FastQCFastQC Report
Fri 27 May 2016
SRR940155_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940155_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371673
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19400.075.7828751
GTATCAA38450.058.2059481
TCAACGC47650.045.5938034
ATCAACG48300.044.9802253
TATCAAC50450.043.7712142
CAACGCA50550.042.976575
AACGCAG51700.041.7781686
GTACATG104050.040.0434841
TACATGG108350.038.1796342
ACATGGG113600.035.1576653
ACGCAGA62550.034.242157
CGCAGAG62950.034.0270468
CATGGGG45200.032.9213334
CATGGGA68800.030.885574
GGACCGA2151.2187229E-1030.4430076
GCAGAGT68400.030.184989
ATGGGAG28150.028.33265
ATGGGGA25300.027.289625
CAGAGTA68000.024.5875310-11
TGGTATC9550.023.6840572
Produced by FastQC (version 0.11.5)