##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940151_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1532035 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.628486947099773 33.0 31.0 34.0 28.0 34.0 2 31.594294516770177 34.0 31.0 34.0 28.0 34.0 3 31.7521525291524 34.0 31.0 34.0 28.0 34.0 4 34.95864911702409 37.0 35.0 37.0 32.0 37.0 5 34.99612933124896 37.0 35.0 37.0 32.0 37.0 6 34.97140078392465 37.0 36.0 37.0 32.0 37.0 7 34.8580287003887 37.0 35.0 37.0 32.0 37.0 8 34.75311334271084 37.0 35.0 37.0 32.0 37.0 9 36.31046679742956 39.0 38.0 39.0 32.0 39.0 10-11 36.339796414572774 39.0 38.0 39.0 32.0 39.0 12-13 36.28487077645093 39.0 38.0 39.0 32.0 39.0 14-15 37.72011899205958 41.0 39.0 41.0 32.0 41.0 16-17 37.793635915628556 41.0 39.0 41.0 32.0 41.0 18-19 37.866820274993714 41.0 39.0 41.0 32.0 41.0 20-21 37.956905684269614 41.0 39.0 41.0 32.0 41.0 22-23 37.997397905400334 41.0 39.0 41.0 32.0 41.0 24-25 38.06068627674955 41.0 39.0 41.0 32.0 41.0 26-27 38.07464875149719 41.0 38.0 41.0 32.0 41.0 28-29 38.021431625256604 41.0 38.0 41.0 32.0 41.0 30-31 37.94866794818657 41.0 38.0 41.0 32.0 41.0 32-33 37.87450874164102 40.5 38.0 41.0 32.0 41.0 34-35 37.78834817742414 40.0 38.0 41.0 31.5 41.0 36-37 37.654598295730835 40.0 38.0 41.0 31.0 41.0 38-39 37.51148994637851 40.0 38.0 41.0 31.0 41.0 40-41 37.40209394693986 40.0 38.0 41.0 30.0 41.0 42-43 37.36497893324891 40.0 38.0 41.0 30.5 41.0 44-45 37.42961485866837 40.0 38.0 41.0 31.0 41.0 46-47 37.346219244338414 40.0 37.5 41.0 30.5 41.0 48-49 37.231119719849744 40.0 37.0 41.0 30.0 41.0 50-51 37.10865450201855 40.0 37.0 41.0 30.0 41.0 52-53 36.945919642828 40.0 36.5 41.0 30.0 41.0 54-55 36.77592483200449 40.0 36.0 41.0 30.0 41.0 56-57 36.5857506519107 40.0 35.5 41.0 30.0 41.0 58-59 36.35052919809273 39.5 35.0 41.0 30.0 41.0 60-61 36.11723916229068 39.0 35.0 41.0 30.0 41.0 62-63 35.839421096776505 39.0 35.0 41.0 30.0 41.0 64-65 35.535872874966955 38.0 35.0 41.0 29.0 41.0 66-67 35.20542513715418 37.5 35.0 40.0 29.0 41.0 68-69 34.86336408763508 37.0 34.5 40.0 29.0 41.0 70-71 34.73970699102827 36.5 35.0 39.5 29.0 41.0 72-73 34.50087041092404 36.0 35.0 39.0 29.0 41.0 74-75 34.157370099247075 36.0 35.0 39.0 29.0 41.0 76-77 33.78773624623459 35.0 34.0 37.5 29.0 39.5 78-79 33.43799064642779 35.0 34.0 37.0 28.5 39.0 80-81 33.11336686172314 35.0 34.0 36.5 28.0 39.0 82-83 32.82073842960507 35.0 34.0 36.0 28.0 37.5 84-85 32.55715078310874 35.0 34.0 36.0 27.0 37.0 86-87 32.34014105421873 35.0 34.0 35.5 27.0 37.0 88-89 32.15052299719001 35.0 34.0 35.0 27.0 36.0 90-91 32.00061976390879 35.0 34.0 35.0 26.5 36.0 92-93 31.852583981436457 35.0 34.0 35.0 26.0 36.0 94-95 31.747059629838745 35.0 34.0 35.0 25.5 36.0 96-97 31.61990228682765 35.0 34.0 35.0 25.0 35.5 98-99 31.529282947191156 35.0 33.5 35.0 25.0 35.0 100-101 31.4611758217012 35.0 33.0 35.0 25.0 35.0 102-103 30.696389769163236 34.5 31.5 35.0 23.5 35.0 104-105 31.25623696586566 35.0 33.0 35.0 24.0 35.0 106-107 31.304754460570418 35.0 33.0 35.0 24.0 35.0 108-109 31.258944149448283 35.0 33.0 35.0 24.0 35.0 110-111 31.165911026836852 35.0 33.0 35.0 23.0 35.0 112-113 31.0643516629842 35.0 33.0 35.0 21.5 35.0 114-115 30.932632087387034 35.0 33.0 35.0 19.5 35.0 116-117 30.844807070334554 35.0 33.0 35.0 19.0 35.0 118-119 30.706089612835214 35.0 33.0 35.0 15.0 35.0 120-121 30.54732561592914 35.0 33.0 35.0 2.0 35.0 122-123 30.31135352651865 35.0 33.0 35.0 2.0 35.0 124-125 28.319950588596214 33.0 29.