590953153739832 1103 22-23 0.170893971420071 1103 24-25 0.20937728950875822 1103 26-27 0.08046062750803884 1103 28-29 0.19388427033206312 1103 30-31 0.27289171660984124 1103 32-33 0.16213825989473207 1103 34-35 0.2637192900043317 1103 36-37 -0.01546970258727498 1103 38-39 0.27984961312102286 1103 40-41 0.32122345512691197 1103 42-43 0.2013979511370394 1103 44-45 0.20906102262426884 1103 46-47 0.2336706617960047 1103 48-49 0.2381122769974766 1103 50-51 0.3155444909388123 1103 52-53 0.23987092342071037 1103 54-55 0.15722979675999937 1103 56-57 0.3802187434101896 1103 58-59 0.3640866973289576 1103 60-61 0.3995341192149837 1103 62-63 0.3368573198941718 1103 64-65 0.1379843889400334 1103 66-67 0.1532199760864259 1103 68-69 0.36679486385044413 1103 70-71 0.38943296371736125 1103 72-73 0.2342966847228638 1103 74-75 0.22676282027359917 1103 76-77 0.14835015914988503 1103 78-79 0.18856069671539188 1103 80-81 0.1607371550304748 1103 82-83 0.10829681441439476 1103 84-85 0.05145960371817182 1103 86-87 -0.017027579607557186 1103 88-89 -0.1556140180270873 1103 90-91 -0.073132124768442 1103 92-93 -0.23898469927143395 1103 94-95 -0.07907193221701192 1103 96-97 0.1411432148752212 1103 98-99 0.1592791005726859 1103 100-101 0.05526781334386399 1103 102-103 0.0357009865170177 1103 104-105 0.05394554515067895 1103 106-107 -0.010014000227709374 1103 108-109 0.07096393726304129 1103 110-111 -0.024514692248558845 1103 112-113 0.004935964746138666 1103 114-115 0.021895113893371843 1103 116-117 0.1467641946394771 1103 118-119 0.247902347741924 1103 120-121 0.0775414223872346 1103 122-123 0.26416871596518376 1103 124-125 0.2833126058761728 1104 1 -0.03199927450837947 1104 2 -0.0019757122782664283 1104 3 0.058226161451528924 1104 4 0.023408902070926274 1104 5 -0.12667613928709898 1104 6 0.021207153396261447 1104 7 -0.030747907414848896 1104 8 0.020147353786640565 1104 9 0.11825263091286331 1104 10-11 0.17120739799334928 1104 12-13 0.10722378847786018 1104 14-15 0.10630316170158238 1104 16-17 0.11594196528607625 1104 18-19 0.18587711060339984 1104 20-21 0.13774146098241857 1104 22-23 0.21747318076130995 1104 24-25 0.1605595596992231 1104 26-27 0.11436746033329825 1104 28-29 0.14720234566919999 1104 30-31 0.10693810756388444 1104 32-33 0.07390445153390601 1104 34-35 0.20078881177526853 1104 36-37 0.03858886602588285 1104 38-39 -0.08169013333041875 1104 40-41 -0.005469865885068259 1104 42-43 -0.21081008477089114 1104 44-45 -0.11782829942766426 1104 46-47 -0.17463486543331186 1104 48-49 -0.05462605285812572 1104 50-51 -0.22801926597679056 1104 52-53 -0.13757151221888364 1104 54-55 -0.14402346487242568 1104 56-57 -0.34685620711806564 1104 58-59 -0.1932355243394568 1104 60-61 -0.3664376081401599 1104 62-63 -0.1604670301704303 1104 64-65 -0.09101790514774422 1104 66-67 -0.2850174694070944 1104 68-69 -0.20819675053805042 1104 70-71 -0.25191308521149836 1104 72-73 -0.26148457811655135 1104 74-75 -0.21102873969261537 1104 76-77 -0.23660800708862695 1104 78-79 -0.2468313659833825 1104 80-81 -0.18364610176591611 1104 82-83 -0.26475748797681575 1104 84-85 -0.2324899990934668 1104 86-87 -0.1157306050284248 1104 88-89 0.012813851569582368 1104 90-91 -0.16837212837863547 1104 92-93 -0.26133589002826696 1104 94-95 -0.18978355743607978 1104 96-97 -0.05092399664290426 1104 98-99 -0.026416730060862648 1104 100-101 -0.034375383938254345 