FastQCFastQC Report
Fri 27 May 2016
SRR940152_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940152_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2353509
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG26530.11272529656780578No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26460.11242786834467172No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25150.063.201111
GTATCAA46600.057.6158681
TCAACGC56350.046.2734264
ATCAACG56850.045.9711653
CAACGCA58650.044.4587825
AACGCAG60600.042.8162236
TATCAAC64650.041.5297662
GTACATG125550.039.0716671
TACATGG129150.036.9223672
ACATGGG128450.035.686843
ACGCAGA76500.033.830037
CGCAGAG77500.033.0906648
CATGGGG66700.031.5065124
GCAGAGT88050.027.919
CATGGGA68300.026.9332794
TAAGGTG13700.024.7688435
ATGGGGA39350.023.2984715
GTAAGGT13050.023.2654324
GAGTACA93550.023.163761
CAGAGTA84350.022.2558510-11
Produced by FastQC (version 0.11.5)