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25917700274156985 1101 2 0.25961075571844106 1101 3 0.2936381033848967 1101 4 0.25259730634056865 1101 5 0.30793330030580535 1101 6 0.2829490369043839 1101 7 0.22837762239566928 1101 8 0.27331520519400954 1101 9 0.3083567346287097 1101 10-11 0.2920602874885887 1101 12-13 0.3858894677081821 1101 14-15 0.41833465029164074 1101 16-17 0.3356152387567093 1101 18-19 0.37560946373341864 1101 20-21 0.3261513655588075 1101 22-23 0.3325190860555196 1101 24-25 0.3738649770458409 1101 26-27 0.36786889990185756 1101 28-29 0.28597217462353086 1101 30-31 0.31936124052173653 1101 32-33 0.3332150117570478 1101 34-35 0.3445377565920609 1101 36-37 0.3953607625921691 1101 38-39 0.4485039237814874 1101 40-41 0.45164627887076847 1101 42-43 0.4114683596150712 1101 44-45 0.3133545102394777 1101 46-47 0.3299084911993262 1101 48-49 0.40161369331821106 1101 50-51 0.3475107099551167 1101 52-53 0.3701507310951868 1101 54-55 0.31079490889329264 1101 56-57 0.31730735441345814 1101 58-59 0.33139673096064115 1101 60-61 0.42109658422320706 1101 62-63 0.3129512778691321 1101 64-65 0.33396430827282586 1101 66-67 0.3301802581240878 1101 68-69 0.24840267611737943 1101 70-71 0.3330148722915851 1101 72-73 0.35807646572556706 1101 74-75 0.3273917487390179 1101 76-77 0.2515271495187861 1101 78-79 0.23816569203675897 1101 80-81 0.30780278164228747 1101 82-83 0.24910755377447913 1101 84-85 0.24025463601068964 1101 86-87 0.2744438421640254 1101 88-89 0.27822866418531333 1101 90-91 0.21722059590139509 1101 92-93 0.25770592151554084 1101 94-95 0.15443710441380532 1101 96-97 0.08212206348153117 1101 98-99 0.21652969372062003 1101 100-101 0.18510443371281582 1101 102-103 0.08437950564145069 1101 104-105 0.07496435112133426 1101 106-107 0.013948037518105139 1101 108-109 0.01069835829390442 1101 110-111 0.0723579666114027 1101 112-113 0.039871349250535815 1101 114-115 0.06235352088589963 1101 116-117 0.005697875416032616 1101 118-119 -0.04088319950909991 1101 120-121 -0.07284303873315778 1101 122-123 -0.06156272996090806 1101 124-125 -0.22803163028758178 1102 1 0.12322327874487726 1102 2 0.1314848602425478 1102 3 0.11563239337055009 1102 4 0.08374220978471669 1102 5 0.06818192603785178 1102 6 0.0725442971368011 1102 7 0.019101784712205472 1102 8 0.017826677362947407 1102 9 0.020745870879501638 1102 10-11 0.027435495720965264 1102 12-13 -0.0030937580292942357 1102 14-15 0.10758994734646166 1102 16-17 0.06116420107814946 1102 18-19 0.006376438012942742 1102 20-21 0.15224140344154335 1102 22-23 0.1236310140791943 1102 24-25 0.04416105040323259 1102 26-27 -0.010614528700621406 1102 28-29 0.23010050360307588 1102 30-31 0.2502370739989317 1102 32-33 0.08279569900932415 1102 34-35 0.27578185795270116 1102 36-37 0.22889984543206054 1102 38-39 0.09171872495187472 1102 40-41 0.19877659356126287 1102 42-43 0.14723505785799063 1102 44-45 0.0473316806977806 1102 46-47 0.22048681417617644 1102 48-49 0.14633723170437918 1102 50-51 -0.0013766653573981102 1102 52-53 0.0659502864571806 1102 54-55 0.17684584597184738 1102 56-57 0.08951285621733263 1102 58-59 0.02193603638243502 1102 60-61 0.12303751589494283 1102 62-63 0.14385662097387808 1102 64-65 0.004173517730279741 1102 66-67 0.07663592620716031 1102 68-69 0.0042994533242932675 1102 70-71 0.01911875143836994 1102 72-73 0.0064633384761947354 1102 74-75 0.12783557616619845 1102 76-77 0.1080286440598357 1102 78-79 0.05515550409526071 1102 80-81 0.0641130660651541 1102 82-83 -0.04931802982386557 1102 84-85 -0.18289556821258657 1102 86-87 -0.08240116459668201 1102 88-89 0.0446273197556053 1102 90-91 -0.010222503314928133 1102 92-93 -0.07618061692408773 1102 94-95 0.025132795526218388 1102 96-97 5.020922769709557E-4 1102 98-99 0.026744121584364677 1102 100-101 -0.03133794956094249 1102 102-103 -0.02713884465863714 1102 104-105 0.04693086218514253 1102 106-107 0.0034514169948280937 1102 108-109 0.013132847797063363 1102 110-111 0.0017340012009796624 1102 112-113 0.07371957118046168 1102 114-115 -0.003933274136329601 1102 116-117 -0.0558012180446994 1102 118-119 -0.0707912179781438 1102 120-121 0.04303920930895444 1102 122-123 -0.015097334873658497 1102 