1104 102-103 -0.0500033307594947 1104 104-105 -0.01782080813883269 1104 106-107 -0.015000913285415862 1104 108-109 -0.031310213634856865 1104 110-111 -0.007922968476663073 1104 112-113 0.13421649948546843 1104 114-115 0.0647082070322611 1104 116-117 -0.04615504889080313 1104 118-119 0.003037103508461314 1104 120-121 -0.024244072996975063 1104 122-123 -0.1677182284796359 1104 124-125 -0.10823921046748453 1105 1 -0.10032604032559789 1105 2 -0.15292453006005502 1105 3 -0.06417126709489906 1105 4 -0.09082081775714101 1105 5 -0.2511972896494825 1105 6 0.042830977741218135 1105 7 -0.11582869635269333 1105 8 0.02644929582967137 1105 9 -0.17557460052971408 1105 10-11 0.0788584780743875 1105 12-13 0.08780368469499678 1105 14-15 0.04393156785227603 1105 16-17 0.1031575126015909 1105 18-19 0.17303127112595007 1105 20-21 0.09446442499800156 1105 22-23 0.11276918918304801 1105 24-25 0.16254940606245327 1105 26-27 0.0559958037910917 1105 28-29 0.09255961027371029 1105 30-31 -0.07442329477168386 1105 32-33 0.009101875378370039 1105 34-35 0.06311586754713261 1105 36-37 0.08759838359099348 1105 38-39 0.3149756495405782 1105 40-41 0.2661666929541724 1105 42-43 0.17401882240052657 1105 44-45 0.11804849336056833 1105 46-47 -0.033777318057737205 1105 48-49 0.01862504394100739 1105 50-51 -0.19574711873207917 1105 52-53 -0.1833363459455697 1105 54-55 -0.005048663497888128 1105 56-57 -0.17270187549616622 1105 58-59 -0.08622169141341374 1105 60-61 0.06099177419115165 1105 62-63 0.029793171465591683 1105 64-65 0.07664150786390422 1105 66-67 0.03210409081007981 1105 68-69 0.04244421900756379 1105 70-71 0.08350663718958629 1105 72-73 0.07876119109261737 1105 74-75 0.023702601661057088 1105 76-77 -0.008758517056143944 1105 78-79 0.03229993253014385 1105 80-81 0.03417579033177276 1105 82-83 0.025998771190387515 1105 84-85 0.06423547263791107 1105 86-87 0.09696353070430774 1105 88-89 0.12123239717278977 1105 90-91 0.030281737617912086 1105 92-93 0.09126364473820914 1105 94-95 0.1394050789542689 1105 96-97 0.23017298534725583 1105 98-99 0.07061097366818814 1105 100-101 0.13531599722540477 1105 102-103 0.11932560710531703 1105 104-105 0.15191987837448906 1105 106-107 0.3437180357726852 1105 108-109 0.22616819976470381 1105 110-111 0.2128738974010318 1105 112-113 0.2628714626076807 1105 114-115 0.2802391962608368 1105 116-117 0.2803792617551011 1105 118-119 0.3691309573843853 1105 120-121 0.26297069065734036 1105 122-123 0.38593284898918867 1105 124-125 0.7457029971322022 1106 1 -0.05741257844670855 1106 2 -0.05610293003372391 1106 3 1.0606633119181197E-4 1106 4 -0.12478126773077491 1106 5 -0.15744470806520638 1106 6 -0.007412475361057602 1106 7 0.030413355473413617 1106 8 -0.07878741487326124 1106 9 0.14488657834355934 1106 10-11 -0.022746778202012763 1106 12-13 -0.005287079615435175 1106 14-15 -0.0707243151163226 1106 16-17 -0.06665560709510743 1106 18-19 -0.12001557907726124 1106 20-21 0.04642226745504985 1106 22-23 -0.20470589251147686 1106 24-25 -0.09971719542666335 1106 26-27 -0.10886222693139302 1106 28-29 -0.16686561803927447 1106 30-31 -0.13493407733101748 1106 32-33 -0.011723784373636192 1106 34-35 0.08505801397178914 1106 36-37 -0.039803149738069976 1106 38-39 0.023613622758567487 1106 40-41 0.01103318350890703 1106 42-43 0.1323454526729364 1106 44-45 0.14281990086670504 1106 46-47 0.012235908349616409 1106 48-49 