FastQCFastQC Report
Fri 27 May 2016
SRR940151_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940151_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1532035
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16390.106981890100422No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22850.075.018421
GTATCAA48300.060.9985771
TCAACGC60800.046.5992744
ATCAACG61700.045.9195483
CAACGCA64000.044.3623165
AACGCAG65650.043.2332276
TATCAAC68750.042.5945472
GTACATG126450.039.821091
TACATGG130700.037.3877372
CGCAGAG76750.036.901878
ACGCAGA77200.036.6831787
ACATGGG134950.034.491313
GCAGAGT82450.032.186839
CATGGGG47850.031.968854
CATGGGA89350.030.9100154
GTAAGGT6400.030.690914
AGGTAAG6150.030.0018632
ATGGGAT36800.029.7608815
TAAGGGT6200.028.8008563
TAAGGTG7300.028.537835
Produced by FastQC (version 0.11.5)