124-125 -0.09897356357785725 1103 1 0.17472080083063446 1103 2 0.2125189624387751 1103 3 0.31602155466358184 1103 4 0.2501592456422159 1103 5 0.14765474080478214 1103 6 0.18151646636722063 1103 7 -0.06018052233194027 1103 8 0.0730196113220174 1103 9 0.2534333173402672 1103 10-11 0.30319630035550915 1103 12-13 0.27469080795469836 1103 14-15 0.3524309106235819 1103 16-17 0.16607636606921972 1103 18-19 0.2508405463579706 1103 20-21 0.2160660890381152 1103 22-23 0.13977862384229667 1103 24-25 0.1971832036047232 1103 26-27 0.11859131248513677 1103 28-29 0.04329235320096103 1103 30-31 0.1299158998757477 1103 32-33 0.22642976301641227 1103 34-35 0.29964656330951556 1103 36-37 0.17427427412787466 1103 38-39 0.0815657581992042 1103 40-41 0.15058383302990563 1103 42-43 0.1589963915346786 1103 44-45 0.044792453624836526 1103 46-47 0.006757075876855367 1103 48-49 0.11513425645549802 1103 50-51 0.044773283052236934 1103 52-53 0.0518373867632107 1103 54-55 0.005891879644856601 1103 56-57 0.010618790773769149 1103 58-59 0.07430420065877996 1103 60-61 -0.08616755450094615 1103 62-63 0.004517301756507663 1103 64-65 0.07141705054052494 1103 66-67 -0.050561700811130095 1103 68-69 -0.06117673715189653 1103 70-71 0.056130679411566575 1103 72-73 -0.12038918489712813 1103 74-75 0.013663859950000301 1103 76-77 -0.17660653584076869 1103 78-79 0.027987132056011887 1103 80-81 -0.002859814394099658 1103 82-83 9.357177039106546E-4 1103 84-85 0.029789662227301505 1103 86-87 -0.08369530326528007 1103 88-89 0.06887282158360364 1103 90-91 0.11253695068555913 1103 92-93 0.16964419830532407 1103 94-95 0.04242739316514488 1103 96-97 -0.06503979051742803 1103 98-99 -0.03336018905796223 1103 100-101 -0.11726328279617348 1103 102-103 -0.022438778265602366 1103 104-105 0.13058564992888222 1103 106-107 0.1449719401509313 1103 108-109 0.1242376086507484 1103 110-111 0.10561195012914482 1103 112-113 0.0848623021940682 1103 114-115 0.12863754656218163 1103 116-117 0.09632532988528908 1103 118-119 0.11378864899053198 1103 120-121 0.05974016736572452 1103 122-123 -0.02528117701589494 1103 124-125 0.2982858404964688 1104 1 0.19370107053414287 1104 2 0.16540120823326632 1104 3 0.17888145272116773 1104 4 0.22744797534174666 1104 5 0.12331713691076374 1104 6 0.23022994082803905 1104 7 0.1923871714122285 1104 8 0.2260365558047326 1104 9 0.33134043647206823 1104 10-11 0.3377951125579912 1104 12-13 0.31744042146130624 1104 14-15 0.29601244159662343 1104 16-17 0.36754848497142945 1104 18-19 0.4177195930586137 1104 20-21 0.45791528773397516 1104 22-23 0.3822668759737482 1104 24-25 0.2796574501339322 1104 26-27 0.20352801940834553 1104 28-29 0.37494769293248 1104 30-31 0.3373231797275764 1104 32-33 0.3799499142463034 1104 34-35 0.31191868527304933 1104 36-37 0.4510532917458434 1104 38-39 0.29413903887758863 1104 40-41 0.44155213291816864 1104 42-43 0.3851830410578714 1104 44-45 0.452336345498523 1104 46-47 0.3060158504249273 1104 48-49 0.4413939953237218 1104 50-51 0.46408800347592916 1104 52-53 0.48308556909626077 1104 54-55 0.42845161392107656 1104 56-57 0.4525625081514377 1104 58-59 0.3441148571618129 1104 60-61 0.37178502543812897 1104 62-63 0.25142726792876147 1104 64-65 0.2532755863045537 1104 66-67 0.2902393737170428 1104 68-69 0.37178579212211105 1104 70-71 0.36068938814095475 1104 72-73 0.30045428910449345 1104 74-75 0.2705690810548447 1104 76-77 0.03981063211930547 1104 78-79 0.25882318232311263 1104 80-81 0.0996240753366564 1104 82-83 0.11517130264827102 1104 84-85 0.045865491466102526 1104 86-87 0.1476803070881516 1104 88-89 0.08505388417257365 1104 90-91 0.13717494796097895 1104 92-93 0.21123433162640382 1104 94-95 0.22303269539532522 1104 96-97 0.344581981688723 1104 98-99 0.3046321116874715 1104 100-101 0.3390476568802434 1104 102-103 0.27642776288637805 1104 104-105 