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-0.8834072067616319 2216 72-73 -0.8849712870680122 2216 74-75 -0.7630420768735888 2216 76-77 -0.7977540937822667 2216 78-79 -0.8623455070465482 2216 80-81 -0.9358315031082718 2216 82-83 -0.8142031135424261 2216 84-85 -0.8315976800454834 2216 86-87 -0.8530672520446387 2216 88-89 -0.9231371538230064 2216 90-91 -0.9367171322360903 2216 92-93 -0.8145584858904833 2216 94-95 -0.947938201205357 2216 96-97 -0.9159949718825473 2216 98-99 -0.9920868380107564 2216 100-101 -1.0302337711091205 2216 102-103 -1.0885126249725232 2216 104-105 -1.0467757809207008 2216 106-107 -0.8837963463708114 2216 108-109 -0.9323468192300375 2216 110-111 -0.8987427123729503 2216 112-113 -1.0773316264580366 2216 114-115 -1.1281862203660857 2216 116-117 -1.1487859717554123 2216 118-119 -1.1036564181237196 2216 120-121 -1.1068888552224436 2216 122-123 -1.139963680508778 2216 124-125 -1.5798113252763883 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 14.0 5 74.0 6 204.0 7 403.0 8 806.0 9 1356.0 10 2404.0 11 3370.0 12 3955.0 13 6068.0 14 10417.0 15 10934.0 16 4036.0 17 3180.0 18 3097.0 19 3068.0 20 2963.0 21 3062.0 22 3092.0 23 2864.0 24 3445.0 25 4059.0 26 5082.0 27 6062.0 28 7240.0 29 9032.0 30 11183.0 31 14093.0 32 17878.0 33 23801.0 34 33933.0 35 61031.0 36 121601.0 37 307056.0 38 184343.0 39 1167.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.885129559521594 19.187683542180032 14.097180769287837 21.830006129010535 2 15.52670646000906 21.82319843078366 39.671550879146835 22.978544230060443 3 17.344862723350552 28.600851924246523 31.001552998793887 23.05273235360904 4 11.772371056785037 17.954518979737742 38.25583759127839 32.01727237219883 5 11.68004400028299 39.6174850917091 34.25860207814615 14.443868829861769 6 28.817971813753463 38.82251430084504 17.893861022721566 14.465652862679931 7 26.022759559505648 35.07671909071304 22.320480066272697 16.58004128350862 8 26.638474906404646 33.591174594553266 20.85581694677759 18.914533552264487 9 28.102453390599276 14.883971432451029 20.258814964758397 36.7547602121913 10-11 24.106180088751554 27.479264108336686 27.637757180397976 20.776798622513777 12-13 25.997770371038104 25.347480941464823 28.51876870623326 20.135979981263812 14-15 22.88051174206593 25.396717630157728 27.793169393607105 23.929601234169233 16-17 20.672562898015585 29.106242846987435 28.053501966614903 22.16769228838208 18-19 20.871164188096703 26.110963407384247 32.43054660141332 20.58732580310573 20-21 21.811796038015515 25.216373281422765 32.678612791954166 20.293217888607554 22-23 21.58826261240856 25.309312523463024 32.84988572295776 20.252539141170658 24-25 21.25476610634429 25.41459348528961 32.85756423795643 20.473076170409662 26-27 20.756031351732528 25.670799962116845 32.67758583900344 20.895582847147182 28-29 20.946070846626775 25.56551327518451 32.82510166915419 20.66331420903452 30-31 21.087871790614464 25.354195658760275 32.64722277852808 20.91070977209718 32-33 20.727105510287217 25.50825613119438 32.59685592648843 21.167782432029977 34-35 21.399816745951238 25.29607624098706 32.334811765229425 20.969295247832274 36-37 21.537697903294823 25.146712309228352 32.02943945228926 21.28615033518756 38-39 21.17355685927941 25.218955051955287 32.206403968148464 21.401084120616833 40-41 21.102868224174827 25.38281119402176 32.16173148930513 