FastQCFastQC Report
Fri 27 May 2016
SRR940154_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940154_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247875
Sequences flagged as poor quality0
Sequence length125
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92363.726071608673727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73812.9777105395864853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49812.0094805849722643No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22940.9254664649520927No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21090.8508320726172466No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17900.7221381744831064No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15140.6107917297024711No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10530.4248108925869894No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8460.34130105900151286No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7390.29813414019162887No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT6870.2771558245083207No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6870.2771558245083207No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6240.25173978819969745No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT4450.1795259707513868No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4400.17750882501260715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4210.16984367120524457No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3980.1605648008068583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3830.15451336359051943No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3570.14402420574886535No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT3310.1335350479072113RNA PCR Primer, Index 39 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3170.12788703983862834No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2970.11981845688350982No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC2760.1113464447806354No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG2610.10529500756429652No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT2610.10529500756429652No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT2580.10408472012102875No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT2540.10247100353000504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20400.098.9674761
GTATCAA45900.065.5241851
TAGATAG657.456038E-964.059225
AGCCGGT150.00423299959.507572102-103
GACGCTG150.004236383459.49556468-69
GATAGTC803.8387952E-852.048117
GTGGTAT3800.051.719381
AGATAGT856.188566E-848.9864586
ATCAACG62150.048.4779933
TATCAAC62400.048.1883432
TCAACGC62700.048.0527424
GTATAGA1004.090907E-947.644521
CAACGCA63800.047.1766175
AACGCAG65000.046.397176
TGGTATC4250.046.2338682
CGACCGT502.2269523E-741.63849318-19
ACGCAGA73300.041.062327
CGCAGAG73800.040.703528
GCAGAGT77000.038.625699
GTTCGAC554.70307E-737.85317216-17
Produced by FastQC (version 0.11.5)