0.25312701620217126 1104 106-107 0.2450163294913672 1104 108-109 0.18695477201882582 1104 110-111 0.3055782754570906 1104 112-113 0.3424142300690427 1104 114-115 0.3077638418890558 1104 116-117 0.30684560356853297 1104 118-119 0.36275544882522226 1104 120-121 0.32817131031470836 1104 122-123 0.296738614507273 1104 124-125 0.40332594396954846 1105 1 -0.1027580625682738 1105 2 -0.07142234863943386 1105 3 -0.05345777024716014 1105 4 -0.13769209626754986 1105 5 -0.0828830273154324 1105 6 -0.005296687574755765 1105 7 -0.1386832582852051 1105 8 -0.008359454415639789 1105 9 0.06056623915888082 1105 10-11 0.05501083242310756 1105 12-13 -0.032532172770771695 1105 14-15 -0.010633613431657807 1105 16-17 2.8358061026523274E-4 1105 18-19 0.08946716405309729 1105 20-21 0.03744786829443569 1105 22-23 0.11768929993576904 1105 24-25 0.026311758153923392 1105 26-27 0.09079152743000662 1105 28-29 0.09714480188447538 1105 30-31 0.025153247293737024 1105 32-33 0.05115728941980535 1105 34-35 0.11666353027937504 1105 36-37 0.003388513263651305 1105 38-39 0.12782281174526133 1105 40-41 0.05323282550256181 1105 42-43 0.024293051770087004 1105 44-45 0.020019630689404266 1105 46-47 -0.13410734006495773 1105 48-49 -0.14534921992513716 1105 50-51 -0.15175565651748713 1105 52-53 -0.1179023932067409 1105 54-55 -0.14000632708253846 1105 56-57 -0.17595751785799507 1105 58-59 -0.06972766075093517 1105 60-61 -0.11855126313314202 1105 62-63 -0.04553173387605369 1105 64-65 -0.07384070168316725 1105 66-67 -0.239004247929131 1105 68-69 -0.21944196544001215 1105 70-71 -0.1380521348277881 1105 72-73 -0.24738378136272132 1105 74-75 -0.36012590004247613 1105 76-77 -0.27215412177064024 1105 78-79 -0.2473761482675627 1105 80-81 -0.22346877498825535 1105 82-83 -0.34869528649029746 1105 84-85 -0.21713797228950682 1105 86-87 -0.21635623949331517 1105 88-89 -0.33818756168488306 1105 90-91 -0.20210397814974002 1105 92-93 -0.2519697256776965 1105 94-95 -0.2662453970798637 1105 96-97 -0.27907263477640853 1105 98-99 -0.18261065965047507 1105 100-101 -0.2094276237423145 1105 102-103 -0.20940064157690585 1105 104-105 -0.2869595985290019 1105 106-107 -0.27816144745277427 1105 108-109 -0.27976055812168 1105 110-111 -0.21545830863797732 1105 112-113 -0.18172712533895918 1105 114-115 -0.20760672994323315 1105 116-117 -0.12773172237879038 1105 118-119 -0.14705602450052524 1105 120-121 -0.10616019735682869 1105 122-123 -0.05585847277020051 1105 124-125 -0.02237076692148321 1106 1 -0.03405387764021839 1106 2 0.040193832565236676 1106 3 -0.029519516358938347 1106 4 0.16452641980893645 1106 5 0.08849346106838141 1106 6 0.07548910151107435 1106 7 0.05743821710555608 1106 8 0.13845821614248166 1106 9 0.11199547547738575 1106 10-11 0.08027942025229606 1106 12-13 0.09019792070166943 1106 14-15 0.15328253286628524 1106 16-17 0.19115547294242674 1106 18-19 0.20600325372458883 1106 20-21 0.09708406268608627 1106 22-23 0.21817605477423285 1106 24-25 0.19960617695701188 1106 26-27 0.20185114035324858 1106 28-29 0.1740908516071613 1106 30-31 0.3191454126740325 1106 32-33 0.33583129942640255 1106 34-35 0.384301120939881 1106 36-37 0.3390302151602427 1106 38-39 0.2720379922905778 1106 40-41 0.32075793894940574 1106 42-43 0.2982700909044027 1106 44-45 0.2516897233861357 1106 46-47 0.2756161211303976 1106 48-49 0.2307687647934742 1106 50-51 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2216 40-41 -0.6989139566378668 2216 42-43 -0.6190558824916508 2216 44-45 -0.6567332194396442 2216 46-47 -0.6768766380073572 2216 48-49 -0.7065761537138684 2216 50-51 -0.6698081480914837 2216 52-53 -0.5819896202465245 2216 54-55 -0.6629074986154961 2216 56-57 -0.7876118622432884 2216 58-59 -0.6898733449994268 2216 60-61 -0.7295976538133999 2216 62-63 -0.7164347499940291 2216 64-65 -0.8345189268000581 2216 66-67 -0.6295693379713043 