FastQCFastQC Report
Fri 27 May 2016
SRR940150_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940150_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587263
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69010.4347735693454708No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55770.35135954155045507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32860.20702303272992564No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44750.068.965061
GTATCAA75250.062.7854541
ATCAACG98700.047.427843
TCAACGC99750.046.8688964
TATCAAC101750.045.951992
CAACGCA103300.045.336345
AACGCAG104900.044.6434366
ACGCAGA121750.038.259717
CGCAGAG122600.037.8488778
TGGTATC12550.037.0185972
GCAGAGT135100.033.77459
GTGGTAT14600.033.4621121
GTACATG95450.032.8947831
TACATGG98050.031.5274492
GAGTACT79500.029.93241512-13
ACATGGG100250.028.931613
CAGAGTA132850.027.8981510-11
AGAGTAC129450.027.52793710-11
GTACTTT86650.027.22221814-15
AGTACTT83400.026.99906512-13
Produced by FastQC (version 0.11.5)