FastQCFastQC Report
Fri 27 May 2016
SRR940156_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940156_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050250
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20200.068.3599851
GTATCAA41150.056.844451
TCAACGC49600.045.360264
ATCAACG50550.044.6255383
CAACGCA52000.043.265655
AACGCAG53800.041.80796
TATCAAC57250.040.4426422
GTACATG113550.038.0552751
TACATGG120100.035.1373182
ACGCAGA65800.034.0862777
ACATGGG117900.034.076533
CGCAGAG66150.033.8192868
CATGGGG54700.032.861324
ATGGGGA33250.027.924655
CATGGGA74900.027.1775134
GCAGAGT78400.027.0930929
GAGTACA86100.023.9187431
AGAGTAC67950.023.37912810-11
AGTACAT85700.022.8496992
GGTAAGG6800.022.7577763
Produced by FastQC (version 0.11.5)