FastQCFastQC Report
Fri 27 May 2016
SRR940150_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940150_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587263
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38300.058.5851331
GGTATCA27550.051.416231
ATCAACG47350.045.3751833
TCAACGC47800.044.9480134
CAACGCA50200.042.7991035
AACGCAG51050.041.9619646
TATCAAC53300.041.5381282
GTACATG98400.035.4443781
ACGCAGA62200.034.1474657
TACATGG99000.034.0862352
CGCAGAG62550.033.7704168
ACATGGG102200.031.4466273
TGGTATC10150.028.7316912
GCAGAGT70900.028.5343539
CATGGGA66350.027.806864
CATGGGG40150.025.9408174
GAGTACA75250.025.0724981
TAAGGTG7450.023.9660345
ATGGGGA20850.023.4066335
GTATAGG5100.023.3402041
Produced by FastQC (version 0.11.5)