2216 68-69 -0.631156820439962 2216 70-71 -0.5145923672676389 2216 72-73 -0.5291736457952183 2216 74-75 -0.5282472471590438 2216 76-77 -0.4789914257340442 2216 78-79 -0.6480291092140931 2216 80-81 -0.5701229334912625 2216 82-83 -0.5648305012159653 2216 84-85 -0.46718135800472993 2216 86-87 -0.5963840981057196 2216 88-89 -0.4597739522544053 2216 90-91 -0.4919881062550644 2216 92-93 -0.5639030516812049 2216 94-95 -0.5061058350817937 2216 96-97 -0.5400555486353973 2216 98-99 -0.5602158789085117 2216 100-101 -0.6125381066328401 2216 102-103 -0.6196241669965055 2216 104-105 -0.5875319577548161 2216 106-107 -0.7455728824560666 2216 108-109 -0.7261810586433377 2216 110-111 -0.728718913222071 2216 112-113 -0.6373816247579782 2216 114-115 -0.6930049253822332 2216 116-117 -0.6666997672373256 2216 118-119 -0.8780975743316084 2216 120-121 -0.9778181328295723 2216 122-123 -0.9574338053215108 2216 124-125 -1.0876027124128882 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19656.0 3 3947.0 4 2843.0 5 2295.0 6 3069.0 7 3205.0 8 3134.0 9 3338.0 10 4121.0 11 4691.0 12 5297.0 13 5413.0 14 5389.0 15 5239.0 16 4836.0 17 4801.0 18 4304.0 19 3992.0 20 3974.0 21 3866.0 22 4357.0 23 5482.0 24 7963.0 25 11040.0 26 16262.0 27 15052.0 28 14827.0 29 20345.0 30 22335.0 31 24606.0 32 32189.0 33 44139.0 34 63673.0 35 101835.0 36 220829.0 37 523068.0 38 305130.0 39 1493.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.737599783767834 19.472581539178694 13.502889297878745 22.286929379174726 2 14.883434605612583 21.74764532158627 39.99643566838399 23.37248440441716 3 17.72345506223097 28.589818744883715 29.829476836867784 23.857249356017533 4 11.949073632715319 17.50166180639988 38.47555218333284 32.07371237755196 5 12.491732140280359 41.09973471025437 33.35905919101972 13.049473958445557 6 29.76926843600039 37.77618907713903 18.713217771684132 13.741324715176445 7 27.944107346273988 34.566949437450425 21.578729196110626 15.910214020164961 8 26.914187207616607 35.09449283005912 20.641557785435054 17.349762176889218 9 28.378316625626375 16.525876273315284 19.524058836892916 35.57174826416543 10-11 24.11761768605933 28.445009126770564 27.46967017323077 19.967703013939335 12-13 25.348065137088284 26.03087921491884 27.549774179503633 21.071281468489246 14-15 22.53183076082422 26.679801772194704 25.638133065408518 25.150234401572558 16-17 21.899106402699722 30.277543880261877 25.967846162884058 21.855503554154346 18-19 22.227847043590625 28.901779195189242 27.59415510341566 21.27621865780447 20-21 23.775977284409958 27.847061415318336 26.91533057547793 21.461630724793775 22-23 23.34123025538313 28.28652674569681 26.84566211595519 21.526580882964875 24-25 22.637427700337913 28.26750736256251 27.105784266718803 21.98928067038078 26-27 22.409736620778766 28.610233281909203 26.733573515670873 22.246456581641162 28-29 22.311118857336076 28.571461210016913 26.996029189342323 22.121390743304683 30-31 22.38863644448622 28.124786841951526 27.098495091939046 22.38808162162321 32-33 22.271489443919354 28.4519401987457 26.76441637380484 22.512153983530112 34-35 22.792213217478484 28.391704611914108 26.572747827721617 22.243334342885788 36-37 23.028251346180333 27.858899281754283 26.448910424582895 22.663938947482485 38-39 22.517721700761086 27.948884479314888 26.781961071004297 22.75143274891973 40-41 22.453242879166066 27.951943612376212 27.029155736080906 22.565657772376817 42-43 22.61291344915137 27.784481444623705 26.86399926366648 22.738605842558446 44-45 22.13956433265286 28.105651534659366 27.01568356758327 22.73910056510451 46-47 22.738388143571772 28.230629280332874 26.540495514932488 22.49048706116286 48-49 22.416007937388624 28.114315367593782 26.483136312900214 22.986540382117376 50-51 22.5490701670096 28.061646804466122 26.548154956498127 22.841128072026155 52-53 