##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940154_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 247875 Sequences flagged as poor quality 0 Sequence length 125 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.558854261220375 33.0 31.0 34.0 31.0 34.0 2 32.59333535047907 34.0 31.0 34.0 31.0 34.0 3 32.677938477054965 34.0 31.0 34.0 31.0 34.0 4 36.191475542107916 37.0 35.0 37.0 35.0 37.0 5 36.22448814926879 37.0 37.0 37.0 35.0 37.0 6 36.32702370146243 37.0 37.0 37.0 35.0 37.0 7 36.29428542612204 37.0 37.0 37.0 35.0 37.0 8 36.3021482602118 37.0 37.0 37.0 35.0 37.0 9 38.0676671709531 39.0 39.0 39.0 35.0 39.0 10-11 38.05830559757942 39.0 39.0 39.0 35.0 39.0 12-13 38.04883308119011 39.0 39.0 39.0 35.0 39.0 14-15 39.24178315683308 41.0 38.5 41.0 36.0 41.0 16-17 39.2797034795764 41.0 39.0 41.0 36.0 41.0 18-19 39.52356026222895 41.0 39.5 41.0 36.0 41.0 20-21 39.52454866364095 41.0 39.5 41.0 36.0 41.0 22-23 39.388116994452844 41.0 39.0 41.0 35.0 41.0 24-25 39.28517599596571 41.0 39.0 41.0 35.0 41.0 26-27 39.034844175491685 41.0 38.5 41.0 35.0 41.0 28-29 38.800494200706 41.0 38.0 41.0 35.0 41.0 30-31 38.16011296016137 40.0 36.5 41.0 35.0 41.0 32-33 37.406936964195665 40.0 35.0 41.0 33.5 41.0 34-35 36.97107816439738 40.0 35.0 41.0 33.0 41.0 36-37 36.627931417044884 39.5 35.0 41.0 33.0 41.0 38-39 36.32580937972769 39.0 35.0 41.0 33.0 41.0 40-41 36.0051134644478 39.0 35.0 41.0 31.5 41.0 42-43 35.530507312153304 39.0 35.0 41.0 29.5 41.0 44-45 34.97156631366616 39.0 35.0 41.0 23.5 41.0 46-47 33.75618154311649 39.0 35.0 41.0 6.0 41.0 48-49 32.683064044377204 39.0 33.0 41.0 2.0 41.0 50-51 32.34110943015633 39.0 33.0 41.0 2.0 41.0 52-53 32.285595562279376 39.0 33.0 41.0 2.0 41.0 54-55 32.15612708018154 38.0 33.0 41.0 2.0 41.0 56-57 32.00247705496722 38.0 33.0 41.0 2.0 41.0 58-59 31.813167927382754 37.5 33.0 41.0 2.0 41.0 60-61 31.57765002521432 37.0 33.0 41.0 2.0 41.0 62-63 31.339479576399395 36.5 33.0 41.0 2.0 41.0 64-65 31.078279374684822 36.0 33.0 40.0 2.0 41.0 66-67 30.773054967221384 35.5 32.5 40.0 2.0 41.0 68-69 30.44138981341402 35.0 32.0 39.0 2.0 41.0 70-71 30.159923348461923 35.0 31.5 39.0 2.0 41.0 72-73 29.92898033282905 35.0 32.0 39.0 2.0 41.0 74-75 29.63072718103883 35.0 31.0 37.5 2.0 40.5 76-77 29.33317599596571 35.0 31.0 37.0 2.0 39.5 78-79 29.022713061018656 35.0 31.0 37.0 2.0 39.0 80-81 28.719570347957642 35.0 31.0 36.0 2.0 39.0 82-83 28.43056379223399 35.0 30.0 36.0 2.0 37.5 84-85 28.164024205748866 35.0 30.0 35.5 2.0 37.0 86-87 27.94573272818961 35.0 29.5 35.0 2.0 37.0 88-89 27.73602824004034 35.0 29.0 35.0 2.0 36.0 90-91 27.561607665153808 35.0 29.0 35.0 2.0 36.0 92-93 27.388518406454864 35.0 29.0 35.0 2.0 36.0 94-95 27.223372667675243 35.0 28.0 35.0 2.0 36.0 96-97 27.0880988401412 35.0 27.0 35.0 2.0 35.0 98-99 26.976274331820473 35.0 26.5 35.0 2.0 35.0 100-101 26.880393343419065 35.0 25.5 35.0 2.0 35.0 102-103 26.16220272314675 34.0 24.0 35.0 2.0 35.0 104-105 26.602557740796772 35.0 25.0 35.0 2.0 35.0 106-107 26.574237014624305 35.0 24.0 35.0 2.0 35.0 108-109 26.49973373676248 35.0 24.0 35.0 2.0 35.0 110-111 26.39065658093797 35.0 21.5 35.0 2.0 35.0 112-113 26.242719112455873 35.0 18.5 35.0 2.0 35.0 114-115 26.08028240040343 35.0 11.5 35.0 2.0 35.0 116-117 25.9049621785174 35.0 2.0 35.0 2.0 35.0 118-119 25.739818456883512 35.0 2.0 35.0 2.0 35.0 120-121 25.536169440242055 35.0 2.0 35.0 2.0 35.0 122-123 25.309910237014623 35.0 2.0 35.0 2.0 35.0 124-125 23.94499041855774 33.