FastQCFastQC Report
Fri 27 May 2016
SRR940157_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940157_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2441190
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37590.1539822791343566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28340.1160909228695841No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35450.068.5705641
GTATCAA67400.061.7005921
ATCAACG86650.047.5641673
TCAACGC87150.047.2219734
CAACGCA87650.046.9227685
TATCAAC89600.046.0655752
AACGCAG89300.046.0520026
ACGCAGA106450.038.6750687
CGCAGAG111150.036.8791128
GTACATG97050.034.5626221
TACATGG104600.031.7157362
GCAGAGT128350.031.2880579
ACATGGG102300.031.089093
CATGGGG63250.028.9075534
GAGTACT72950.026.13806312-13
TGGTATC15950.025.392152
CAGAGTA124100.025.36031210-11
GTGGTAT17400.024.9958211
AGAGTAC120600.024.9370210-11
GTACTTT77750.024.21831914-15
Produced by FastQC (version 0.11.5)