22.68504798446951 27.842578824168353 26.819604243991662 22.652768947370483 54-55 22.05708414709292 28.146508057525406 26.903446772290053 22.892961023091623 56-57 22.40063477805616 28.19921873470011 26.650331554684005 22.74981493255973 58-59 22.720533559305657 27.527189471395808 26.59554327041406 23.15673369888448 60-61 22.342420553457725 27.915052496564957 26.941197972591475 22.801328977385843 62-63 22.31881427963885 27.97263189983612 27.074385603253937 22.634168217271096 64-65 22.15171902404891 28.13512767272649 26.687872021138343 23.02528128208626 66-67 22.20371713919902 28.25683749762975 26.61471535951264 22.92473000365859 68-69 22.350341784524804 27.96817299894095 26.975733217147805 22.705751999386436 70-71 22.177642293670505 27.961461008972194 26.928140839646076 22.932755857711225 72-73 22.180327820700228 28.19657492869738 26.607601108727557 23.01549614187483 74-75 22.104146920359387 28.487817997143477 26.621808278696445 22.78622680380069 76-77 22.422436276157867 27.98584023423347 26.917851815342033 22.673871674266632 78-79 22.505282286569187 27.89184383622268 26.825007979728554 22.77786589747958 80-81 22.337581549064232 28.263031449894953 26.73343879622174 22.66594820481907 82-83 22.39841385106192 27.9453659810217 26.948017721787522 22.708202446128865 84-85 22.2904901945746 28.00318791776556 26.933499212974642 22.772822674685198 86-87 22.586555605345016 27.93796893159396 26.681381573772693 22.794093889288327 88-89 22.495065132418944 27.963857969811144 26.710329800594796 22.830747097175113 90-91 22.356563998289765 27.92608137967499 26.96711065272805 22.750243969307192 92-93 22.40529070340556 27.76121069134147 26.867110278694806 22.966388326558164 94-95 22.095089211408357 27.588860567806872 27.09618252846704 23.219867692317735 96-97 22.45972497909635 27.61171622730379 26.887578498840426 23.040980294759432 98-99 22.20234250646952 28.002374686804725 26.703542415215747 23.091740391510005 100-101 21.88471542751961 27.782720368659987 27.257079635909104 23.07548456791131 102-103 22.005458757918806 27.654584576642744 27.034167333808302 23.30578933163015 104-105 21.718205947362307 27.869334019189168 27.1688993972215 23.243560636227027 106-107 21.959028351179967 27.860884379273323 26.905455815304478 23.274631454242233 108-109 22.080859771480416 27.956867826126686 26.57383806505726 23.388434337335635 110-111 21.968705512370214 28.097872948714354 26.79211919894362 23.141302339971816 112-113 21.645408783559947 28.334045392725503 27.061248748728328 22.959297074986218 114-115 21.874821515584404 28.43678167672054 26.572123369733546 23.11627343796151 116-117 21.64017675894075 28.43172131094039 26.834657284779016 23.093444645339844 118-119 21.479708852161952 28.621812979846094 26.556844882278085 23.34163328571387 120-121 21.47145177166639 28.82378244443226 26.797384341033055 22.907381442868296 122-123 21.494946752178233 28.60976694715426 26.81907620226568 23.07621009840183 124-125 21.71794056945697 28.747363851955686 26.402861884295923 23.13183369429142 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1227.0 1 1099.0 2 1259.5 3 1343.0 4 978.5 5 740.5 6 602.5 7 559.5 8 616.0 9 619.0 10 528.0 11 462.0 12 463.0 13 460.0 14 469.0 15 535.5 16 607.5 17 689.5 18 842.5 19 1030.0 20 1264.0 21 1711.0 22 2395.0 23 3270.0 24 4197.0 25 5622.0 26 7396.0 27 9292.5 28 11731.5 29 14764.0 30 18812.5 31 22815.5 32 27465.5 33 31317.5 34 34488.0 35 36756.0 36 39856.0 37 43896.0 38 44908.5 39 45606.5 40 47066.5 41 49550.5 42 51065.5 43 52513.0 44 55460.0 45 58928.5 46 58485.0 47 56386.0 48 55936.5 49 53935.0 50 52789.0 51 50851.5 52 49375.0 53 49816.0 54 49680.5 55 47888.5 56 43093.5 57 38185.5 58 35878.0 59 32037.0 60 25949.0 61 21455.0 62 18112.5 63 13751.5 64 10083.0 65 8159.0 66 5891.5 67 4416.0 68 3505.0 69 2378.5 70 1677.5 71 1307.5 72 1094.0 73 942.5 74 