##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940152_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2353509 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.716242852693572 33.0 31.0 34.0 30.0 34.0 2 31.796755822901037 34.0 31.0 34.0 30.0 34.0 3 31.871009203703917 34.0 31.0 34.0 30.0 34.0 4 35.18885544945866 37.0 35.0 37.0 33.0 37.0 5 35.2173104075659 37.0 35.0 37.0 33.0 37.0 6 35.30481591529924 37.0 36.0 37.0 33.0 37.0 7 35.26252714563658 37.0 36.0 37.0 33.0 37.0 8 35.23952914562893 37.0 36.0 37.0 33.0 37.0 9 36.883215233083874 39.0 38.0 39.0 33.0 39.0 10-11 36.83192118661964 39.0 38.0 39.0 33.0 39.0 12-13 36.76018489837939 39.0 38.0 39.0 33.0 39.0 14-15 38.21411177947482 41.0 39.0 41.0 33.0 41.0 16-17 38.22680814052549 41.0 39.0 41.0 33.0 41.0 18-19 38.24469037509523 41.0 39.0 41.0 33.0 41.0 20-21 38.284829588499555 41.0 39.0 41.0 33.0 41.0 22-23 38.29181617746097 41.0 39.0 41.0 33.0 41.0 24-25 38.30561960884789 41.0 39.0 41.0 33.0 41.0 26-27 38.282589316633164 41.0 39.0 41.0 33.0 41.0 28-29 38.21332253243986 41.0 38.0 41.0 33.0 41.0 30-31 38.13209297266337 41.0 38.0 41.0 33.0 41.0 32-33 38.05078034543314 40.0 38.0 41.0 33.0 41.0 34-35 37.96815563484142 40.0 38.0 41.0 33.0 41.0 36-37 37.839748435208875 40.0 38.0 41.0 32.5 41.0 38-39 37.699216786509 40.0 38.0 41.0 32.0 41.0 40-41 37.577848650674376 40.0 38.0 41.0 32.0 41.0 42-43 37.54763907849939 40.0 38.0 41.0 31.5 41.0 44-45 37.62673841485204 40.0 38.0 41.0 32.0 41.0 46-47 37.532661018079814 40.0 38.0 41.0 32.0 41.0 48-49 37.415730723783085 40.0 37.0 41.0 32.0 41.0 50-51 37.278624385970055 40.0 37.0 41.0 31.0 41.0 52-53 37.102320832425114 40.0 36.5 41.0 31.0 41.0 54-55 36.912268021919616 40.0 36.0 41.0 31.0 41.0 56-57 36.709206763177875 40.0 35.5 41.0 30.0 41.0 58-59 36.46377685405069 39.0 35.0 41.0 30.0 41.0 60-61 36.21342833190781 39.0 35.0 41.0 30.0 41.0 62-63 35.92565824052511 39.0 35.0 41.0 30.0 41.0 64-65 35.61487570262106 38.0 35.0 40.5 30.0 41.0 66-67 35.27990693895795 37.0 35.0 40.0 29.0 41.0 68-69 34.94413469419493 37.0 34.5 40.0 29.0 41.0 70-71 34.83005949839155 36.0 35.0 39.0 29.5 41.0 72-73 34.60088489145357 36.0 35.0 39.0 30.0 41.0 74-75 34.26746997780761 35.5 35.0 38.5 29.0 40.5 76-77 33.9149523116334 35.0 34.0 37.0 29.0 39.5 78-79 33.59056306986716 35.0 34.0 37.0 29.0 39.0 80-81 33.28851026276084 35.0 34.0 36.5 29.0 39.0 82-83 33.01611699806544 35.0 34.0 36.0 29.0 37.0 84-85 32.7761788886297 35.0 34.0 36.0 28.0 37.0 86-87 32.57617837875274 35.0 34.0 35.0 28.0 37.0 88-89 32.40789030337254 35.0 34.0 35.0 27.5 36.0 90-91 32.278986823504816 35.0 34.0 35.0 27.0 36.0 92-93 32.14529602393702 35.0 34.0 35.0 27.0 36.0 94-95 32.05923686716304 35.0 34.0 35.0 27.0 36.0 96-97 31.943748674851044 35.0 34.0 35.0 27.0 35.0 98-99 31.865079334729543 35.0 34.0 35.0 26.0 35.0 100-101 31.80438336968331 35.0 34.0 35.0 25.5 35.0 102-103 31.016313938038905 34.5 32.0 35.0 24.5 35.0 104-105 31.616715721078613 35.0 33.0 35.0 25.0 35.0 106-107 31.684429505049692 35.0 34.0 35.0 25.0 35.0 108-109 31.650167260885766 35.0 34.0 35.0 25.0 35.0 110-111 31.571265076955306 35.0 33.5 35.0 25.0 35.0 112-113 31.478798466460084 35.0 33.0 35.0 25.0 35.0 114-115 31.358117602269633 35.0 33.0 35.0 24.0 35.0 116-117 31.285572309262466 35.0 33.0 35.0 24.0 35.0 118-119 31.158877871297708 35.0 33.0 35.0 23.0 35.0 120-121 31.01827505227301 35.0 33.0 35.0 21.5 35.0 122-123 30.80441035067212 35.0 33.0 35.0 12.0 35.0 124-125 28.78536644644231 33.5 29.