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.058489987464284354 1101 2 0.04146831141366647 1101 3 6.0016559572773076E-5 1101 4 -0.060007359612583855 1101 5 0.2130203536207702 1101 6 0.05104750632578714 1101 7 0.10437633214947084 1101 8 0.22897616700316092 1101 9 0.2841014124056329 1101 10-11 0.29045847103610356 1101 12-13 0.15224163609284602 1101 14-15 0.33531831468900464 1101 16-17 0.1857749546772638 1101 18-19 0.2932236897989213 1101 20-21 0.2405220699565902 1101 22-23 0.20445305499537625 1101 24-25 0.0874872929733499 1101 26-27 0.24512443217870583 1101 28-29 0.35918809764100956 1101 30-31 0.3521161184671868 1101 32-33 0.21447845730971693 1101 34-35 -0.041901989117285154 1101 36-37 -0.15547779689389074 1101 38-39 -0.08343033847371117 1101 40-41 -0.11886410155668159 1101 42-43 0.08049114522431466 1101 44-45 0.15991081223508274 1101 46-47 -0.15251735452979176 1101 48-49 -0.011084049531781659 1101 50-51 0.059371345869642767 1101 52-53 -0.033827282376428514 1101 54-55 0.10363737788718197 1101 56-57 0.015154587145097764 1101 58-59 -0.04066156123737841 1101 60-61 0.21619949468503208 1101 62-63 0.3163933037018971 1101 64-65 0.29378750526152686 1101 66-67 0.3432285230171921 1101 68-69 0.43461966581335787 1101 70-71 0.473584198542607 1101 72-73 0.5303801113414224 1101 74-75 0.6473200905876517 1101 76-77 0.6071169697082155 1101 78-79 0.49265898355269755 1101 80-81 0.5810479053364936 1101 82-83 0.4974021835752751 1101 84-85 0.471179762965523 1101 86-87 0.4493039414841853 1101 88-89 0.5962625940689072 1101 90-91 0.6348635180850302 1101 92-93 0.6356832879478311 1101 94-95 0.48614430569876177 1101 96-97 0.5267242824467928 1101 98-99 0.6025897917465777 1101 100-101 0.49628847750695115 1101 102-103 0.39370883616910035 1101 104-105 0.4801717659352285 1101 106-107 0.23457220366910647 1101 108-109 0.253880775314272 1101 110-111 0.3570225284197228 1101 112-113 0.40350534347056666 1101 114-115 0.4902129121954566 1101 116-117 0.37141205750803863 1101 118-119 0.4082393253272514 1101 120-121 0.2715159399888698 1101 122-123 0.1703136218401582 1101 124-125 -7.715367794780548E-4 1102 1 0.17720972926095158 1102 2 0.2540019316395927 1102 3 0.3095095413937159 1102 4 -0.2791395095139606 1102 5 -0.04864362342782158 1102 6 0.057090181599235734 1102 7 0.1955723156531448 1102 8 0.13661468233398466 1102 9 0.25838866361596047 1102 10-11 0.276795211218996 1102 12-13 0.30593537824812245 1102 14-15 0.4532401364569836 1102 16-17 0.2395673161619385 1102 18-19 0.2295514645882406 1102 20-21 0.2832063087946679 1102 22-23 0.2398215326715416 1102 24-25 0.05200226518063289 1102 26-27 0.36895444831641555 1102 28-29 0.404418508256029 1102 30-31 0.272091015365163 1102 32-33 0.47492314083132214 1102 34-35 0.7353617727618627 1102 36-37 0.7212659721575747 1102 38-39 0.9828149546946534 1102 40-41 1.0509494182568346 1102 42-43 0.9300877025131769 1102 44-45 0.819333440888478 1102 46-47 0.5047804758449601 1102 48-49 1.0123245931076283 1102 50-51 0.9102409890469829 1102 52-53 0.7113762320578516 1102 54-55 0.7438113065226517 1102 56-57 0.764652525104573 1102 58-59 0.6673086736559384 1102 60-61 0.6782489836575927 1102 62-63 0.690169527478119 1102 64-65 0.537780691562407 1102 66-67 0.4603255285757335 1102 68-69 0.590214071407761 1102 70-71 0.642779106199054 1102 72-73 0.6813304430609861 1102 74-75 0.7491042039102247 1102 76-77 0.7989046691882216 1102 78-79 0.5995264750355744 1102 80-81 0.8784927681659696 1102 82-83 0.8033462395193318 1102 84-85 0.7913698293094349 1102 86-87 0.6597396596122103 1102 88-89 0.510974804934964 1102 90-91 0.43392215175904525 1102 92-93 0.4198504028841761 1102 94-95 0.5671376713075098 1102 96-97 0.5995860360777776 1102 98-99 0.7492277691537872 1102 100-101 0.6347769121492313 1102 102-103 0.49805705205577766 1102 104-105 0.38737994237417084 1102 106-107 0.3892880006925985 1102 108-109 0.4511642610592972 1102 110-111 0.39923160139802505 1102 112-113 0.46406657710872423 1102 114-115 0.45792852991107225 1102 116-117 0.4314174367441872 1102 118-119 0.5220549968352302 1102 120-121 0.4715159399888691 1102 122-123 0.632856207459664 1102 124-125 0.7699295569985374 1103 1 -0.07984068770849717 1103 2 -0.022114889011639605 1103 3 -0.15815369954887615 1103 4 0.020109599451743065 1103 5 -0.09739963574658361 1103 6 -0.08180735810802275 1103 7 -0.09376295546350377 1103 8 0.12568004791756948 1103 9 0.012065261422115725 1103 10-11 0.009969369494370994 1103 12-13 -0.018305944981051425 1103 14-15 0.14682814988304926 1103 16-17 0.057917432082902565 1103 18-19 0.0014639875129063284 1103 20-21 -0.09897819585947332 1103 22-23 -0.2160679444729965 1103 24-25 -0.08500074530416413 1103 26-27 -0.1677942281083702 1103 28-29 -0.10665453925426505 1103 30-31 -0.2782134934413705 1103 32-33 -0.6815874650719991 1103 34-35 -0.9773618508929331 1103 36-37 -1.0253342562240277 1103 38-39 -0.9347860003556931 1103 40-41 -1.0184387958682208 1103 42-43 -1.2053408590287518 1103 44-45 -1.2748047645857525 1103 46-47 -1.573569986108744 1103 48-49 -1.6189256231628306 1103 50-51 -1.9804213176072274 1103 52-53 -1.810674703960693 1103 54-55 -1.9933854822935757 1103 56-57 -1.6858820954705251 1103 58-59 -1.7798694293926118 1103 60-61 -1.9402066722474487 1103 62-63 -1.9694121189456268 1103 64-65 -1.8525442331754753 1103 66-67 -1.7222153897951422 1103 68-69 -1.5430517855423034 1103 70-71 -1.3136034675924257 1103 72-73 -1.4527139875421504 1103 74-75 -1.4335145718259632 1103 76-77 -1.494531089834581 1103 78-79 -1.2012538287811658 1103 80-81 -1.3456931898256528 1103 82-83 -1.3524117451860214 1103 84-85 -1.206199290729323 1103 86-87 -1.212418546554094 1103 88-89 -1.6712546839746842 1103 90-91 -1.5891928349186948 1103 92-93 -1.719367217102672 1103 94-95 -1.6905702078578564 1103 96-97 -1.4098520067610778 1103 98-99 -1.1990316861906898 1103 100-101 -1.3400751588566848 1103 102-103 -1.4084708554842749 1103 104-105 -1.3854582181158115 1103 106-107 -1.6142847872931476 1103 108-109 -1.5625732385081648 1103 110-111 -1.695901448188465 1103 112-113 -1.7165106054927506 1103 114-115 -1.571536157448353 1103 116-117 -1.6120010206418804 1103 118-119 -1.8623614189577005 1103 120-121 -1.8296536506544072 1103 122-123 -1.7638703228701047 1103 124-125 -1.6287088041904276 1104 1 0.18803544200974187 1104 2 0.16590012959548517 1104 3 0.1879388044383674 1104 4 -0.09959069294591671 1104 5 -0.07807813122771279 1104 6 0.0819187184469996 1104 7 -0.19884336482022746 1104 8 0.034089803366804006 1104 9 -0.0948758603216362 1104 10-11 0.022844834672469005 1104 12-13 -0.047379576028369286 1104 14-15 0.09876528438597632 1104 16-17 0.05367268194999042 1104 18-19 0.01642444737468196 1104 20-21 -0.06828474822523134 1104 22-23 -0.11656967227735038 1104 24-25 -0.13050513126907504 1104 26-27 -0.02163693145765677 1104 28-29 0.025854764307666755 1104 30-31 -0.041728578502514324 1104 32-33 -0.07150639117513435 1104 34-35 -0.16983759517788855 1104 36-37 -0.15992855446964427 1104 38-39 -0.07007806574643638 1104 40-41 -0.05077698034456546 1104 42-43 -0.02263385477569102 1104 44-45 0.026198691022955245 1104 46-47 0.5913273424398966 1104 48-49 0.7989538292560923 1104 50-51 0.9049205882938836 1104 52-53 0.9893734751993293 1104 54-55 1.0075199536447599 1104 56-57 0.813544738660255 1104 58-59 0.9563081357323213 1104 60-61 0.8173358583213961 1104 62-63 0.933722849156446 1104 64-65 1.1777837173827415 1104 66-67 0.9212588260474952 1104 68-69 0.6405855749042679 1104 70-71 0.6007622288456353 1104 72-73 0.724792990129302 1104 74-75 0.9491193330118897 1104 76-77 1.0581586363748805 1104 78-79 0.9367877714314865 1104 80-81 0.9108774507910411 1104 82-83 0.8198453653934585 1104 84-85 0.8315964296321887 1104 86-87 0.7933380323932759 1104 88-89 0.5056375940689115 1104 90-91 0.5698066999032072 1104 92-93 0.3846416212811654 1104 94-95 0.39675036630482197 1104 96-97 0.6278606460831568 1104 98-99 0.5892375190193029 1104 100-101 0.39003847750695186 1104 102-103 0.4245800482903128 1104 104-105 0.517103584117045 1104 106-107 0.6278487188206228 1104 