FastQCFastQC Report
Fri 27 May 2016
SRR940155_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940155_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371673
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64130.4675312556272523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49220.358831879026561No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29430.21455550995025785No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31650.082.468171
GTATCAA66450.064.837951
TCAACGC87400.049.075454
ATCAACG87750.048.8797073
TATCAAC88350.048.8174672
CAACGCA91650.046.94165
AACGCAG94400.045.8262676
GCGTATC558.4645563E-443.3394361
ACGCAGA109150.039.570367
CGCAGAG109100.039.479438
GTACATG100800.038.2498471
TACATGG106450.035.9279632
GCAGAGT121550.035.3867269
GTGGTAT12400.035.0822261
ACATGGG107550.034.5080873
TGGTATC12450.033.015892
CATGGGG41400.030.9372254
CATGGGA65000.030.8858664
GAGTACT69800.028.97881312-13
TAAGGTG7200.028.9259535
Produced by FastQC (version 0.11.5)