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1210411001918672 1101 2 0.16698193111623283 1101 3 0.20488677233482022 1101 4 0.19752482667681193 1101 5 0.13679001031572824 1101 6 0.21226494630201387 1101 7 0.2810472941015618 1101 8 0.27122637930143156 1101 9 0.3380744012713066 1101 10-11 0.3126889867196283 1101 12-13 0.2791235862738546 1101 14-15 0.3128485727659509 1101 16-17 0.32784588926561753 1101 18-19 0.35063030193773415 1101 20-21 0.37831426493944065 1101 22-23 0.2804387645197224 1101 24-25 0.347775226352546 1101 26-27 0.41221777575086804 1101 28-29 0.41245637358238696 1101 30-31 0.35258660200128134 1101 32-33 0.37907541538022116 1101 34-35 0.4097599428600063 1101 36-37 0.34823543921601896 1101 38-39 0.3733323052227604 1101 40-41 0.4390828322108433 1101 42-43 0.4245310406892102 1101 44-45 0.27006501179342735 1101 46-47 0.2630007125959537 1101 48-49 0.24033591778977126 1101 50-51 0.24432889881277475 1101 52-53 0.21068964048193095 1101 54-55 0.25235507290497594 1101 56-57 0.2187481844160999 1101 58-59 0.31134903347860643 1101 60-61 0.24714276615795683 1101 62-63 0.2904104033980275 1101 64-65 0.20218604869865686 1101 66-67 0.18203173586538668 1101 68-69 0.18912560092759634 1101 70-71 0.24714169286092158 1101 72-73 0.28665713140441085 1101 74-75 0.2786590133390803 1101 76-77 0.2642734668882696 1101 78-79 0.3016058676964448 1101 80-81 0.25502595303692743 1101 82-83 0.1817416672075467 1101 84-85 0.1868248920751583 1101 86-87 0.1363158044785422 1101 88-89 0.07088452630654274 1101 90-91 0.03912048228153964 1101 92-93 -0.005795701945338294 1101 94-95 -0.007792523871749779 1101 96-97 0.003227296200499552 1101 98-99 0.03661607824803781 1101 100-101 -0.0130616411120279 1101 102-103 -0.020732915255035067 1101 104-105 -0.09381907882964313 1101 106-107 -0.03908669564237144 1101 108-109 -0.021616297185534705 1101 110-111 -0.08394947732842084 1101 112-113 -0.04086185884189675 1101 114-115 -0.016635403513543423 1101 116-117 -2.6405962898934376E-4 1101 118-119 -0.009140930400857172 1101 120-121 -0.059760307010396474 1101 122-123 -0.16255995558229586 1101 124-125 -0.12669396105879116 1102 1 0.07091870968529435 1102 2 0.08293905293904302 1102 3 0.0493522041117771 1102 4 0.05352950527679923 1102 5 -0.03240590223221318 1102 6 0.012959338295843281 1102 7 0.12383669523948981 1102 8 0.09906579851071484 1102 9 0.08109743175080553 1102 10-11 0.06408971046676726 1102 12-13 0.011031844095768406 1102 14-15 0.18500991886747187 1102 16-17 0.10109646395480354 1102 18-19 0.10716038461954014 1102 20-21 0.1309915544236233 1102 22-23 0.19629211384319234 1102 24-25 0.12922842946520774 1102 26-27 0.046646165574308895 1102 28-29 0.21722467892827524 1102 30-31 0.2310967022360373 1102 32-33 0.045515753527318736 1102 34-35 0.06461046900661671 1102 36-37 0.12159169968152383 1102 38-39 0.07462931569102693 1102 40-41 0.09992250752591758 1102 42-43 0.04848355570547369 1102 44-45 -0.06353159452697099 1102 46-47 -0.02788886371615007 1102 48-49 -0.051556124554146265 1102 50-51 -0.051927419442812095 1102 52-53 -0.009625388773436327 1102 54-55 0.02838386243931268 1102 56-57 0.0094767721361535 1102 58-59 -0.1598036238854661 1102 60-61 -0.042240696733081506 1102 62-63 -0.09948547151302023 1102 64-65 -0.1211966650988785 1102 66-67 -0.1257662891407776 1102 68-69 -0.1903527085701029 1102 70-71 -0.1429536639529303 1102 72-73 -0.21562016930433003 1102 74-75 -0.1389387434985352 1102 76-77 -0.1123864945017985 1102 78-79 -0.08844976584286712 1102 80-81 -0.172182102988927 1102 82-83 -0.19904208939884427 1102 84-85 -0.1640272404952583 1102 86-87 -0.18711542455683627 1102 88-89 -0.06609640278622919 1102 90-91 -0.14066154627128924 1102 92-93 -0.11265399818311295 1102 94-95 -0.0727347479177638 