108-109 0.5237671389506353 1104 110-111 0.7369467708439608 1104 112-113 0.8911947374099611 1104 114-115 0.6432432152257554 1104 116-117 0.5202756938716746 1104 118-119 0.7950764465393725 1104 120-121 0.6703795763525058 1104 122-123 0.49824922790076087 1104 124-125 0.3837928571599143 1105 1 -0.050600921626624995 1105 2 -0.18339799874676288 1105 3 -0.04922697096270667 1105 4 -0.11392892824003553 1105 5 -0.2437532649175509 1105 6 0.02840936015822848 1105 7 0.022112517532711706 1105 8 -0.001237736740151263 1105 9 -0.04049751807564661 1105 10-11 0.07495044964572628 1105 12-13 0.07741988921227261 1105 14-15 0.13442704909185466 1105 16-17 0.11291399211042119 1105 18-19 0.25601335111799983 1105 20-21 0.2284899843950896 1105 22-23 0.37597711916649956 1105 24-25 0.4382448687309264 1105 26-27 0.396060261055716 1105 28-29 0.4547317696552682 1105 30-31 0.3372319829948154 1105 32-33 0.18885457139170825 1105 34-35 0.062197832629600214 1105 36-37 0.179593504353889 1105 38-39 0.43675682730168575 1105 40-41 0.5182571105645337 1105 42-43 0.8171221612670934 1105 44-45 0.7763992257823134 1105 46-47 0.10434539056824121 1105 48-49 -0.15074536860486631 1105 50-51 -0.39432740635851715 1105 52-53 -0.3828023269397107 1105 54-55 -0.3909259019702134 1105 56-57 -0.47664576936113434 1105 58-59 -0.47230148993612886 1105 60-61 -0.16588606681229123 1105 62-63 -0.31141418292911993 1105 64-65 -0.24823566764399985 1105 66-67 -0.2189818156637351 1105 68-69 -0.3922519919406504 1105 70-71 -0.3985191882666612 1105 72-73 -0.22799778527176429 1105 74-75 -0.2927422980041534 1105 76-77 -0.1648527272614828 1105 78-79 -0.021601266001670894 1105 80-81 -0.1408772283533466 1105 82-83 -0.10366733514130289 1105 84-85 -0.20961346341594123 1105 86-87 -0.15096343819496028 1105 88-89 -0.1387641438990066 1105 90-91 -0.02917391507005007 1105 92-93 0.1093909528319692 1105 94-95 0.03605517913904421 1105 96-97 -0.11471956782058612 1105 98-99 -0.12107330985769948 1105 100-101 -0.05625162944492246 1105 102-103 -0.0894908073246583 1105 104-105 -0.15498866187225957 1105 106-107 -0.04853831326493818 1105 108-109 -0.08221548137021983 1105 110-111 -0.06699707942341604 1105 112-113 -0.019400182376134723 1105 114-115 -0.1951257687314616 1105 116-117 -0.15439008152939593 1105 118-119 -0.1749603181665087 1105 120-121 0.03181005763592992 1105 122-123 -0.20637978279442848 1105 124-125 -0.22247385407002795 1106 1 0.12269453291882826 1106 2 0.0522637659591183 1106 3 0.14816607716563368 1106 4 0.0935911252359034 1106 5 -0.06955540395498616 1106 6 0.10464599117427298 1106 7 0.13922481699795242 1106 8 -0.10795565117865635 1106 9 0.2517150487692703 1106 10-11 0.017163016490648886 1106 12-13 0.04494996942617746 1106 14-15 0.05615164802234318 1106 16-17 -0.025872772595462834 1106 18-19 0.08318581101104883 1106 20-21 0.17035161541112842 1106 22-23 -0.018558308640990617 1106 24-25 0.08966532327637822 1106 26-27 0.04938579581507696 1106 28-29 0.10682067339858747 1106 30-31 -0.13121721486614746 1106 32-33 -0.06582457299331423 1106 34-35 0.021923768458471216 1106 36-37 -0.23805355446964427 1106 38-39 -0.26183942938280325 1106 40-41 -0.21554970761728498 1106 42-43 0.061172963406121994 1106 44-45 0.027619145568415604 1106 46-47 -0.42003629392373654 1106 48-49 0.006340192892459129 1106 50-51 -0.12348850261520283 1106 52-53 0.12147574792660265 1106 54-55 0.21490631728111964 1106 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2216 20-21 -0.19873941482728696 2216 22-23 -0.2485901704359108 2216 24-25 -0.28355683702600487 2216 26-27 -0.2853175139348778 2216 28-29 -0.27313728841572527 2216 30-31 -0.5665244449495006 2216 32-33 -0.6274159666742634 2216 34-35 -0.5175400326657709 2216 36-37 -0.6183840448747588 2216 38-39 -0.576277789530117 2216 40-41 -1.0930968339983735 2216 42-43 -1.2628602794704022 2216 44-45 -1.416583340378473 