21.352589092498274 42-43 21.32969868059367 25.271372317228046 31.77369608354376 21.625232918634516 44-45 21.3356809775207 25.24073147450275 31.569157843506996 21.85442970446956 46-47 21.818981615400546 25.58028478071474 30.613784199046396 21.986949404838317 48-49 21.881571785722844 25.933186441026084 29.969739119718753 22.21550265353232 50-51 21.96947955956632 25.997203215332448 29.715123918328413 22.31819330677282 52-53 22.172338876603476 25.99260821243801 29.4248600489743 22.410192861984214 54-55 21.72291204405528 26.11086736117332 29.496715651845975 22.66950494292543 56-57 21.88306053391436 26.276548948996236 29.315368451801255 22.52502206528815 58-59 21.840935050589998 25.65877682547906 29.657765824279775 22.84252229965117 60-61 22.025953427603973 25.98543435905189 29.766909807702728 22.221702405641405 62-63 21.912119875642087 26.05479713102712 29.971135681694864 22.061947311635933 64-65 21.80630614182429 25.855021726429563 29.939396498210762 22.399275633535385 66-67 21.793861663495466 26.168403456273126 29.789160493052364 22.24857438717904 68-69 21.86014296415855 26.046257674381984 29.84920242339596 22.244396938063506 70-71 22.044997849040286 25.999169284523475 29.563087020869443 22.392745845566797 72-73 21.762249859360967 26.400542244770108 29.129053570633882 22.708154325235036 74-75 21.652789489478867 26.53486676426523 29.273236807363023 22.539106938892875 76-77 21.676633997555808 26.235304320241724 29.66293647723603 22.425125204966434 78-79 21.695601247641562 26.349952389021347 29.31563996401078 22.638806399326313 80-81 21.69243373570963 26.767133322759495 29.030143420012163 22.510289521518708 82-83 21.965018140200154 26.2826653399797 29.09603665581317 22.65627986400698 84-85 21.568065871044375 26.35166976844493 29.09899712339068 22.98126723712001 86-87 21.827542943670434 26.392948491558464 29.020946503771604 22.758562060999495 88-89 21.732033229111988 26.373895976447496 28.903621881917978 22.990448912522538 90-91 21.65194911303846 26.570961202600145 28.790604500906902 22.98648518345449 92-93 21.867097707707618 26.189737176034544 28.893975771628888 23.04918934462895 94-95 21.430429796484262 25.948855758862095 29.14907941507055 23.471635029583098 96-97 21.79051953046104 26.044418376696953 28.98257429403126 23.182487798810747 98-99 21.596054481386133 26.31878640156287 28.688539555604244 23.39661956144675 100-101 21.42985925521888 26.110473356170793 28.839614395358872 23.620052993251463 102-103 21.606742867966812 25.817783270174573 28.8637813326213 23.711692529237315 104-105 21.225528875256813 26.27622410538081 28.775526621628327 23.722720397734047 106-107 21.500971611597265 26.014315724133052 28.561615465455013 23.92309719881467 108-109 21.68279010056344 25.991241708865275 28.41058412381428 23.915384066757007 110-111 21.4868276540815 26.389193969390522 28.30997446832789 23.81400390820009 112-113 21.28194316941512 26.433816040892733 28.466813451148898 23.81742733854325 114-115 21.345102974854484 26.439816563381825 28.1527170322429 24.06236342952079 116-117 21.185493017853027 26.61790916704892 28.275470173829103 23.92112764126895 118-119 20.983782395983397 26.637550100557704 27.99475103053816 24.38391647292074 120-121 20.84942701730644 26.754463536894423 28.2171868563715 24.178922589427636 122-123 20.799395683518913 26.93852540388561 27.99334065146595 24.26873826112953 124-125 