1102 96-97 -0.08421925617914638 1102 98-99 -0.014386601888585204 1102 100-101 -0.024949878767994704 1102 102-103 -0.08774790158922485 1102 104-105 -0.14147047210982677 1102 106-107 -0.1906759045050741 1102 108-109 -0.19938815494013085 1102 110-111 -0.26438967316416395 1102 112-113 -0.2290491409617843 1102 114-115 -0.23975939670847524 1102 116-117 -0.29354158783547746 1102 118-119 -0.2195478270172977 1102 120-121 -0.21284633623451654 1102 122-123 -0.21068310830197845 1102 124-125 -0.3708383085142657 1103 1 0.010822048960971387 1103 2 0.08649470533055847 1103 3 0.14503902810548297 1103 4 0.03680472943660362 1103 5 -0.0012553132472277184 1103 6 0.11365149763698668 1103 7 -0.05779375594265446 1103 8 0.00831165250588839 1103 9 0.15819071805589857 1103 10-11 0.11052564823669542 1103 12-13 0.10200792779121315 1103 14-15 0.2525256525586528 1103 16-17 -0.04622968599375099 1103 18-19 0.12196548376218885 1103 20-21 0.10188280130867611 1103 22-23 0.06144142380340156 1103 24-25 0.0773948339606676 1103 26-27 0.04851793582025721 1103 28-29 -0.034645290837332254 1103 30-31 0.04926965683338835 1103 32-33 -0.006119515649039897 1103 34-35 0.13906156180701856 1103 36-37 0.07197374337713569 1103 38-39 -0.07758069415919522 1103 40-41 0.04168863743673512 1103 42-43 0.008197283889472828 1103 44-45 0.035683122553756164 1103 46-47 0.01055303250416273 1103 48-49 0.0934014593197432 1103 50-51 0.07205329217082834 1103 52-53 0.022097889705264606 1103 54-55 0.10028046108124755 1103 56-57 0.026139602336272105 1103 58-59 0.08642496236370079 1103 60-61 -0.02502245253416646 1103 62-63 -0.058602990004409605 1103 64-65 0.025701289601030908 1103 66-67 0.02817806188634364 1103 68-69 0.027955076934802037 1103 70-71 -0.00877225120033387 1103 72-73 -0.15242212421574663 1103 74-75 -0.050942249649111204 1103 76-77 -0.18173589207020058 1103 78-79 -0.06530816923159932 1103 80-81 -0.1559723556897552 1103 82-83 -0.12371363848198769 1103 84-85 -0.0953244660690018 1103 86-87 -0.2679218027242598 1103 88-89 -0.13485729398296797 1103 90-91 -0.01740011515835249 1103 92-93 -0.005837926594189469 1103 94-95 -0.07082614759293904 1103 96-97 -0.2982989212806899 1103 98-99 -0.16463062395199302 1103 100-101 -0.24190899054318393 1103 102-103 -0.15898693746163417 1103 104-105 -0.08017031562757637 1103 106-107 -0.026120251657740567 1103 108-109 -0.02378347843175277 1103 110-111 -0.013128448080873767 1103 112-113 -0.020912610765449813 1103 114-115 -0.02744990006351955 1103 116-117 -0.03985618690497006 1103 118-119 0.003231257691428624 1103 120-121 -0.054158061372731936 1103 122-123 -0.13461135528594426 1103 124-125 0.15814290677805687 1104 1 0.03998706820718212 1104 2 0.09766392432086946 1104 3 0.09408802706474617 1104 4 0.05446860719433744 1104 5 0.020407162501470566 1104 6 0.1278265988720051 1104 7 0.08283591844927685 1104 8 0.05611558306784303 1104 9 0.08755477411651214 1104 10-11 0.23535130589965547 1104 12-13 0.1635056809415829 1104 14-15 0.0997818391928007 1104 16-17 0.2030262035437289 1104 18-19 0.14597031090982426 1104 20-21 0.19243221023441492 1104 22-23 0.22427108553955577 1104 24-25 0.11399602118049046 1104 26-27 0.09581168588476885 1104 28-29 0.2360909120069863 1104 30-31 0.2890446622076155 1104 32-33 0.2517291993181132 1104 34-35 0.20968897880283066 1104 36-37 0.34881003189479287 1104 38-39 0.2131598978171283 1104 40-41 0.29725845941658946 1104 42-43 0.30452454977886134 1104 44-45 0.3319839100394333 1104 46-47 0.14252211470434872 1104 48-49 0.24768932265168786 1104 50-51 0.26935653406989957 1104 52-53 0.20622911308380765 1104 54-55 0.23512307838618796 1104 56-57 