2216 46-47 -0.7402927632007277 2216 48-49 -0.6626605824516005 2216 50-51 -0.36851842604997387 2216 52-53 -0.5868252794042768 2216 54-55 -0.3001068877973836 2216 56-57 -0.49113216093268974 2216 58-59 -0.09680293230412218 2216 60-61 0.07667633128127349 2216 62-63 -0.0625367214967838 2216 64-65 -0.2694353415434101 2216 66-67 0.17370600573347872 2216 68-69 0.0795120868251793 2216 70-71 0.29424388711274574 2216 72-73 0.20747192500040157 2216 74-75 0.16131828145151061 2216 76-77 0.14191699555301795 2216 78-79 0.23808768420527926 2216 80-81 0.14476180337874922 2216 82-83 0.22724323804319724 2216 84-85 0.302781494567256 2216 86-87 0.33967998335276306 2216 88-89 0.24351981446045912 2216 90-91 0.1836035839892709 2216 92-93 0.2259656424092924 2216 94-95 0.3300223182334925 2216 96-97 0.3078252710104685 2216 98-99 0.2303471090561331 2216 100-101 0.04691844455482652 2216 102-103 -0.25554812577442476 2216 104-105 -0.12498427205663276 2216 106-107 -0.2565371844552047 2216 108-109 -0.5213663656045107 2216 110-111 -0.6304330508269018 2216 112-113 -0.5664339212448155 2216 114-115 -0.6519205762066917 2216 116-117 -0.5348585375685282 2216 118-119 -0.39965303107256744 2216 120-121 -0.5316823542542011 2216 122-123 -0.6369423063112976 2216 124-125 -0.9343859323341697 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 17.0 5 58.0 6 147.0 7 343.0 8 618.0 9 1203.0 10 2430.0 11 2914.0 12 3320.0 13 5083.0 14 9499.0 15 9812.0 16 3090.0 17 2058.0 18 1880.0 19 1916.0 20 1674.0 21 1860.0 22 1802.0 23 1462.0 24 1658.0 25 1836.0 26 2036.0 27 2244.0 28 2505.0 29 2801.0 30 3412.0 31 4089.0 32 5031.0 33 6445.0 34 9710.0 35 23180.0 36 24790.0 37 60230.0 38 46450.0 39 268.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.36828895282444 19.812443759356626 15.833733490975268 14.985533796843665 2 15.819774314450896 24.234771066741438 41.99187467472475 17.953579944082914 3 15.739362324453642 35.96093097563632 33.998604101359994 14.301102598550042 4 9.019557177694159 21.26338398927666 39.93637053665154 29.780688296377644 5 7.966336921264544 41.560023883680024 33.18298449174561 17.290654703309826 6 20.740776608923376 43.79612704330948 17.826979957307895 17.636116390459243 7 19.093494704992438 42.576701966717096 22.744528492183562 15.585274836106908 8 25.83439233484619 32.81210287443268 20.278769541099344 21.074735249621785 9 29.561674230963185 13.473726676752396 21.873524962178518 35.091074130105895 10-11 22.116389309127584 30.414321734745336 25.890872415532023 21.578416540595057 12-13 27.725466464952092 30.138981341401916 28.310842158345938 13.82471003530005 14-15 23.481795259707514 26.389309127584472 34.10670700958144 16.022188603126576 16-17 14.73948562783661 31.15501765002521 33.49692385274836 20.608572869389814 18-19 14.753403933434189 22.877054967221383 45.94210791729702 16.4274331820474 20-21 17.340191628845183 21.125163893091276 47.519314170448816 14.015330307614725 22-23 16.55451336359052 22.194452849218358 47.085627836611195 14.16540595057993 24-25 15.135836238058353 22.415198166795765 48.09195875290472 14.357006842241157 26-27 14.94160363086233 22.527281896116992 47.89228441754917 14.638830055471509 28-29 15.078518189795156 21.401728711333448 48.304067615407114 15.21568548346428 30-31 14.953742369407427 21.92325227656939 48.39338957680219 14.729615777220992 32-33 14.889762985375693 22.185980837115483 47.66676752395361 15.257488653555221 34-35 15.380937972768532 21.849117498739286 48.0284417549168 14.741502773575391 36-37 15.717524700148866 21.761143811548656 46.222864128001035 16.298467360301444 38-39 15.594553706505296 22.035501765002522 46.46394351991931 15.90600100857287 40-41 15.465456379223399 22.158144225920324 46.16903681290973 16.207362581946548 42-43 15.786384266263237 22.018759455370652 45.39283913262733 16.80201714573878 44-45 15.893479975550665 