FastQCFastQC Report
Fri 27 May 2016
SRR940153_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940153_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2004981
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG44970.22429140226266484No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA40740.2031939454787851No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37720.18813145860235084No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA34450.17182207711694025No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA34250.1708245614297592No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC33480.16698412603411206No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT31130.15526331670973442No Hit
CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA28960.14444027150381972No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA28570.14249511591381664No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA28130.1403005814020183No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA27240.13586163659406247No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG26360.13147256757046574No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG25070.12503859138814782No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT23810.11875424255890704No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG23140.11541256500685045No Hit
GGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTG22470.11207088745479384No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT22300.11122299912068993No Hit
CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT21110.10528778078196252No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA20740.10344237676067752No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38650.062.0670621
GGTATCA26200.048.8475531
ATCAACG50250.046.79033
TCAACGC51400.045.5118184
CAACGCA52800.044.3039635
AACGCAG55250.042.4312066
TATCAAC56150.042.1918072
GTACATG110150.038.7471961
TAAGGTA10600.037.062344
TACATGG115750.036.5117072
ACATGGG113450.036.3075033
ACGCAGA65100.035.824757
CGCAGAG67300.034.5669828
GTGTAAG10700.032.266351
CATGGGG54250.032.2583664
TAAGGTG15000.030.9518995
CATGGGA66650.030.9008734
AGGTAAG13900.029.1198352
GGTAAGG15700.028.8143423
CCGTTGC3550.028.4918299
Produced by FastQC (version 0.11.5)