0.2539850995042059 1104 58-59 0.1995038128060429 1104 60-61 0.2001814872727934 1104 62-63 0.13382674461466593 1104 64-65 0.06543971887519717 1104 66-67 0.1799743523068642 1104 68-69 0.19680718427914456 1104 70-71 0.19180461897156675 1104 72-73 0.24097990636366973 1104 74-75 0.17516734132237843 1104 76-77 -0.03980549975450032 1104 78-79 0.1670251929736679 1104 80-81 0.14131522356032633 1104 82-83 0.11809679320126065 1104 84-85 0.12055853708461939 1104 86-87 0.19406405062757415 1104 88-89 0.1599545096406274 1104 90-91 0.1094611483282435 1104 92-93 0.1407381689722058 1104 94-95 0.18753113224025242 1104 96-97 0.246007705814975 1104 98-99 0.23814417812194222 1104 100-101 0.15584837841901233 1104 102-103 0.1533238597582276 1104 104-105 0.21440008573565805 1104 106-107 0.19427696030098573 1104 108-109 0.13611170299243724 1104 110-111 0.2089807946322928 1104 112-113 0.25286923680780404 1104 114-115 0.25931866208286536 1104 116-117 0.2977968661222974 1104 118-119 0.2640769240518708 1104 120-121 0.260324683010591 1104 122-123 0.27370703719670075 1104 124-125 0.4211116923303635 1105 1 -0.017654025657808603 1105 2 0.03041370002451771 1105 3 0.06904773143778087 1105 4 0.00838459322972085 1105 5 0.023730961123924033 1105 6 0.19287037761666426 1105 7 0.13892506436037877 1105 8 0.1861601314720076 1105 9 0.2527528468502638 1105 10-11 0.3098297059334243 1105 12-13 0.21825682039531102 1105 14-15 0.2713328042080505 1105 16-17 0.23297068296555778 1105 18-19 0.25928401608599216 1105 20-21 0.2040825500812531 1105 22-23 0.2311507287213459 1105 24-25 0.2518499861721537 1105 26-27 0.2539049580186159 1105 28-29 0.22622416863636374 1105 30-31 0.10996233831458113 1105 32-33 0.16154056076408807 1105 34-35 0.2238748234443051 1105 36-37 0.2022597093664018 1105 38-39 0.3021664930439698 1105 40-41 0.34250145589045644 1105 42-43 0.2548554592072918 1105 44-45 0.2679619282461445 1105 46-47 0.17244672339894862 1105 48-49 0.16608108490169826 1105 50-51 0.2605965018916905 1105 52-53 0.24140983823111384 1105 54-55 0.08765468410256716 1105 56-57 0.11971735920595705 1105 58-59 0.11299399653432829 1105 60-61 0.20104489244955204 1105 62-63 0.22765985117148801 1105 64-65 0.13619478619766312 1105 66-67 -0.051476390059093546 1105 68-69 -0.0038237510518825957 1105 70-71 0.08717896978387074 1105 72-73 -0.006858666321107876 1105 74-75 -0.06306872450677758 1105 76-77 0.038189577427381494 1105 78-79 0.0858938085200478 1105 80-81 0.09890349661661446 1105 82-83 0.07554173207846304 1105 84-85 0.07997593266107117 1105 86-87 0.1546657857041538 1105 88-89 0.012949014987128749 1105 90-91 0.1758620535855826 1105 92-93 0.22064918167365732 1105 94-95 0.24391312851356162 1105 96-97 0.2613838511747346 1105 98-99 0.30101891243927525 1105 100-101 0.19499216556236476 1105 102-103 0.24025789397773778 1105 104-105 0.23594030731104354 1105 106-107 0.32792535211003937 1105 108-109 0.27243725580269285 1105 110-111 0.30555601738976534 1105 112-113 0.2791027475263803 1105 114-115 0.25704417032970284 1105 116-117 0.30627106892859146 1105 118-119 0.33094774992963494 1105 120-121 0.3451515811573955 1105 122-123 0.33740335984926517 1105 124-125 0.38014377242723185 1106 1 -0.11789235751103533 1106 2 -0.12409779207523997 1106 3 -0.12876980020205409 1106 4 0.1006996971958074 1106 5 0.12469188605702186 1106 6 0.11370788079065619 1106 7 0.06689333306870537 1106 8 0.15181768288807262 1106 9 0.15240940579118956 1106 10-11 0.15884813859903346 1106 12-13 0.1822775030957473 1106 14-15 0.20735906737863985 1106 16-17 0.1921729154327565 1106 18-19 0.23638268275173147 1106 20-21 0.1586006191387881 1106 22-23 