20.91149127172935 27.074305596778096 27.52224875685499 24.491954374637558 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1171.0 1 846.0 2 1005.0 3 1345.5 4 724.0 5 420.0 6 1078.0 7 2828.5 8 4750.0 9 4977.5 10 3093.0 11 1345.0 12 1050.0 13 1108.0 14 1219.0 15 1290.0 16 1369.0 17 1470.5 18 1551.5 19 1690.0 20 1778.5 21 1854.5 22 2086.5 23 2471.5 24 2887.0 25 3485.0 26 4255.5 27 5168.0 28 6170.0 29 7740.0 30 10233.5 31 12253.0 32 14841.0 33 17571.5 34 19187.5 35 20758.5 36 23719.0 37 26033.5 38 26963.5 39 28149.5 40 27714.0 41 27911.0 42 27770.5 43 27986.5 44 30949.0 45 32776.5 46 31952.5 47 31132.0 48 30166.0 49 28042.5 50 26998.0 51 25223.0 52 24080.0 53 25611.0 54 26567.5 55 25194.0 56 22890.0 57 21125.0 58 20001.5 59 18146.5 60 14945.5 61 12030.5 62 10515.0 63 8683.0 64 6411.0 65 5001.0 66 3685.5 67 2972.5 68 2392.0 69 1664.0 70 1270.5 71 917.0 72 672.5 73 573.0 74 416.5 75 251.0 76 159.5 77 108.5 78 66.0 79 23.5 80 11.0 81 4.0 82 3.0 83 4.0 84 3.0 85 2.5 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02487508258413312 2 0.0020539059014421843 3 0.0011410588341345467 4 0.08683457727763902 5 0.002396223551682548 6 0.02361991786658512 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.699000090143649E-4 26-27 0.0 28-29 7.987411838941827E-4 30-31 0.007131617713340917 32-33 0.0 34-35 0.0 36-37 4.5642353365381874E-4 38-39 5.705294170672734E-5 40-41 5.705294170672734E-5 42-43 0.0 44-45 0.00450718239483146 46-47 0.002339170609975821 48-49 0.0 50-51 0.0017686411929085477 52-53 1.1410588341345469E-4 54-55 0.0029097000270430946 56-57 2.852647085336367E-4 58-59 0.0022821176682690934 60-61 1.7115882512018204E-4 62-63 0.00427897062800455 64-65 0.0030808588521632763 66-67 1.7115882512018204E-4 68-69 6.275823587740008E-4 70-71 0.0029667529687498217 72-73 0.002510329435096003 74-75 0.0 76-77 0.001825694134615275 78-79 6.275823587740008E-4 80-81 2.2821176682690937E-4 82-83 0.001198111775841274 84-85 0.0 86-87 0.0031379117938700038 88-89 0.004450129453124733 90-91 0.0050777118118987335 92-93 0.005819400054086189 94-95 0.0038796000360574595 96-97 0.003423176502403641 98-99 0.004906552986778551 100-101 0.004678341219951642 102-103 0.001825694134615275 104-105 0.0016545353094950931 106-107 0.0 108-109 0.007588041246994737 110-111 0.0018827470763220023 112-113 0.00427897062800455 114-115 0.0 116-117 1.1410588341345469E-4 118-119 0.0018827470763220023 120-121 0.0 122-123 0.0019398000180287298 124-125 0.042789706280045504 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 876379.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.93543386289121 #Duplication Level Percentage of deduplicated Percentage of total 1 58.39737682197108 18.065481884424223 2 15.388918612549732 9.521257479198955 3 7.698698984403256 7.144877797869439 4 4.663283886113223 5.770428409709676 5 3.020668408891727 4.672284389249741 6 2.0996938782818475 3.897296466234338 7 1.531094261407182 3.31555456831299 8 1.1292138209827602 2.7946175580859878 9 0.8775334260788222 2.443218953844396 >10 4.7962320229181055 26.067728669677425 >50 0.27211874588552926 5.769317933872288 >100 0.11778263904088894 6.496788990345384 >500 0.004799919459338296 0.9510059096250041 >1k 0.0014768982951810143 0.6980058988371846 >5k 0.0011076737213857608 2.3921350907130026 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8802 1.0043599858052281 