22.460910314109405 44.30735961300656 17.33825009733337 46-47 16.91438115756094 22.85892377332914 41.713466147031056 18.513228922078863 48-49 16.980736258194653 23.996167423096317 39.375693393847705 19.64740292486132 50-51 17.70667333691052 24.405427104051768 38.13920368584765 19.74869587319006 52-53 17.369843671205246 25.034997478567828 37.66817952597075 19.926979324256177 54-55 17.01951259856656 25.423263826551096 37.48827489101952 20.06894868386282 56-57 17.555017650025214 25.245990922844175 36.745738779626826 20.453252647503785 58-59 17.246916195144582 24.74285656651235 37.69392921621431 20.31629802212876 60-61 18.211396873424103 25.277660110943017 37.076348966212805 19.43459404942007 62-63 18.868803747254013 24.99258658565368 37.38055325252208 18.758056414570234 64-65 18.51271344569175 24.719456967936498 38.15728161215166 18.610547974220097 66-67 18.520460959074047 25.11875969492626 37.30879941260598 19.051979933393714 68-69 18.947971445112124 24.7862316867273 37.56230496604115 18.703491902119428 70-71 18.71200048413451 25.011599055937705 37.2844088515926 18.991991608335184 72-73 18.67808672357868 24.81582939306405 36.80003066148111 19.70605322187616 74-75 17.648209783156833 25.578618255168934 36.97952597075139 19.793645990922844 76-77 17.066302499480578 25.421236406767218 37.63119196841912 19.881269125333088 78-79 16.7756602144638 24.96227922250821 37.7151294609358 20.54693110209219 80-81 16.485391771625906 25.594455247424097 37.147704075457696 20.772448905492308 82-83 16.750547156300115 24.981189926272577 36.56456444341345 21.70369847401386 84-85 16.61079172970247 24.47644982349975 36.5601613716591 22.35259707513868 86-87 16.16290187883018 24.825813843714574 36.44353625129606 22.56774802615919 88-89 16.452805677318363 24.277878751812995 36.989512372737416 22.27980319813123 90-91 16.35788896106254 24.470767654190485 36.77675613957936 22.394587245167617 92-93 16.564893657796578 24.672238461522944 36.3046210392749 22.458246841405575 94-95 16.29377696818586 24.100556157370146 36.78687492813527 22.818791946308725 96-97 16.5684269132545 24.05623267692233 36.614984890846955 22.76035551897621 98-99 16.304262310955647 24.157735159375207 36.507523445837045 23.030479083832105 100-101 16.381391952328023 24.2055511063683 36.86125865153988 22.5517982897638 102-103 16.85050107315184 24.082777930188648 36.218269401455615 22.8484515952039 104-105 16.541030332248766 24.05641632862189 36.54171617148401 22.860837167645332 106-107 16.189208270297527 24.029248613212303 36.48229954614221 23.299243570347958 108-109 16.35290035465142 24.374921827402975 35.588402616109 23.683775201836603 110-111 16.392060354217936 24.52051478597652 35.42300399402913 23.664420865776414 112-113 15.898862464975258 24.515402817310544 35.465513867679604 24.120220850034595 114-115 15.734140191628846 24.712254160363088 35.35128593040847 24.202319717599597 116-117 15.175623148004332 24.65501695414413 35.53471615676481 24.63464374108672 118-119 15.148232645675867 24.637260182232254 35.53528959678542 24.67921757530646 120-121 14.979354471718551 25.050983461388725 34.94308611817674 25.026575948715983 122-123 15.273157851207994 25.2935516471369 34.18290673116297 25.250383770492135 124-125 14.79842436423662 25.31369438962276 33.866482627243485 26.021398618897134 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2557.0 1 1697.5 2 1404.0 3 1739.0 4 903.0 5 558.0 6 1354.0 7 3174.5 8 5437.0 9 5895.0 10 3531.0 11 1334.0 12 955.5 13 921.5 14 939.0 15 1012.5 16 1095.5 17 1168.0 18 1229.5 19 1206.0 20 1219.5 21 1405.5 22 1579.5 23 1699.5 24 1922.5 25 2322.0 26 2698.5 27 2975.0 28 3225.5 29 3528.5 30 3990.0 31 4354.5 32 5140.5 33 6120.0 34 6820.5 35 7391.5 36 8397.5 37 9390.0 38 9223.5 39 9082.0 40 8977.0 41 8385.0 42 7738.5 43 7674.5 44 7530.5 45 7109.0 46 6835.5 47 6466.5 48 6042.0 49 5477.0 50 5016.5 51 4245.0 52 3529.0 53 3347.5 54 3187.5 55 3172.0 56 3228.0 57 2933.0 58 2712.0 59 2716.0 60 2593.0 61 2508.0 62 2220.0 63 1356.5 64 705.0 65 620.0 66 531.0 67 328.0 68 230.0 69 190.5 70 163.5 71 142.5 72 132.0 73 170.5 74 133.5 75 62.5 76 45.0 77 37.5 78 31.0 79 12.0 80 5.5 81 5.5 82 5.0 83 3.0 84 1.