0.2929407236682522 1106 24-25 0.20543166740829832 1106 26-27 0.2646884647181764 1106 28-29 0.1366178292166751 1106 30-31 0.25795217490858136 1106 32-33 0.22398314867660218 1106 34-35 0.2657171409688317 1106 36-37 0.2273388531093019 1106 38-39 0.21604415308102176 1106 40-41 0.23561202283730864 1106 42-43 0.2172654414591051 1106 44-45 0.06731189977070784 1106 46-47 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84-85 -0.2566037589496659 2216 86-87 -0.3814010581199625 2216 88-89 -0.2361030989307764 2216 90-91 -0.2767113447992102 2216 92-93 -0.33523233760938 2216 94-95 -0.20971905765702914 2216 96-97 -0.1618988637071226 2216 98-99 -0.1975274958228539 2216 100-101 -0.3429307832417656 2216 102-103 -0.31696966056005493 2216 104-105 -0.27034870738530614 2216 106-107 -0.3857680519368394 2216 108-109 -0.3433267005424838 2216 110-111 -0.3187575756101175 2216 112-113 -0.29275748107478705 2216 114-115 -0.2852532062634161 2216 116-117 -0.3767492094929281 2216 118-119 -0.452185403366304 2216 120-121 -0.4916099410218919 2216 122-123 -0.6192395588078057 2216 124-125 -0.8772270050978008 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26000.0 3 4967.0 4 3479.0 5 2861.0 6 3135.0 7 3740.0 8 3958.0 9 4524.0 10 5046.0 11 6098.0 12 6901.0 13 7097.0 14 6987.0 15 6930.0 16 6241.0 17 6282.0 18 5468.0 19 5493.0 20 5601.0 21 5665.0 22 6561.0 23 7881.0 24 10931.0 25 15274.0 26 20219.0 27 21019.0 28 23200.0 29 30686.0 30 34946.0 31 40295.0 32 51957.0 33 69816.0 34 102066.0 35 164928.0 36 369974.0 37 824413.0 38 430464.0 39 2406.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.54073107005224 18.39238875261151 13.055386979588993 24.011493197747257 2 15.292807818973762 20.04019972378625 39.79483692765324 24.872155529586742 3 18.55473887419788 25.27299003820607 29.298841494740767 26.87342959285528 4 12.35345622707431 16.388335153928875 37.29456669117741 33.96364192781941 5 13.468786818021853 38.8146318325323 33.96999644676868 13.746584902677164 6 32.33758362963007 36.289458182847454 17.817711844021126 13.555246343501349 7 29.204980098050086 32.61132195095626 20.933910738469212 17.24978721252444 8 27.384166951703744 34.51288402992146 19.854769183703386 18.24817983467141 9 27.464385333607567 15.890319915826833 19.32294313721824 37.32235161334736 10-11 25.121117278746922 26.5361563014127 27.499783301834203 20.842943118006175 12-13 25.97308178505795 23.75503787033791 27.6084217682739 22.663458576330243 14-15 22.82380438882131 25.145343185427965 25.039803241417047 26.991049184333683 16-17 23.09074878636909 28.56418700007436 25.71057691286289 22.634487300693657 18-19 23.22068778839686 27.39359566160189 26.88216729535535 22.5035492546459 20-21 23.902151018741478 26.4976462145036 26.807702587230708 22.79250017952421 22-23 23.694871007175262 26.5962057409742 26.704384910492994 23.00453834135754 24-25 23.33187441705317 26.848932052036567 26.710129624003283 23.109063906906975 26-27 23.025381293007662 27.17661530355788 26.39087233721619 23.407131066218255 28-29 23.061454294364477 27.31802198969565 26.488659903330326 23.13186381260955 30-31 23.067665128381844 26.802548728758644 26.634882623103508 23.494903519756004 32-33 22.853292616159838 27.030485404427207 26.612570669570744 23.503651309842212 34-35 23.518003250895315 27.01408600914678 26.308988867882398 23.158921872075506 36-37 23.620670423702254 26.634103330872318 26.16010070696716 23.58512553845827 38-39 23.201716601426607 26.83020411198275 26.264299258016027 23.703780028574613 40-41 23.478005159758304 26.676953320377983 26.46483889459976 23.380202625263948 42-43 23.364947840312748 