FastQCFastQC Report
Fri 27 May 2016
SRR940157_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940157_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2441190
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41100.057.2121161
GGTATCA24900.050.6838071
ATCAACG50050.046.1487923
TCAACGC51450.044.8930444
CAACGCA52000.044.4182175
TATCAAC53450.043.658732
AACGCAG53500.043.2663766
ACGCAGA62650.036.750617
GTACATG102300.036.4858631
CGCAGAG65700.034.6865778
TACATGG109700.033.4277272
ACATGGG108850.032.704353
CATGGGG69050.027.8465614
GCAGAGT79400.027.2028359
TAAGGTG12800.025.1140375
CATGGGA49650.023.739894
CCTATAC7400.023.3291553
AGAGTAC74950.021.86990410-11
ATGGGGA37550.021.5606445
GAGTACA84050.021.5312181
Produced by FastQC (version 0.11.5)