FastQCFastQC Report
Fri 27 May 2016
SRR940151_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940151_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1532035
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95000.6200902720890842No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74240.4845842294725643No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48110.3140267683179562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42950.089.618291
GTATCAA94500.065.195261
ATCAACG130950.046.9479183
TCAACGC131900.046.6067354
TATCAAC132250.046.531472
CAACGCA135500.045.4118775
AACGCAG138700.044.3627136
ACGCAGA158850.038.6503227
TGGTATC12850.038.4784132
CGCAGAG160700.038.205378
GTGGTAT13550.037.8169481
GTACATG117600.036.530471
GCAGAGT170550.035.4407279
TACATGG123750.034.515652
ACATGGG121900.033.5733873
CATGGGA79600.031.5800724
GAGTACT103750.030.04718812-13
ATGGGAT33350.029.621055
AGAGTAC166650.028.9518410-11
AGTACTT108550.028.82813312-13
Produced by FastQC (version 0.11.5)