26.674400322943885 26.34605987209722 23.61459196464614 44-45 23.16250781285519 26.760394018592788 26.55465726900399 23.522440899548034 46-47 23.52446992592391 27.0938010994129 26.016995176045445 23.364733798617745 48-49 23.328151546961877 27.034371666442524 26.020619643070734 23.61685714352486 50-51 23.338383680642977 26.965157702778242 26.295035893160403 23.401422723418378 52-53 23.70709829428905 26.662863686532457 26.371670135027042 23.25836788415145 54-55 23.152632122384098 26.785267372101213 26.433909807885726 23.628190697628963 56-57 23.18892680748247 27.08639022779627 26.3551364538197 23.36954651090156 58-59 23.46305503608748 26.569550867227786 26.27451472025325 23.69287937643149 60-61 23.028990458320163 26.74593705278687 26.737672639139582 23.487399849753384 62-63 23.144752488836698 26.861143340464277 26.739402432736025 23.254701737963003 64-65 22.929017213716584 26.97204385690588 26.49506771272401 23.603871216653523 66-67 22.872097010425843 27.141053733642835 26.311282148682864 23.67556710724846 68-69 23.148162114699915 26.709911359921882 26.764871904963933 23.377054620414263 70-71 23.01211333826946 26.732861745625208 26.60668246651915 23.64834244958618 72-73 23.068199562565926 26.84211476748966 26.39928446917832 23.690401200766097 74-75 22.92305335616306 27.265325877058146 26.45727000640997 23.354350760368824 76-77 23.18343166097722 26.843298046436598 26.484083075420696 23.489187217165487 78-79 23.186178750381085 26.89297008363156 26.51395647504597 23.40689469094138 80-81 23.25671862365467 27.16415804906984 26.41514204215905 23.163981285116435 82-83 23.164369816312167 27.058417621760828 26.480761545738822 23.29645101618818 84-85 23.215361236213933 27.068629665029892 26.410675805929746 23.305333292826433 86-87 23.299399758781767 26.84310255854068 26.531753850324446 23.325743832353112 88-89 23.12600731448948 27.067392818339 26.348457621724382 23.458142245447142 90-91 23.127599714963523 27.129328290380272 26.523328332022455 23.21974366263375 92-93 23.150142678358574 26.671079107341296 26.69931731114506 23.479460903155072 94-95 22.884127487933974 26.660977289655573 26.82069624547856 23.63419897693189 96-97 23.170899335223993 26.5770186056976 26.727581628974217 23.524500430104194 98-99 22.939767902884885 26.71069272849762 26.554454313860752 23.795085054756743 100-101 22.6788807690984 26.67319309167715 26.98646999012963 23.66145614909482 102-103 22.844928865731013 26.350888270143237 26.96671392946781 23.837468934657938 104-105 22.718118374139756 26.581054520059162 26.798108974679035 23.902718131122054 106-107 22.87276148083564 26.644703716875522 26.63949872297068 23.843036079318157 108-109 22.906498339288273 26.50822665220316 26.459703362086145 24.12557164642243 110-111 22.73128415921642 26.74396569196274 26.82289066373883 23.70185948508201 112-113 22.70760663063057 27.01077726046059 26.885276969602458 23.396339139306384 114-115 22.68633390929469 27.006426505015067 26.55472918237495 23.752510403315295 116-117 22.637305346133818 26.779256211322306 26.85529175746212 23.728146685081757 118-119 22.546303456973522 27.097104259901045 26.46792624080687 23.88866604231856 120-121 22.574338746695567 27.193907370255747 26.687292978251442 23.544460904797244 122-123 22.532624179017912 27.16509295169019 26.716124762721172 23.586158106570725 124-125 22.608650171864266 27.128764361089512 26.505983623409428 23.75660184363679 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 297.0 1 295.5 2 331.5 3 353.0 4 286.5 5 237.0 6 247.0 7 239.0 8 226.0 9 245.0 10 243.0 11 199.0 12 203.0 13 250.0 14 274.0 15 302.5 16 362.0 17 451.0 18 537.0 