FastQCFastQC Report
Fri 27 May 2016
SRR940152_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940152_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2353509
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60790.2582951669188433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47370.20127392756942933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27740.11786655585340867No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG25340.10766901677452688No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24840.1055445294664265No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42200.077.376331
GTATCAA83400.059.5139771
ATCAACG109600.044.998033
TCAACGC110200.044.6970834
CAACGCA112950.043.620755
TATCAAC114650.043.1746062
AACGCAG114800.042.9687166
ACGCAGA138450.035.579037
GTACATG122450.035.5712051
CGCAGAG139700.035.1755078
TACATGG126950.033.5955472
ACATGGG125950.032.5359883
TGGTATC18800.031.684262
TAAGGTG13400.031.5284445
GCAGAGT154250.031.0861249
CATGGGG64400.030.9823364
GTAAGGT12300.029.0536984
GTGGTAT21700.028.8316571
GAGTACT89350.027.132512-13
AGAGTAC144750.026.57092510-11
Produced by FastQC (version 0.11.5)