FastQCFastQC Report
Fri 27 May 2016
SRR940149_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940149_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844708
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35920.4252357027517201No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12060.14277122982142942No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11150.13199827632744096No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT9060.10725599852256637No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11550.080.912281
GTATCAA22400.057.1330221
ATCAACG29950.041.138133
GTACATG68500.040.5810281
TCAACGC30650.040.1985974
CAACGCA31050.039.8724375
AACGCAG32000.038.681856
TACATGG72250.038.060562
GTATACG801.1942871E-437.2029041
TATCAAC34500.036.7478182
ACATGGG72550.035.934183
ATATAGG6150.035.8094522
AGGCTTA5650.034.7583666
CTTACTA5550.034.308329
CATGGGG26650.033.5015644
TATAGGC6000.032.736613
TAAGGTG4250.032.2114265
CATGGGA47250.031.9965364
ACGCAGA39700.031.1719447
CGCAGAG40650.030.4470528
Produced by FastQC (version 0.11.5)