19 673.5 20 971.5 21 1422.5 22 2109.0 23 3094.5 24 4282.5 25 6152.5 26 8598.0 27 11673.5 28 14671.0 29 18358.5 30 23536.0 31 28696.5 32 36084.0 33 40446.5 34 42521.0 35 45801.5 36 50461.5 37 57190.5 38 61277.0 39 63508.5 40 65964.0 41 70198.0 42 73778.0 43 77703.0 44 82686.0 45 87896.5 46 89977.5 47 89352.0 48 89651.5 49 88016.5 50 85876.5 51 83336.5 52 80946.0 53 82209.0 54 85251.5 55 82315.5 56 74918.0 57 69408.0 58 67339.0 59 62414.5 60 51458.5 61 41648.5 62 34780.0 63 26948.5 64 20512.5 65 16537.0 66 12120.5 67 9569.0 68 7185.5 69 4504.5 70 3117.5 71 2243.5 72 1711.5 73 1303.0 74 1017.5 75 866.0 76 611.5 77 340.5 78 193.5 79 118.0 80 96.5 81 77.5 82 63.5 83 54.0 84 47.5 85 35.5 86 24.0 87 20.0 88 14.5 89 13.0 90 11.5 91 11.5 92 11.0 93 7.0 94 5.0 95 5.0 96 3.0 97 5.0 98 5.5 99 4.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019757731965333467 2 0.01100484425595993 3 0.009262764663317625 4 0.03394930718344395 5 0.030635106982807372 6 0.024431604043154285 7 0.009432723647965654 8 5.948564462681043E-4 9 0.011259782732931975 10-11 2.1244873081003728E-4 12-13 0.0035691386776086262 14-15 0.01578494069918577 16-17 0.0014446513695082536 18-19 0.008412969740077476 20-21 0.002804323246692492 22-23 2.549384769720447E-4 24-25 0.006968318370569222 26-27 1.699589846480298E-4 28-29 0.005693625985708999 30-31 9.347744155641639E-4 32-33 0.0016358552272372868 34-35 1.4871411156702608E-4 36-37 0.006224747812734092 38-39 9.135295424831602E-4 40-41 0.012661944356278222 42-43 0.006755869639759185 44-45 6.798359385921192E-4 46-47 0.010749905778987887 48-49 0.003122996342907548 50-51 0.008731642836292531 52-53 0.003887811773823682 54-55 0.002804323246692492 56-57 0.009432723647965654 58-59 0.017314571561018035 60-61 0.0019120385772903353 62-63 0.006394706797382122 64-65 0.0015721206079942758 66-67 0.0012746923848602236 68-69 1.0622436540501864E-4 70-71 0.004737606697063831 72-73 4.886320808630857E-4 74-75 0.006139768320410076 76-77 0.0136816982641664 78-79 0.0013384270041032348 80-81 0.0037390976622566556 82-83 0.0013809167502652421 84-85 2.1244873081003726E-5 86-87 0.0016358552272372868 88-89 0.00416399512387673 90-91 0.005353708016412939 92-93 0.013341780294870341 94-95 0.0 96-97 4.0365258853907083E-4 98-99 0.002315691165829406 100-101 0.0 102-103 8.49794923240149E-5 104-105 0.0077543786745663595 106-107 0.0 108-109 0.0 110-111 3.399179692960596E-4 112-113 0.005438687508736954 114-115 0.0022307116735053915 116-117 1.9120385772903356E-4 118-119 0.005141259285602902 120-121 0.0018058142118853168 122-123 6.373461924301118E-5 124-125 0.07641780847237041 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2353509.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.39461511303258 #Duplication Level Percentage of deduplicated Percentage of total 1 65.78625396877128 25.258379008591852 2 14.832291236524838 11.389602265415547 3 6.482941879321978 7.467301747701824 4 3.5773739638371724 5.49407585827648 5 2.233112359280574 4.286974476936689 6 1.4707153612596167 3.388053014183259 7 1.0216627916989618 2.745844476381161 8 0.7586962038689877 2.330387898821495 9 0.564533780967096 1.9507551514683024 >10 2.9615741138294807 20.364193419097695 >50 0.20186923389330663 5.324668925861281 >100 0.10111646817610258 6.8596956057926635 >500 0.00498082726308837 1.3372137186078457 >1k 0.0028778113075621364 1.8028544328640488 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2653 0.11272529656780578 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2646 0.11242786834467172 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.248974616200745E-5 0.0 