FastQCFastQC Report
Fri 27 May 2016
SRR940154_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940154_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247875
Sequences flagged as poor quality0
Sequence length125
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50202.025214321734745No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18820.7592536560766515No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15990.6450832072617246No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11360.4582955118507312No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8210.3312153303076147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7760.3130610186585981No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6150.24810892586989408No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT4510.18194654563792234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.17871911245587493No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3310.1335350479072113No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3140.12667675239536055No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2940.11860816944024206No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT2750.11094301563287948No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT2650.10690872415532021No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT2640.10650529500756428No Hit
GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG2610.10529500756429652No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7150.081.54831
GTATCAA15750.065.3522341
GCCAATC150.004235985759.49697582-83
GATAGTC507.43706E-659.4969757
GAACAAA7000.057.7970621
CGCAAAG453.166866E-452.88622
CGTCACT350.00779064850.9974061
GATTGGC1152.728484E-1046.5628479
CATATAG653.513208E-545.7669073
ATCAACG20750.044.443523
GTACATG32200.044.345571
TCAACGC20950.044.019244
GTCACTA558.517576E-443.2705271
GGTTAAC558.517576E-443.2705271
CAACGCA21400.043.09365
AACGCAG21250.042.8378226
TCCGCCA355.4491033E-542.49783736-37
CGACCGT355.4491033E-542.49783718-19
TACATGG32700.042.2119182
TATCAAC24450.041.611382