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022592032274331823 2 0.003227433182047403 3 0.0024205748865355524 4 0.07705496722138175 5 0.002824004034291478 6 0.021785173978819967 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0012102874432677762 26-27 0.0 28-29 0.0010085728693898135 30-31 0.00968229954614221 32-33 0.0 34-35 0.0 36-37 8.068582955118507E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0062531517902168435 46-47 0.003630862329803328 48-49 0.0 50-51 0.003227433182047403 52-53 0.0 54-55 0.003832576903681291 56-57 0.0 58-59 0.0030257186081694403 60-61 0.0 62-63 0.005446293494704992 64-65 0.0030257186081694403 66-67 2.0171457387796267E-4 68-69 6.051437216338881E-4 70-71 0.004034291477559254 72-73 0.002824004034291478 74-75 0.0 76-77 0.001412002017145739 78-79 4.0342914775592535E-4 80-81 4.0342914775592535E-4 82-83 0.0010085728693898135 84-85 0.0 86-87 0.003227433182047403 88-89 0.004639435199193141 90-91 0.006454866364094806 92-93 0.007866868381240545 94-95 0.005446293494704992 96-97 0.004841149773071105 98-99 0.006656580937972768 100-101 0.005849722642460918 102-103 0.002824004034291478 104-105 0.001412002017145739 106-107 0.0 108-109 0.01048915784165406 110-111 0.002017145738779627 112-113 0.005849722642460918 114-115 0.0 116-117 2.0171457387796267E-4 118-119 0.001815431164901664 120-121 2.0171457387796267E-4 122-123 0.0022188603126575895 124-125 0.04114977307110439 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 247875.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.418759455370655 #Duplication Level Percentage of deduplicated Percentage of total 1 70.98800305739258 25.852950075642966 2 12.462197999401814 9.07715582450832 3 5.307234721345252 5.798487140695915 4 2.932216720392587 4.271507816439738 5 1.8200347833793051 3.3141704488149273 6 1.2805600788718665 2.798184568835098 7 0.8939550031570901 2.2789712556732225 8 0.7056373444994627 2.0558749369641958 9 0.5040266746424733 1.6520423600605145 >10 2.824764879864411 18.853050932929904 >50 0.17613239839154565 4.311043872919818 >100 0.09083557652897323 6.043772062531518 >500 0.005538754666400806 1.4454866364094807 >1k 0.0066465055996809685 5.54351991931417 >5k 0.0022155018665603226 6.7037821482602125 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9236 3.726071608673727 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7381 2.9777105395864853 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4981 2.0094805849722643 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2294 0.9254664649520927 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2109 0.8508320726172466 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1790 0.7221381744831064 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1514 0.6107917297024711 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1053 0.4248108925869894 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 846 0.34130105900151286 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 739 0.29813414019162887 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 687 0.2771558245083207 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 687 0.2771558245083207 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 624 0.25173978819969745 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 445 0.1795259707513868 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 440 0.17750882501260715 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 421 0.16984367120524457 