6 0.0 0.0 0.0 4.248974616200745E-5 0.0 7 0.0 0.0 0.0 4.248974616200745E-5 0.0 8 0.0 0.0 0.0 4.248974616200745E-5 0.0 9 0.0 0.0 0.0 4.248974616200745E-5 0.0 10-11 0.0 0.0 0.0 6.373461924301118E-5 0.0 12-13 0.0 0.0 0.0 8.49794923240149E-5 0.0 14-15 0.0 0.0 0.0 8.49794923240149E-5 0.0 16-17 0.0 0.0 0.0 8.49794923240149E-5 0.0 18-19 0.0 0.0 0.0 8.49794923240149E-5 0.0 20-21 0.0 0.0 0.0 1.2746923848602236E-4 0.0 22-23 0.0 0.0 0.0 2.9742822313405217E-4 0.0 24-25 0.0 0.0 0.0 4.2489746162007456E-4 0.0 26-27 0.0 0.0 0.0 5.948564462681043E-4 0.0 28-29 0.0 0.0 0.0 0.0014658962425892572 0.0 30-31 0.0 0.0 0.0 0.0048013413163068425 0.0 32-33 0.0 0.0 0.0 0.011366007098336994 0.0 34-35 0.0 0.0 0.0 0.019693997346090453 0.0 36-37 0.0 0.0 0.0 0.033375695610256856 0.0 38-39 0.0 0.0 0.0 0.0592731958960004 0.0 40-41 0.0 0.0 0.0 0.09247893252160923 0.0 42-43 0.0 0.0 0.0 0.12570591402029907 2.1244873081003726E-5 44-45 0.0 0.0 0.0 0.16322435988135164 4.248974616200745E-5 46-47 0.0 0.0 0.0 0.20728622665135338 4.248974616200745E-5 48-49 0.0 0.0 0.0 0.2550447013374497 4.248974616200745E-5 50-51 0.0 0.0 0.0 0.31448785621809816 4.248974616200745E-5 52-53 0.0 0.0 0.0 0.3692783838940068 6.373461924301118E-5 54-55 0.0 0.0 0.0 0.4267457655781218 8.49794923240149E-5 56-57 0.0 0.0 0.0 0.49285981060620543 8.49794923240149E-5 58-59 0.0 0.0 0.0 0.5693201088247379 8.49794923240149E-5 60-61 0.0 0.0 0.0 0.6591221873381405 8.49794923240149E-5 62-63 0.0 0.0 0.0 0.7506026108249426 8.49794923240149E-5 64-65 0.0 0.0 0.0 0.8438251139043871 8.49794923240149E-5 66-67 0.0 0.0 0.0 0.9494971126092995 8.49794923240149E-5 68-69 0.0 0.0 0.0 1.0750755573911126 8.49794923240149E-5 70-71 0.0 0.0 0.0 1.2160352902835725 8.49794923240149E-5 72-73 0.0 0.0 0.0 1.3664914814432407 1.2746923848602236E-4 74-75 0.0 0.0 0.0 1.5138246762600014 1.2746923848602236E-4 76-77 0.0 0.0 0.0 1.67020818700927 1.2746923848602236E-4 78-79 0.0 0.0 0.0 1.8614332896113845 1.2746923848602236E-4 80-81 0.0 0.0 0.0 2.0779185463068126 1.2746923848602236E-4 82-83 0.0 0.0 0.0 2.3116758848170966 1.2746923848602236E-4 84-85 0.0 0.0 0.0 2.542628900080688 1.2746923848602236E-4 86-87 0.0 0.0 0.0 2.780274050364796 1.2746923848602236E-4 88-89 0.0 0.0 0.0 3.0504238564628388 1.2746923848602236E-4 90-91 0.0 0.0 0.0 3.355882641621511 1.2746923848602236E-4 92-93 0.0 0.0 0.0 3.677784958544879 1.2746923848602236E-4 94-95 0.0 0.0 0.0 4.011584404393609 1.2746923848602236E-4 96-97 0.0 0.0 0.0 4.344300361715209 1.2746923848602236E-4 98-99 0.0 0.0 0.0 4.704762123280599 1.2746923848602236E-4 100-101 0.0 0.0 0.0 5.125325630792149 1.2746923848602236E-4 102-103 0.0 0.0 0.0 5.570405721839178 1.2746923848602236E-4 104-105 0.0 0.0 0.0 6.026044514807465 1.2746923848602236E-4 106-107 0.0 0.0 0.0 6.474651254785939 1.2746923848602236E-4 108-109 0.0 0.0 0.0 6.951832349058364 1.2746923848602236E-4 110-111 0.0 0.0 0.0 7.472119290812145 1.2746923848602236E-4 112-113 0.0 0.0 0.0 8.038996239232567 1.2746923848602236E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2515 0.0 63.20111 1 GTATCAA 4660 0.0 57.615868 1 TCAACGC 5635 0.0 46.273426 4 ATCAACG 5685 0.0 45.971165 3 CAACGCA 5865 0.0 44.458782 5 AACGCAG 6060 0.0 42.816223 6 TATCAAC 6465 0.0 41.529766 2 GTACATG 12555 0.0 39.071667 1 TACATGG 12915 0.0 36.922367 2 ACATGGG 12845 0.0 35.68684 3 ACGCAGA 7650 0.0 33.83003 7 CGCAGAG 7750 0.0 33.090664 8 CATGGGG 6670 0.0 31.506512 4 GCAGAGT 8805 0.0 27.91 9 CATGGGA 6830 0.0 26.933279 4 TAAGGTG 1370 0.0 24.768843 5 ATGGGGA 3935 0.0 23.298471 5 GTAAGGT 1305 0.0 23.265432 4 GAGTACA 9355 0.0 23.16376 1 CAGAGTA 8435 0.0 22.25585 10-11 >>END_MODULE