FastQCFastQC Report
Fri 27 May 2016
SRR940156_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940156_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050250
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55910.27269845140836485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43320.2112913059383002No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26090.12725277405194488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34150.081.647931
GTATCAA76150.065.6420441
TCAACGC101350.049.0686724
ATCAACG101500.048.937483
CAACGCA104400.047.5956425
TATCAAC104950.047.4434282
AACGCAG105900.046.9180536
ACGCAGA123500.040.2229237
CGCAGAG125000.039.645068
GCAGAGT141000.034.5556379
GTACATG114500.033.873931
ACATGGG117450.031.2877063
TACATGG122500.030.9710242
CATGGGG55850.030.0723614
GAGTACT73000.029.29747212-13
CAGAGTA133550.027.97498710-11
AGAGTAC131650.027.97202710-11
AGTACTT80000.026.73394412-13
GTACTTT79750.026.33286714-15
GTGGTAT16850.024.750741
Produced by FastQC (version 0.11.5)