756.0 75 520.5 76 332.0 77 202.0 78 143.0 79 78.5 80 48.5 81 39.5 82 24.5 83 17.0 84 17.0 85 12.5 86 9.0 87 9.5 88 7.5 89 9.5 90 10.0 91 8.0 92 8.5 93 5.0 94 3.0 95 6.0 96 6.5 97 6.0 98 6.5 99 5.0 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02271488575652645 2 0.012467078102001587 3 0.010508898295404478 4 0.03714014366512514 5 0.032766875430391605 6 0.025129974184662885 7 0.011096352237383611 8 9.138172430786503E-4 9 0.013511440665520043 10-11 2.2845431076966259E-4 12-13 0.004307995574513637 14-15 0.01710143697761474 16-17 0.0016318165054975897 18-19 0.008779172799577033 20-21 0.00310045136044542 22-23 2.2845431076966259E-4 24-25 0.008452809498477515 26-27 2.9372697098956616E-4 28-29 0.00691890198330978 30-31 9.464535731886021E-4 32-33 0.001762361825937397 34-35 1.9581798065971078E-4 36-37 0.007049447303749588 38-39 9.790899032985538E-4 40-41 0.013674622316069801 42-43 0.007865355556498383 44-45 0.0011422715538483127 46-47 0.011357442878263225 48-49 0.0029372697098956615 50-51 0.009595081052325828 52-53 0.00528708547781219 54-55 0.003328905671215083 56-57 0.010508898295404478 58-59 0.01853743550245262 60-61 0.0019581798065971077 62-63 0.006886265653199828 64-65 0.002088725127036915 66-67 0.0015339075151677346 68-69 1.6318165054975898E-4 70-71 0.005221812817592287 72-73 5.548176118691805E-4 74-75 0.00672308400265007 76-77 0.015012711850577825 78-79 0.0018276344861573007 80-81 0.0038184506228643605 82-83 0.0014686348549478308 84-85 3.2636330109951795E-5 86-87 0.0018602708162672524 88-89 0.005156540157372384 90-91 0.005221812817592287 92-93 0.014098894607499177 94-95 0.0 96-97 3.9163596131942157E-4 98-99 0.0028393607195658062 100-101 0.0 102-103 9.790899032985539E-5 104-105 0.008420173168367562 106-107 0.0 108-109 0.0 110-111 5.221812817592287E-4 112-113 0.006102993730560986 114-115 0.0025129974184662884 116-117 3.2636330109951796E-4 118-119 0.006429357031660503 120-121 0.002023452466817011 122-123 3.2636330109951795E-5 124-125 0.0754225588840986 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1532035.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.47471745501025 #Duplication Level Percentage of deduplicated Percentage of total 1 64.41550319999324 23.49537278942062 2 14.284108678284474 10.420176562741721 3 6.727506767709669 7.361517255866383 4 3.846681580217046 5.612264951112364 5 2.4536071976944185 4.4747314640741696 6 1.7147825128133958 3.752772459099666 7 1.196973027536402 3.0561477086460926 8 0.8509624966304575 2.483089330352484 9 0.6740062553401477 2.212576895379724 >10 3.509703001908098 22.63437208287067 >50 0.2113186967990461 5.310425052830218 >100 0.10857574795149336 7.278447360975764 >500 0.004658336147823529 1.1869624923440818 >1k 0.0016125009742466066 0.7211435942860179 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1639 0.106981890100422 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 6.527266021990359E-5 6 0.0 0.0 0.0 0.0 6.527266021990359E-5 7 0.0 0.0 0.0 0.0 6.527266021990359E-5 8 0.0 0.0 0.0 0.0 6.527266021990359E-5 9 0.0 0.0 0.0 0.0 6.527266021990359E-5 10-11 0.0 0.0 0.0 0.0 6.527266021990359E-5 12-13 6.527266021990359E-5 0.0 0.0 6.527266021990359E-5 6.527266021990359E-5 14-15 6.527266021990359E-5 0.0 0.0 6.527266021990359E-5 6.527266021990359E-5 16-17 6.527266021990359E-5 0.0 0.0 1.3054532043980718E-4 6.527266021990359E-5 18-19 6.527266021990359E-5 0.0 0.0 3.5899963120946977E-4 6.527266021990359E-5 20-21 6.527266021990359E-5 0.0 0.0 4.5690862153932517E-4 6.527266021990359E-5 22-23 6.527266021990359E-5 0.0 0.0 8.485445828587466E-4 6.527266021990359E-5 24-25 6.527266021990359E-5 0.0 0.0 0.0016970891657174935 6.527266021990359E-5 26-27 6.527266021990359E-5 0.0 0.0 0.0026109064087961437 6.527266021990359E-5 28-29 6.527266021990359E-5 0.0 0.0 0.005058631167042528 6.527266021990359E-5 30-31 