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 398 0.1605648008068583 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 383 0.15451336359051943 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 357 0.14402420574886535 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 331 0.1335350479072113 RNA PCR Primer, Index 39 (95% over 23bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 317 0.12788703983862834 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 297 0.11981845688350982 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 276 0.1113464447806354 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 261 0.10529500756429652 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 261 0.10529500756429652 No Hit CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT 258 0.10408472012102875 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 254 0.10247100353000504 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.0342914775592535E-4 0.0 10-11 0.0 0.0 0.0 4.0342914775592535E-4 0.0 12-13 0.0 0.0 0.0 6.05143721633888E-4 0.0 14-15 0.0 0.0 0.0 8.068582955118507E-4 0.0 16-17 0.0 0.0 0.0 0.001210287443267776 0.0 18-19 0.0 0.0 0.0 0.0026222894604135145 0.0 20-21 0.0 0.0 0.0 0.004034291477559253 0.0 22-23 0.0 0.0 0.0 0.008673726676752395 0.0 24-25 0.0 0.0 0.0 0.01734745335350479 0.0 26-27 0.0 0.0 0.0 0.026828038325769035 0.0 28-29 0.0 0.0 0.0 0.041553202218860316 0.0 30-31 0.0 0.0 0.0 0.08835098335854766 0.0 32-33 0.0 0.0 0.0 0.15128593040847202 0.0 34-35 0.0 0.0 0.0 0.21765002521432175 0.0 36-37 0.0 0.0 0.0 0.3100353000504287 0.0 38-39 0.0 0.0 0.0 0.4718103883005547 0.0 40-41 0.0 0.0 0.0 0.6910741301059001 0.0 42-43 0.0 0.0 0.0 0.8812909732728189 0.0 44-45 0.0 0.0 0.0 1.0666666666666667 0.0 46-47 0.0 0.0 0.0 1.270600100857287 0.0 48-49 0.0 0.0 0.0 1.5197175995965708 0.0 50-51 0.0 0.0 0.0 1.8319717599596572 0.0 52-53 0.0 0.0 0.0 2.114775592536561 0.0 54-55 0.0 0.0 0.0 2.3584467977811396 0.0 56-57 0.0 0.0 0.0 2.6356026222894604 0.0 58-59 0.0 0.0 0.0 2.968229954614221 0.0 60-61 0.0 0.0 0.0 3.3718608169440243 0.0 62-63 0.0 0.0 0.0 3.7871911245587495 0.0 64-65 0.0 0.0 0.0 4.144024205748865 0.0 66-67 0.0 0.0 0.0 4.491780131114473 0.0 68-69 0.0 0.0 0.0 4.920221886031266 0.0 70-71 0.0 0.0 0.0 5.425920322743318 0.0 72-73 0.0 0.0 0.0 5.979021684316692 0.0 74-75 0.0 0.0 0.0 6.46434694906707 0.0 76-77 0.0 0.0 0.0 6.926071608673727 0.0 78-79 0.0 0.0 0.0 7.489056984367121 0.0 80-81 0.0 0.0 0.0 8.117196167423096 0.0 82-83 0.0 0.0 0.0 8.789712556732225 0.0 84-85 0.0 0.0 0.0 9.451336359051941 0.0 86-87 0.0 0.0 0.0 10.082904689863842 0.0 88-89 0.0 0.0 0.0 10.737065052950076 0.0 90-91 0.0 0.0 0.0 11.495310136157338 0.0 92-93 0.0 0.0 0.0 12.310640443772062 0.0 94-95 0.0 0.0 0.0 13.105799293998992 0.0 96-97 0.0 0.0 0.0 13.872718103883006 0.0 98-99 0.0 0.0 0.0 14.690468986384266 0.0 100-101 0.0 0.0 0.0 15.57720625315179 0.0 102-103 0.0 0.0 0.0 16.536157337367626 0.0 104-105 0.0 0.0 0.0 17.50862329803328 0.0 106-107 0.0 0.0 0.0 18.425819465456378 0.0 108-109 0.0 0.0 0.0 19.31699445284922 0.0 110-111 0.0 0.0 0.0 20.297327281896116 0.0 112-113 0.0 0.0 0.0 21.378920827029752 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2040 0.0 98.967476 1 GTATCAA 4590 0.0 65.524185 1 TAGATAG 65 7.456038E-9 64.05922 5 AGCCGGT 15 0.004232999 59.507572 102-103 GACGCTG 15 0.0042363834 59.495564 68-69 GATAGTC 80 3.8387952E-8 52.04811 7 GTGGTAT 380 0.0 51.71938 1 AGATAGT 85 6.188566E-8 48.986458 6 ATCAACG 6215 0.0 48.477993 3 TATCAAC 6240 0.0 48.188343 2 TCAACGC 6270 0.0 48.052742 4 GTATAGA 100 4.090907E-9 47.64452 1 CAACGCA 6380 0.0 47.176617 5 AACGCAG 6500 0.0 46.39717 6 TGGTATC 425 0.0 46.233868 2 CGACCGT 50 2.2269523E-7 41.638493 18-19 ACGCAGA 7330 0.0 41.06232 7 CGCAGAG 7380 0.0 40.70352 8 GCAGAGT 7700 0.0 38.62569 9 GTTCGAC 55 4.70307E-7 37.853172 16-17 >>END_MODULE