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7113 0.8116351487199032 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5028 0.5737243818028501 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1797 0.20504827249397806 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1723 0.19660443712138242 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1328 0.15153261317306782 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1263 0.14411573075119324 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 995 0.11353535399638741 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.705294170672734E-5 0.0 12-13 0.0 0.0 0.0 2.2821176682690937E-4 0.0 14-15 0.0 0.0 0.0 2.2821176682690937E-4 0.0 16-17 0.0 0.0 0.0 2.852647085336367E-4 0.0 18-19 0.0 0.0 0.0 5.134764753605461E-4 0.0 20-21 0.0 0.0 0.0 7.987411838941828E-4 0.0 22-23 0.0 0.0 0.0 0.0023962235516825484 0.0 24-25 0.0 0.0 0.0 0.005020658870192006 0.0 26-27 0.0 0.0 0.0 0.008044464780648556 0.0 28-29 0.0 0.0 0.0 0.013578600126201107 0.0 30-31 0.0 0.0 0.0 0.029724582629204944 0.0 32-33 0.0 0.0 0.0 0.05134764753605461 0.0 34-35 0.0 0.0 0.0 0.07570925364482718 0.0 36-37 0.0 0.0 0.0 0.1137065128215076 0.0 38-39 0.0 0.0 0.0 0.17269925454626367 0.0 40-41 0.0 0.0 0.0 0.24669691993988901 0.0 42-43 0.0 0.0 0.0 0.312821279377986 0.0 44-45 0.0 0.0 0.0 0.382711132968727 0.0 46-47 0.0 0.0 0.0 0.4618435631159578 0.0 48-49 0.0 0.0 0.0 0.5619144228695576 0.0 50-51 0.0 0.0 0.0 0.6732817650810894 0.0 52-53 0.0 0.0 0.0 0.776946960162213 0.0 54-55 0.0 0.0 0.0 0.8784441434584809 0.0 56-57 0.0 0.0 0.0 0.9981982681009015 0.0 58-59 0.0 0.0 0.0 1.1346118517216865 0.0 60-61 0.0 0.0 0.0 1.2899099590473986 0.0 62-63 0.0 0.0 0.0 1.4428688957631344 0.0 64-65 0.0 0.0 0.0 1.6011908089993028 0.0 66-67 0.0 0.0 0.0 1.769782251742682 0.0 68-69 0.0 0.0 0.0 1.963933412370675 0.0 70-71 0.0 0.0 0.0 2.1944843498075604 0.0 72-73 0.0 0.0 0.0 2.433250910850214 0.0 74-75 0.0 0.0 0.0 2.667510289498037 0.0 76-77 0.0 0.0 0.0 2.909642974101388 0.0 78-79 0.0 0.0 0.0 3.189544706114592 0.0 80-81 0.0 0.0 0.0 3.519481868004596 0.0 82-83 0.0 0.0 0.0 3.87087093597633 0.0 84-85 0.0 0.0 0.0 4.214843121526189 0.0 86-87 0.0 0.0 0.0 4.571937483668595 0.0 88-89 0.0 0.0 0.0 4.968056057938403 0.0 90-91 0.0 0.0 0.0 5.409246456156525 0.0 92-93 0.0 0.0 0.0 5.90863085491551 0.0 94-95 0.0 0.0 0.0 6.395064235907068 0.0 96-97 0.0 0.0 0.0 6.87653401097014 0.0 98-99 0.0 0.0 0.0 7.387386051012176 0.0 100-101 0.0 0.0 0.0 7.975830091775362 0.0 102-103 0.0 0.0 0.0 8.621840550720636 0.0 104-105 0.0 0.0 0.0 9.253701880122641 0.0 106-107 0.0 0.0 0.0 9.87940149182032 0.0 108-109 0.0 0.0 0.0 10.523186886039031 0.0 110-111 0.0 0.0 0.0 11.210617780663387 0.0 112-113 0.0 0.0 0.0 11.977523423085216 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2895 0.0 86.8174 1 GTATCAA 5775 0.0 62.807022 1 ATCAACG 7735 0.0 46.735447 3 TCAACGC 7765 0.0 46.55489 4 TATCAAC 7965 0.0 45.3859 2 CAACGCA 8025 0.0 45.077007 5 AACGCAG 8130 0.0 44.494835 6 GTGGTAT 745 0.0 39.972065 1 ACGCAGA 9210 0.0 39.14353 7 CGCAGAG 9295 0.0 38.785576 8 GTACATG 5820 0.0 35.71456 1 GCAGAGT 10040 0.0 35.611294 9 TACATGG 6295 0.0 33.017773 2 ACATGGG 6220 0.0 32.45842 3 GAGTACT 6870 0.0 30.65445 12-13 CATGGGG 2280 0.0 30.561106 4 CAGAGTA 9815 0.0 30.305868 10-11 TGGTATC 1015 0.0 29.337427 2 GTACTTT 7400 0.0 28.257946 14-15 AGTACTT 7090 0.0 28.192917 12-13 >>END_MODULE