6.527266021990359E-5 0.0 0.0 0.014033621947279272 6.527266021990359E-5 32-33 6.527266021990359E-5 0.0 0.0 0.029372697098956617 6.527266021990359E-5 34-35 6.527266021990359E-5 0.0 0.0 0.049280858466027214 6.527266021990359E-5 36-37 6.527266021990359E-5 0.0 0.0 0.08132973463399987 6.527266021990359E-5 38-39 6.527266021990359E-5 0.0 0.0 0.1360282238982791 6.527266021990359E-5 40-41 6.527266021990359E-5 0.0 0.0 0.20544569804214657 6.527266021990359E-5 42-43 9.790899032985538E-5 0.0 0.0 0.2647785461820389 6.527266021990359E-5 44-45 1.3054532043980718E-4 0.0 0.0 0.33928728782305884 6.527266021990359E-5 46-47 1.3054532043980718E-4 0.0 0.0 0.4253492903230017 6.527266021990359E-5 48-49 1.3054532043980718E-4 0.0 0.0 0.5226055540506581 6.527266021990359E-5 50-51 1.3054532043980718E-4 0.0 0.0 0.6385950712614268 6.527266021990359E-5 52-53 1.3054532043980718E-4 0.0 0.0 0.7487100490524041 6.527266021990359E-5 54-55 1.3054532043980718E-4 0.0 0.0 0.8528199421031504 6.527266021990359E-5 56-57 1.3054532043980718E-4 0.0 0.0 0.9723341829657939 6.527266021990359E-5 58-59 1.3054532043980718E-4 0.0 0.0 1.1200462130434357 6.527266021990359E-5 60-61 1.3054532043980718E-4 0.0 0.0 1.2898204022754047 6.527266021990359E-5 62-63 1.3054532043980718E-4 0.0 0.0 1.456330958496379 6.527266021990359E-5 64-65 1.3054532043980718E-4 0.0 0.0 1.6163468850254725 6.527266021990359E-5 66-67 1.3054532043980718E-4 0.0 0.0 1.7962709729216368 6.527266021990359E-5 68-69 1.3054532043980718E-4 0.0 0.0 2.009549390190172 6.527266021990359E-5 70-71 1.3054532043980718E-4 0.0 0.0 2.252526867858763 6.527266021990359E-5 72-73 1.3054532043980718E-4 0.0 0.0 2.509896967105843 6.527266021990359E-5 74-75 1.3054532043980718E-4 0.0 0.0 2.760380800699723 6.527266021990359E-5 76-77 1.3054532043980718E-4 0.0 0.0 3.0184688992092217 6.527266021990359E-5 78-79 1.3054532043980718E-4 0.0 0.0 3.328122399292444 6.527266021990359E-5 80-81 1.3054532043980718E-4 0.0 0.0 3.6820307630047617 6.527266021990359E-5 82-83 1.3054532043980718E-4 0.0 0.0 4.064822278864386 6.527266021990359E-5 84-85 1.3054532043980718E-4 0.0 0.0 4.445981978218513 6.527266021990359E-5 86-87 1.3054532043980718E-4 0.0 0.0 4.831417036817044 6.527266021990359E-5 88-89 1.3054532043980718E-4 0.0 0.0 5.243777067756285 6.527266021990359E-5 90-91 1.3054532043980718E-4 0.0 0.0 5.709334316774747 6.527266021990359E-5 92-93 1.3054532043980718E-4 0.0 0.0 6.231841961835076 6.527266021990359E-5 94-95 1.3054532043980718E-4 0.0 0.0 6.745374616115167 6.527266021990359E-5 96-97 1.3054532043980718E-4 0.0 0.0 7.2505850062172215 6.527266021990359E-5 98-99 1.3054532043980718E-4 0.0 0.0 7.789280271012085 6.527266021990359E-5 100-101 1.3054532043980718E-4 0.0 0.0 8.400721915622032 6.527266021990359E-5 102-103 1.3054532043980718E-4 0.0 0.0 9.068852865632966 6.527266021990359E-5 104-105 1.9581798065971078E-4 0.0 0.0 9.73593945308038 6.527266021990359E-5 106-107 1.9581798065971078E-4 0.0 0.0 10.394736412679867 6.527266021990359E-5 108-109 1.9581798065971078E-4 0.0 0.0 11.06404227057476 6.527266021990359E-5 110-111 1.9581798065971078E-4 0.0 0.0 11.797968062087353 6.527266021990359E-5 112-113 1.9581798065971078E-4 0.0 0.0 12.596513787217656 6.527266021990359E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2285 0.0 75.01842 1 GTATCAA 4830 0.0 60.998577 1 TCAACGC 6080 0.0 46.599274 4 ATCAACG 6170 0.0 45.919548 3 CAACGCA 6400 0.0 44.362316 5 AACGCAG 6565 0.0 43.233227 6 TATCAAC 6875 0.0 42.594547 2 GTACATG 12645 0.0 39.82109 1 TACATGG 13070 0.0 37.387737 2 CGCAGAG 7675 0.0 36.90187 8 ACGCAGA 7720 0.0 36.683178 7 ACATGGG 13495 0.0 34.49131 3 GCAGAGT 8245 0.0 32.18683 9 CATGGGG 4785 0.0 31.96885 4 CATGGGA 8935 0.0 30.910015 4 GTAAGGT 640 0.0 30.69091 4 AGGTAAG 615 0.0 30.001863 2 ATGGGAT 3680 0.0 29.760881 5 TAAGGGT 620 0.0 28.800856 3 TAAGGTG 730 0.0 28.53783 5 >>END_MODULE